We narrowed to 19,597 results for: tec
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Plasmid#194088PurposeExpresses Ec86 reverse transcriptase in Escherichia coliDepositorInsertEc86 rrt
TagsHis8PromoterT7Available SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPMVAd36
Plasmid#98302PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or CUP1 promoter.DepositorInsertPCUP1>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1ExpressionYeastAvailable SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
piGECI-NES-N1
Plasmid#160422PurposeExpression of a near-infrared genetically encoded calcium indicator iGECI with a nuclear export signal sequenceDepositorInsertiGECI-NES
ExpressionMammalianPromoterCMVAvailable SinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEAST-BenR
Plasmid#114597Purpose"Strong constitutive expression of transcription factor, BenR, for cell-free expression"DepositorInsertBenR
UseSynthetic Biology; Cell-free protein synthesisPromoterOR2-OR1-PrAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-ExRai-AKAR(T/A)
Plasmid#118406PurposeNegative-control mutant for ExRai-AKAR biosensor.DepositorInsertExRai-AKAR(T/A)
Tags6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable SinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSI-535
Plasmid#131130PurposegRNA expression vector for C-to-T base editing reporterDepositorInsertC-to-T reporter activation gRNA
UseCRISPRExpressionMammalianPromoterHuman U6 promoterAvailable SinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSP2279
Plasmid#70706PurposeBacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2DepositorInsertsmammalian codon-optimized Staphylococcus aureus dCas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationD10A and H557A in SaCas9PromoterT7Available SinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
AA345
Plasmid#216031PurposeFragmid fragment: (Cas protein) Cas13dDepositorHas ServiceCloning Grade DNAInsertCas13d_v1.1 [Dj]
UseCRISPR; FragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
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p2CT-His-MBP-Lbu_C2c2_R472A_H477A
Plasmid#83483PurposeBacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 1 inactive mutant).DepositorInsertLbu_C2c2_R472A_H477A
UseCRISPRTagsHis6-MBPExpressionBacterialMutationR472A, H477AAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB528
Plasmid#74870PurposeGateway cloning compatible binary vector for C-terminal fusion with TAP (no promoter).DepositorTypeEmpty backboneExpressionPlantAvailable SinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-sapphireAKAR
Plasmid#118466Purposecp-T-sapphire-based single-color PKA biosensor targeted to the plasma membrane.DepositorInsertLyn-sapphireAKAR
TagsN-terminal targeting sequence from Lyn kinaseExpressionMammalianPromoterCMVAvailable SinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
A3A-PBE-ΔUGI
Plasmid#119770PurposeConversion of C to T in wheat plants or monocotyledons protoplasts without uracil glycosylase inhibitor (UGI)DepositorInsertAPOBEC3A-nCas9-ΔUGI-NLS
TagsNLSExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9Promotermaize Ubiquitin-1Available SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-sapphireAKAR(T/A)
Plasmid#118465PurposeNegative-control mutant for sapphireAKAR biosensor.DepositorInsertsapphireAKAR(T/A)
Tags6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable SinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC57mini ZM249M P90A _env eGFP
Plasmid#132939PurposepUC57mini ZM249M _env eGFP containing P90A mutant capsid sequenceDepositorInsertHIV-1ZM249M provirus (P90A) with env deletion, GFP
UseLentiviralMutationP90AAvailable SinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDC184
Plasmid#70055PurposeCo-expresses Syt1-SNARE37aa-linker complex with p045DepositorInsertsHis tagged Synaptobrevin 28-89
Syntaxin 191-256
Tags10xHisExpressionBacterialPromoterT7Available SinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
RFN_EGFP_81
Plasmid#60073PurposeExpresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nucleaseDepositorInsertpair of multiplex gRNAs targeting EGFP
UseCRISPRExpressionMammalianPromoterU6Available SinceOct. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pCfB3034(X-3 MarkerFree)
Plasmid#73272PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-3 (Chr X: 223616..224744)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMXSIP-GPR35-mCherry
Plasmid#204711Purposeexpresses GPR35-mCherry in mammalian cellsDepositorAvailable SinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only