We narrowed to 18,943 results for: Tec

1. pGolden-Neo

   Plasmid

   #51422

   Purpose

   Contain one loxP-PGK-Neo-pA-loxP cassette, used for golden-gate reaction

   Depositor

   Yonglun Luo

   Article

   Luo et al Biotechniques. 2014 May 1;56(5):263-8. d

   Insert

   PGK1 phosphoglycerate kinase 1 (PGK1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. ML1Nmut_in_pEGFP-C1

   Plasmid

   #67796

   Purpose

   Mammalian expression vector driving Mouse ML1Nwt (2xML1N domain 7Q mutant version) fused to EGFP

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   ML1N*2(7Q)-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. Stx1a-12aa-mCer

   Plasmid

   #53236

   Purpose

   Fluorescent probe: Syntaxin 1A, C-terminally-tagged with monomeric Cerulean, connected with a 12 amino acid linker. Expressed in mammalian cells, used with SNAP25B-12aa-mCit to measure FRET & dispers.

   Depositor

   Robert Zucker

   Article

   Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

   Insert

   Syntaxin 1A (Stx1a Rat)
   

   Use

   Tags

   12aa-linker and mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

4. BFP Homology Donor

   Plasmid

   #149372

   Purpose

   Promoter-less BFP cDNA with two homology arms to the lenti-CDDR reporter for HDR detection

   Depositor

   Tarek Abbas

   Article

   Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

   Insert

   CDDR BFP Homology Donor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. P823_eSpCas9(1.1)-RecA

   Plasmid

   #87264

   Purpose

   Human codon optimized RecA protein was fused to eSpCas9(1.1) for enhanced genome editing efficiency

   Depositor

   Yonglun Luo

   Article

   Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

   Insert

   RecA
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. P628_SpCas9-RecA

   Plasmid

   #87263

   Purpose

   Human codon optimized RecA protein was fused to SpCas9 for enhanced genome editing efficiency

   Depositor

   Yonglun Luo

   Article

   Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

   Insert

   RecA
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   no

   Promoter

   CBh

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTE1062

   Plasmid

   #172356

   Purpose

   Detection of Streptomyces coelicolor butyrolactones (SCB) by kanamycin bioassay. Streptomyces genomic integrative vector.

   Depositor

   Eriko Takano

   Article

   Biarnes-Carrera et al Methods Mol Biol. 2018;1673:117-128. doi

   Inserts

   scbR (SCO6265 )
   kasOp
   

   Use

   Synthetic Biology; Integrative into streptomyces …

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXylA-agrCA-I

   Plasmid

   #53437

   Purpose

   xylose-dependent expression of the type-I agrC and agrA genes of S. aureus

   Depositor

   Cynthia Collins

   Article

   Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

   Insert

   agrCA locus
   

   Use

   Synthetic Biology

   Tags

   none

   Expression

   Bacterial

   Mutation

   "MVQTS" sequence added to the N-terminu…

   Promoter

   PxylA

   Available Since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGolden-Hyg

   Plasmid

   #51423

   Purpose

   Contain one loxP-PGK-Hyg-pA-loxP cassette, used for golden-gate reaction

   Depositor

   Yonglun Luo

   Article

   Luo et al Biotechniques. 2014 May 1;56(5):263-8. d

   Insert

   PGK-Hyg-pA (PGK1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSBL_DK187

    Plasmid

    #156423

    Purpose

    Insect cell expression of 3a-sfGFP, needs to be introduced into baculoviral bacmid

    Depositor

    Stephen Brohawn

    Article

    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

    Insert

    SARS-CoV-2 3a protein
    

    Use

    Tags

    sfGFP-His

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCellFree_G07

    Plasmid

    #67141

    Purpose

    Gateway destination vector for cell-free expression with N-term myc-tag mCherry tag

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

    Type

    Empty backbone

    Use

    Cell-free expression

    Tags

    His-MYC and mCherry

    Expression

    Mutation

    Promoter

    Available Since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT7-agrBD-I

    Plasmid

    #53439

    Purpose

    T7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220

    Depositor

    Cynthia Collins

    Article

    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

    Insert

    agrBD locus
    

    Use

    Synthetic Biology

    Tags

    V5 epitope, 6x HIS (not expressed on agrB or agrD…

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7-lac

    Available Since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCellFree_G08

    Plasmid

    #67142

    Purpose

    Gateway destination vector for cell-free expression with C-term mCherry myc-tag tag

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

    Type

    Empty backbone

    Use

    Cell-free expression

    Tags

    His-MYC and mCherry

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pACYC-Skp-hLYZ

    Plasmid

    #83924

    Purpose

    Co-expresses cytoplasmic copies of the Skp protein folding chaperone and wild type human lysozyme..

    Depositor

    Karl Griswold

    Article

    Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

    Inserts

    Skp
    lysozyme (LYZ Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pXylA-agrCA-IV

    Plasmid

    #53438

    Purpose

    xylose-dependent expression of the type-IV agrC and type-I agrA genes of S. aureus

    Depositor

    Cynthia Collins

    Article

    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

    Insert

    agrCA locus
    

    Use

    Synthetic Biology

    Tags

    none

    Expression

    Bacterial

    Mutation

    "MVQTS" sequence added to the N-terminu…

    Promoter

    PxylA

    Available Since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Cav1-miniSOG

    Plasmid

    #67667

    Purpose

    Mammalian expression vector driving dog Caveolin1-minSOG

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    Dog Cav1-miniSOG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HSA_3b*_AP

    Plasmid

    #238899

    Purpose

    Plasmid insert contains a gene sequence for Human serum albumin for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    Recombinant Human Serum Albumin (HSA) 3b*
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. C-term_TEV_aMFx1 (4a)

    Plasmid

    #238903

    Purpose

    Plasmid insert contains the aMFx1 C-terminal tag sequence with the TEV cleavage site for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    C-term_TEV_aMFx1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. C-term_C-myc (4a)

    Plasmid

    #238907

    Purpose

    Plasmid insert contains the C-myc C-terminal tag sequence for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    C-term_C-myc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGS-SAP30

    Plasmid

    #109212

    Purpose

    Encodes human full-length SAP30 to be expressed in a baculovirus/insect cell expression system

    Depositor

    Monika Golas

    Article

    Jayaprakash et al Mol Biotechnol. 2021 Jan;63(1):53-62. do

    Insert

    Human full-length SAP30 (SAP30 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    p10

    Available Since

    July 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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