We narrowed to 15,780 results for: LEA

1. pcDNA3.1-MUC2 D1D2D'D3 with cleavable His tag

   Plasmid

   #187994

   Purpose

   Expresses MUC2 D1D2D'D3 assembly with cleavable His tag in mammalian cells

   Depositor

   Deborah Fass

   Article

   Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

   Insert

   mucin2 D1D2D'D3 (MUC2 Human)
   

   Use

   Tags

   6xHistadine followed by TEV cleavage site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3E-H2B-mCerulean3-P2A-mKOFP2-CAAX

   Plasmid

   #75177

   Purpose

   Nuclear mCerulean3 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with C-terminal fusions.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   H2B-mCerulean3-P2A-mKOFP2-CAAX
   

   Use

   Three prime entry vector for multisite gateway th…

   Tags

   Expression

   Mutation

   Promoter

   n/a

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. PEA1-Nuclease-Puro HEK3 CTT ins

   Plasmid

   #171995

   Purpose

   Delivers all prime editing nuclease components targeting the HEK3 gene for a CTT inserrtion, in a single, puromycin selectable plasmid

   Depositor

   Paul Thomas

   Article

   Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

   Insert

   HEK3 CTT insertion pegRNA and CbH-Cas9-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA sham
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV for Cas9, U6 for gRNAs

   Available since

   Sept. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-MUC2 D1D2D' with cleavable His tag

   Plasmid

   #187993

   Purpose

   Expresses MUC2 D1D2D' assembly with cleavable His tag in mammalian cells

   Depositor

   Deborah Fass

   Article

   Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

   Insert

   Mucin2 D1D2D' (MUC2 Human)
   

   Use

   Tags

   6xHistadine followed by TEV cleavage site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSBbi-Pur-mCerulean3-spacer-cpVenus[E172]

   Plasmid

   #125204

   Purpose

   SB-transposon plasmid for stable expression of mCerulean3-cpVenus[E172] FRET low control

   Depositor

   Eric Davis

   Article

   Havranek et al Blood. 2017 Aug 24;130(8):995-1006. doi:

   Insert

   mCerulean3-spacer-cpVenus[E172]
   

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Amber

   Plasmid

   #105806

   Purpose

   Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Amber, cleavable by TEV. Control for protein interaction analysis by FRET.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   ORF1: 3C-Cerulean; ORF2: TEV-Amber

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAB26 (punc-17::GtACR2::mCerulean::βHK::Chrimson)

   Plasmid

   #214889

   Purpose

   Expresses the optogenetic tool BiPOLES in cholinergic neurons of C. elegans.

   Depositor

   Alexander Gottschalk

   Article

   Bergs et al Nat Commun. 2023 Apr 6;14(1):1939. doi:

   Insert

   GtACR2_mCerulean_bHK_Chrimson
   

   Use

   Tags

   mCerulean

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAB27 (pmyo-3::GtACR2::mCerulean::βHK::Chrimson)

   Plasmid

   #214890

   Purpose

   Expresses the optogenetic tool BiPOLES in BWMs of C. elegans.

   Depositor

   Alexander Gottschalk

   Article

   Bergs et al Nat Commun. 2023 Apr 6;14(1):1939. doi:

   Insert

   GtACR2_mCerulean_bHK_Chrimson
   

   Use

   Tags

   mCerulean

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAB31 (punc-47::GtACR2::mCerulean::bHK::Chrimson)

   Plasmid

   #214891

   Purpose

   Expresses the optogenetic tool BiPOLES in GABAergic neurons of C. elegans.

   Depositor

   Alexander Gottschalk

   Article

   Bergs et al Nat Commun. 2023 Apr 6;14(1):1939. doi:

   Insert

   GtACR2_mCerulean_bHK_Chrimson
   

   Use

   Tags

   mCerulean

   Expression

   Mutation

   Promoter

   Available since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. EV-A71 2A protease; 6xHis-tag, self cleaving

    Plasmid

    #204809

    Purpose

    Bacterial expresison of Enterovirus A71 2A protease

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    A71 2A protease
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pSems-leader-SNAPf-mEGFPm-IFNAR1(28-557)

    Plasmid

    #192704

    Purpose

    Expression of SNAPf and mEGFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    IFNAR1 (IFNAR1 Human)
    

    Use

    Tags

    Ig k-chain leader sequence, SNAPf-tag, and meGFPm…

    Expression

    Mammalian

    Mutation

    meGFPm: gluatmic acid 142 to asparagine and histi…

    Promoter

    CMV

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSems-leader-SNAPf-mXFPm-IFNAR1(28-557)

    Plasmid

    #192784

    Purpose

    Expression of SNAPf and mXFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    IFNAR1 (IFNAR1 Human)
    

    Use

    Tags

    Ig k-chain leader sequence, SNAPf-tag, and mXFPm

    Expression

    Mammalian

    Mutation

    mXFPm: tryptophan 66 to phenylalanine, gluatmic a…

    Promoter

    CMV

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+)/(CAA)26 5′ leader GUG-nLuc-3XFLAG

    Plasmid

    #127325

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with (CAA)26 repeats in 5' UTR

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)/(CAA)26 5′ leader AUG-nLuc-3XFLAG

    Plasmid

    #127324

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with (CAA)26 repeats in 5' UTR

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJFRC81-3xVAS4-Syn21-FLAG-Cerulean-P10

    Plasmid

    #104618

    Purpose

    Expresses FLAG tagged Cerulean upon binding of TALE4 to VAS4

    Depositor

    Tudor Fulga

    Article

    Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

    Insert

    hsp70-Syn21-FLAG-mCerulean-P10
    

    Use

    Tags

    FLAG

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1_HA-p115-TEV-cleaved #957#382

    Plasmid

    #42107

    Depositor

    Ayano Satoh

    Article

    Koreishi et al Antibody Technology Journal. 2011;1:5-11

    Insert

    p115
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deleted 886 to end and added ENLYFQ

    Promoter

    CMV

    Available since

    Feb. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.1-Cerulean-Epac2(R447)-cpV[E172]

    Plasmid

    #138198

    Purpose

    FRET-based Epac2 activation sensor with R447A mutation in Epac2 coding sequence.

    Depositor

    Jin Zhang

    Article

    Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

    Insert

    Cerulean-Epac2(R447A)-cpVenus[E172] (Rapgef4 Mouse, Synthetic)
    

    Use

    Tags

    Cerulean and cpV[E172]

    Expression

    Mammalian

    Mutation

    Contains arginine-to-alanine substitution at resi…

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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18. PCDNA3-FlipGFP(Casp3 cleavage seq) T2A mCherry

    Plasmid

    #124428

    Purpose

    Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(Casp3 cleavage seq) T2A mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PCDNA3-FlipGFP(TEV cleavage seq) T2A mCherry

    Plasmid

    #124429

    Purpose

    Expresses FlipGFP (TEV cleavage sequence) and T2A mCherry in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(TEV cleavage seq) T2A mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PCDNA3-FlipGFP(Caspase-1 cleavage seq) T2A mCherry

    Plasmid

    #124494

    Purpose

    Expresses FlipGFP (caspase-1 cleavage sequence) and T2A mCherry

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(Caspase-1 cleavage seq) T2A mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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