Addgene

We narrowed to 1,954 results for: Endog

Showing: 621 - 640 of 1954 results

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pCMV-TadA8e-BLM

Plasmid

#221226

Purpose

Express HACE Editor as a fusion of TadA-8e adenosine deaminase and BLM helicase.

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

TadA8e-BLM

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-mCAMK-RFP

Plasmid

#22908

Depositor

Edward Callaway

Article

Nathanson et al Neuroscience. 2009 Jun 30. 161(2):441-50

Insert

mouse alpha-calcium/calmodulin-dependent protein kinase II promoter (Camk2a Mouse)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pUC57-Amp_CRISPIE donor B2

Plasmid

#172843

Purpose

CRISPIE donor B2 (Zhong et al, eLife 2021), mRuby3 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 or DRS-2 sgRNA (with SpCas9).

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

CRISPIE designer exon (phase 0-0) encoding mRuby3

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pX330_DRS-1 sgRNA / hSpCas9

Plasmid

#172841

Purpose

Mammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

DRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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dimericTomato-2xrGBD

Plasmid

#176098

Purpose

Visualization of active RhoA

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

rhotekin (Rtkn Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMVdel

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pSP2-96-merTetO-EFS-BLaR

Plasmid

#118713

Purpose

Contains an optimized 96-mer TetO repeat for imaging of desired loci

Depositor

Huimin Zhao

Article

Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

Inserts

Blasticidin resistance gene
EFS-NS promoter

Use

Tags

Expression

Mammalian

Mutation

Promoter

EFS-NS

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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U6-BbsI-GGsite-mCherry-Puro

Plasmid

#221232

Purpose

Cloning vector for sgRNA with mCherry and Puro

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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CaMKIIP-mEGFP-P2A-paAIP2 (R5AR6A)/pAAV

Plasmid

#91719

Purpose

AAV-mediated expression of mEGFP and paAIP2 (R5AR6A), the functional mutant of paAIP2

Depositor

Ryohei Yasuda

Article

Murakoshi et al Neuron. 2017 Apr 5;94(1):37-47.e5. doi:

Insert

mEGFP-P2A-paAIP2(R5AR6A)

Use

AAV

Tags

Expression

Mutation

Promoter

CaMKII 1.3

Available since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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pT2/LTR7-GFP

Plasmid

#62541

Purpose

LTR7-driving reporter

Depositor

Zsuzsanna Izsvak

Article

Wang et al Nature. 2014 Dec 18;516(7531):405-9. doi

Insert

LTR7-GFP

Use

Sleeping beauty transposon

Tags

Expression

Mammalian

Mutation

Promoter

LTR7

Available since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mEGFP-mTrim21

Plasmid

#105519

Purpose

T7 promotor drives in vitro transcription of mEGFP-tagged wildtype mouse TRIM21 mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mEGFP

Expression

Mutation

Promoter

T7

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn.flex.H2B.GFP

Plasmid

#170368

Purpose

Cre-inducible AAV vector expressing nuclear GFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

flex H2B GFP

Use

AAV

Tags

Expression

Mutation

Promoter

human Synapsin

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pRGE32

Plasmid

#63159

Purpose

Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for transient expression in protoplast

Depositor

Yinong Yang

Article

Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Rice snoRNA U3 promoter for gRNA/PTG expression a…

Available since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pLHCX-gpASNase1

Plasmid

#121526

Purpose

Expresses gpASNase1 in mammalian cells.

Depositor

Lucas Sullivan, Matthew Vander Heiden

Article

Sullivan et al Nat Cell Biol. 2018 Jul;20(7):782-788. d

Insert

gpASNase1

Use

Retroviral

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pSMPP-mCherry-mTrim21

Plasmid

#104971

Purpose

Lentiviral Transfer Plasmid with mCherry-tagged mouse Trim21

Depositor

Leo James

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

Tripartite Motif containing 21 (Trim21 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mutation

Promoter

SFFV

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pSP2-48-merTetO-EFS-BLaR

Plasmid

#118712

Purpose

Contains an optimized 48-mer TetO repeat for imaging of desired loci

Depositor

Huimin Zhao

Article

Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

Inserts

Blasticidin resistance gene
EFS-NS promoter

Use

Tags

Expression

Mammalian

Mutation

Promoter

EFS-NS

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CibN-dCas9-CibN

Plasmid

#60553

Purpose

Expresses CibN-dCas9-CibN in mammalian cells

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

dCas9-CibN

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

inactivating Cas9 mutations D10A and H840A

Promoter

CMV

Available since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCF806-shRen.713

Plasmid

#186712

Purpose

Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Non-targeting control shRNA (shRen.713).

Depositor

Christof Fellmann

Article

Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

Insert

shRen.713

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-TadA8e-Ns3h

Plasmid

#221227

Purpose

Express HACE Editor as a fusion of TadA-8e adenosine deaminase and Ns3h helicase.

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

TadA8e-Ns3h

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Cdc42(Q61L)

Plasmid

#12600

Depositor

Klaus Hahn

Article

Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

Insert

EGFP-Cdc42 (Q61L) (CDC42 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Q61L

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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ULK1-Halo HRD

Plasmid

#207548

Purpose

Homologous recombination donor to integrate a 3xFLAG-HaloTag at the C-terminus of the endogenous ULK1 locus in human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

HaloTag followed by a PolyA signal and PuroR cassette flanked by human ULK1 locus sequences

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Endogenous

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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