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pLVX-EGFP-IRES-Neo

Plasmid

#128660

Purpose

mammalian expression of EGFP with neomycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

EGFP

Use

Lentiviral

Tags

N/A

Expression

Mammalian

Mutation

Wild type

Promoter

CMV

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLdCN

Plasmid

#84290

Purpose

Express gRNA and Cas9 in Leishmania with Neomycin resistance

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

Insert

gRNA and Cas9

Use

CRISPR; Leishmania

Promoter

L. donovani ribosome RNA promoter

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Neo

Plasmid

#139449

Purpose

Lentiviral vector with gRNA scaffold and neomycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

no sgRNA inserted; resistance gene: neoR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pLCN DSB Repair Reporter (DRR)

Plasmid

#98895

Purpose

DSB Repair Reporter (DRR). Expresses GFP after cut of two inverted ISce1 sites and repair by NHEJ.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

Neomycin-T2A-ISce1-ISce1-eGFP

Use

Lentiviral

Promoter

CMV

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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EFS-EGFP-P2A-Neo-WPRE-U6-AsCas12aDR-AsCas12aDR (pRG212)

Plasmid

#149722

Purpose

Lentiviral expression of crRNA with GFP and a neomycin resistance gene

Depositor

Junwei Shi

Article

Gier et al Nat Commun. 2020 Jul 13;11(1):3455. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter for crRNA expression and EFS promoter…

Available Since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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pSLIK-Neo

Plasmid

#25735

Purpose

3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Neomycin resistance gene coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo_LKB1

Plasmid

#108111

Purpose

Lentiviral expression plasmid of human LKB1 cDNA with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Insert

LKB1 (STK11 Human)

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS promoter

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pZCS38 (Prsp-27::NeoR::unc-54 3’ UTR)

Plasmid

#193049

Purpose

Encodes G-418 resistance

Depositor

Patrick Phillips

Article

Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

Insert

prsp-27::NeoR::unc-54 3’ UTR

Expression

Worm

Promoter

prsp-27

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo_SIK3

Plasmid

#108103

Purpose

Lentiviral expression plasmid of human SIK3 cDNA with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Insert

SIK3 (SIK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EFS promoter

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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KL063 - pCAGGS-OsTIR1(F74G)-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26

Plasmid

#232929

Purpose

Targeting vector to introduce an OsTir1(F74G) expressing cassette at the mouse Rosa26 locus using Neomycin selection. AID2 degron system. Use with Addgene #64216 sgRNA.

Depositor

Danny Reinberg

Article

Lucero et al bioRxiv, March 19, 2025

Insert

OsTir1(F74G)

Use

Mouse Targeting

Expression

Mammalian

Mutation

changed Phenylalanine 74 to Glycine

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-P1-LoxP-Neo-N-miRFP670-C

Plasmid

#159434

Purpose

CRISPR knock-in donor construct with fluorescent reporter and removable selectable marker

Depositor

Jianhua Xing

Article

Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

Inserts

Neomycin
miRFP670

Use

CRISPR

Tags

3' linker pair 1, 5' linker pair 1, C t…

Promoter

SV40

Available Since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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PB-tight-DDdCas9GFP-IRES-Neo

Plasmid

#102907

Purpose

PiggyBac construct with doxycyline inducible TRE-tight promoter expression of dCas9-GFP followed by IRES-Neomycin selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

DDdCas9GFP-IRES-Neo

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

TRE-tight

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-Neo-PuroDTK

Plasmid

#84036

Purpose

PiggyBac cassette contains Neomycin, puromycin and TK genes

Depositor

Yuet Wai Kan

Article

Ye et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

Type

Empty backbone

Expression

Bacterial and Mammalian

Promoter

CAG promoter

Available Since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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PB-EF1-IRES-NEO deleted ITR

Plasmid

#67906

Purpose

PiggyBac transposon encoding Neomycin resistance with deleted inverted terminal repeat

Depositor

Alex Kentsis

Article

Henssen et al Elife. 2015 Sep 25;4. pii: e10565. doi:

Insert

NeoR (neoR Synthetic)

Expression

Mammalian

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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PB-EF1-IRES-NEO mutant ITR

Plasmid

#67905

Purpose

PiggyBac transposon encoding Neomycin resistance with inverted terminal repeat mutation

Depositor

Alex Kentsis

Article

Henssen et al Elife. 2015 Sep 25;4. pii: e10565. doi:

Insert

NeoR (neoR Synthetic)

Expression

Mammalian

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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Nanog-luciferase targeting construct

Plasmid

#26591

Depositor

Rudolf Jaenisch

Article

Lyssiotis et al Proc Natl Acad Sci U S A. 2009 Jun 2. 10

Insert

Nanog promoter-luciferase-lox-pgk-neomycin-loc

Expression

Bacterial

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo_SIK3_T142Q

Plasmid

#108110

Purpose

Lentiviral expression plasmid of human SIK3 cDNA (gatekeeper mutation) with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Insert

SIK3 (SIK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

change Threonine 142 to Glutamine

Promoter

EFS promoter

Available Since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pBK-EcTrpRS(H14)

Plasmid

#231129

Purpose

Expresses mutant E. coli TrpRS from a weak glnS promoter for proteome-wide incorporation of tryptophan analogues.

Depositor

Abhishek Chatterjee

Article

Loynd et al Nat Chem Biol. 2025 Oct 2. doi: 10.1038/

Insert

E. coli tryptophanyl tRNA synthetase

Expression

Bacterial

Mutation

S8A, V144G, V146C

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pBBR1-EcTrpRS(H14)

Plasmid

#231131

Purpose

Expresses mutant E. coli TrpRS from a derepressed lac promoter for proteome-wide incorporation of tryptophan analogues in E. coli/K. pneumoniae.

Depositor

Abhishek Chatterjee

Article

Loynd et al Nat Chem Biol. 2025 Oct 2. doi: 10.1038/

Insert

E. coli tryptophanyl tRNA synthetase

Use

Klebsiella pneumoniae expression

Expression

Bacterial

Mutation

S8A, V144G, V146C

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pSGAb/pEvol-AbTrpRS(H14)

Plasmid

#231133

Purpose

Expresses mutant A. baumannii TrpRS from an inducible lac promoter for proteome-wide incorporation of tryptophan analogues.

Depositor

Abhishek Chatterjee

Article

Loynd et al Nat Chem Biol. 2025 Oct 2. doi: 10.1038/

Insert

A. baumannii tryptophanyl tRNA synthetase

Use

Acinetobacter baumannii expression

Expression

Bacterial

Mutation

T12A, V151G, V153C

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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