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Plasmid#59827PurposeAllows the integration of myc Separase delta 55/Non-Cleavable in the genome and Tet-inducible expression.DepositorInsertSeparase (ESPL1 Human)
UseTagsMycExpressionMammalianMutationdelta 55/Non-Cleavable (E1483R, R1486E, E1503R, R…Promoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera1
Plasmid#79379PurposeCas9 gRNA variant to recruit ms2 proteinsDepositorInsertChimera 1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera4
Plasmid#79382PurposeCas9 gRNA variant to recruit ms2 proteinsDepositorInsertChimera 4
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean
Plasmid#24334DepositorInsertH2B
UseCre/LoxTagsGFPExpressionWormMutationmCherry driven by an HSP70 promoterCerulean drive…PromoterAvailable sinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
CA-0252 CMVTO-c-myc-mono-hIL10 (R5A11M)-rens-cleaved
Plasmid#231215PurposeCleaved-myc-IL-10DepositorInsertc-myc-mono-hIL10 (R5A11M)-rens-cleaved (IL10 Human)
UseTagsExpressionMammalianMutationN36I, N110I, K117N, F129LPromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
EV-A71 2A protease; 6xHis-MBP-TEV tag, self cleaving
Plasmid#204811PurposeBacterial expresison of Enterovirus A71 2A proteaseDepositorInsertA71 2A protease
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCR-II-TOPO-mitfa_LexPR-2A-Cerulean-SV40pA-FRT-Kan-FRT: Sequences,
Plasmid#110201PurposeMelanocyte-specific expression of the LexPR-2A-Cerulean fusionDepositorInsertLexPR transactivator - 2A - Cerulean
UseTagsCeruleanExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-H2B-iRFP670-p2a-mCerulean-Geminin (1–110)-IRES-Neomycin
Plasmid#223959PurposeDual fluorescent reporter for histone H2B and for APC/C activityDepositorUseLentiviralTagsiRFP670 and mCeruleanExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
SPOV strain SM-6 V-1 NS2B-NS3 protease fusion with non cleavable linker
Plasmid#228656PurposeBacterial expression of the fusion of the NS2B-NS3 protease from Spondweni virus for biochemical assayDepositorInsertSpondweni virus portion of NS2B protease fused to NS3
UseTagsHis-Twin Strep-TevExpressionBacterialMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
POWV strain LB NS2B-NS3 protease fusion with non cleavable linker
Plasmid#228654PurposeBacterial expression of the fusion of the NS2B-NS3 protease from Powassan virus for biochemical assayDepositorInsertPowassan virus portion of NS2B protease fused to NS3
UseTagsHis-Twin Strep-TevExpressionBacterialMutationAlanine mutant to stop self-cleaving ..VEREEA*AL.…PromoterAvailable sinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLV-H2B-iRFP670-p2a-mCerulean-Cdt1 (1-100)-IRES-puromycin
Plasmid#223965PurposeDual fluorescent reporter for histone H2B and for Cdt1DepositorUseLentiviralTagsiRFP670 and mCeruleanExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
WESSV strain SAH177 NS2B-NS3 protease fusion with non cleavable linker
Plasmid#228659PurposeBacterial expression of the fusion of the NS2B-NS3 protease from Wesselsbron virus for biochemical assayDepositorInsertWesselsbron virus portion of NS2B protease fused to NS3
UseTagsHis-Twin Strep-TevExpressionBacterialMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
SLEV strain Kern217 NS2B-NS3 protease fusion with non cleavable linker
Plasmid#228657PurposeBacterial expression of the fusion of the NS2B-NS3 protease from St. Louis encephalitis virus for biochemical assayDepositorInsertSLEV portion of NS2B protease fused to NS3
UseTagsHis-Twin Strep-TevExpressionBacterialMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
MVEV strain MVE-1-51 NS2B-NS3 protease fusion with non cleavable linker
Plasmid#228653PurposeBacterial expression of the fusion of the NS2B-NS3 protease from Murray Valley encephalitis virus for biochemical assayDepositorInsertMVEV portion of NS2B protease fused to NS3
UseTagsHis-Twin Strep-TevExpressionBacterialMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
DENV-4 strain H241 NS2B-NS3 protease fusion with non cleavable linker
Plasmid#228652PurposeBacterial expression of the fusion of the NS2B-NS3 protease from Dengue virus serotype 4 for biochemical assaysDepositorInsertDengue 4 portion of NS2B protease fused to NS3
UseTagsHis-Twin Strep-TevExpressionBacterialMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJFRC81-3xVAS1-Syn21-Citrine-HA-P10-3xVAS4-Syn21-FLAG-Cerulean-P10
Plasmid#104619PurposeExpresses HA tagged Citrine or FLAG tagged Cerulean upon binding of TALE1 to VAS1 or TALE4 to VAS4DepositorInsert3xVAS4-hsp70-Syn21-FLAG-Cerulean-P10
UseTagsFLAGExpressionInsectMutationPromoterhsp70Available sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
EV-A71 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tag
Plasmid#204816PurposeBacterial expresison of Enterovirus A71 3C proteaseDepositorInsertEV A71 3C protease
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
EV-D68 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tag
Plasmid#204817PurposeBacterial expresison of Enterovirus D68 3C proteaseDepositorInsertEV D68 3C protease
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pIRES-mCerulean-FRB-Cdc42/N12-Cdc42/13C(Q61L)-FKBP-mVenus-CAAX
Plasmid#214278PurposeExpress split-Cdc42 fragments that are fused with CIDs and FPsDepositorInsertsUseTagsmCerulean (on insert 1) and mVenus (on insert 2)ExpressionMammalianMutationCdc42-Q61LPromoterCMVAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTol2-Hsp70-H2B-mCerulean-lsl-mScarlet-fli1-zCre-I-BFP (JDW 1233)
Plasmid#229841PurposeA Tol2 based expression vector with the Hsp70 promoter driving a cre dependent switch reporter. Contains an endothelial driven creDepositorInsertloxp-H2B-mCerulean-2xStop-loxP
UseCre/Lox; Tol2 based expression vectorTagsExpressionMutationPromoterAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only