We narrowed to 10,315 results for: otos
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Plasmid#21688DepositorInsertGolgi Phosphoprotein 3 (GOLPH3 Human)
UseGateway entry cloneMutationlast nucleotide of stop codon removed for c-termi…Available SinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-CCre-MBP1-WPRE
Plasmid#193917PurposeExpresses one of the components of Cre-DOR_N6C1 (RFP-dependent Cre)DepositorInsertCCre-MBP1
UseAAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…ExpressionMammalianPromoterEF1aAvailable SinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-NCre-MBP6-WPRE
Plasmid#193916PurposeExpresses one of the components of Cre-DOR_N6C1 (RFP-dependent Cre)DepositorInsertNCre-MBP6
UseAAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…ExpressionMammalianPromoterEF1aAvailable SinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-Ubc9-C138A
Plasmid#133911PurposeBacterial expression of His6-Ubc9-C138ADepositorInsertSUMO-conjugating enzyme UBC9 (UBE2I Human)
TagsThrombin-cleavable His6-tagExpressionBacterialMutationCysteine 138 to AlaninePromoterT7Available SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSN672
Plasmid#177362PurposeExpresses human KIF1A(1-393) fused with leucine zipper and His tagDepositorAvailable SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
ApoE2_mCh-SspB (pBS1142)
Plasmid#185324PurposeFor the mammalian expression of the human protein ApoE2 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formationDepositorInsertApoE2
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_BB_2A-GFP_MAPRE3-gRNA#3
Plasmid#107731PurposeKnock out of EB3 in human cells by CRISPR/Cas9DepositorAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PTBP1-RRM12CCtoSS
Plasmid#89155PurposeRecombinant expression of PTBP1-RRM12 C250S,C251S in E.coliDepositorInsertPolypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1+2 mutant C250S, C251S (PTBP1 Human)
Tagshexa HisExpressionBacterialMutationmutations: Cys 250 to Ser, Cys 251 to SerPromoterT7Available SinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pECE-M2-BAIAP2 S366A
Plasmid#31657DepositorInsertBAI1-associated protein 2 S366A (BAIAP2 Human)
TagsM2ExpressionMammalianMutationchanged Serine 366 to AlanineAvailable SinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-pax3a-kcnj13-IRES-EGFP
Plasmid#164950PurposeTol2 construct: pax3a-5.4k promoter driven zebrafish kcnj13 fused with EGFP and EGFPDepositorInsertkcnj13 (kcnj13 Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPPromoterpax3a promoter -5.4kAvailable SinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcnj13-IRES-EGFP
Plasmid#164941PurposeTol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 and EGFPDepositorInsertkcnj13 (kcnj13 Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPPromoteractinb (actb2) promoterAvailable SinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFrt-invCAG-Luc
Plasmid#63577PurposeShuttle vector compatible with Flp-mediated (Flp-in) transgene integration that allows for conditional Luciferase expression upon integration. The vector contains an FRT site and two mutant loxP sitesDepositorInsertStop-Lox71-Luciferase-pA-FRT-Lox66-reverseCAG
UseCre/Lox; Frt/flpe recombination mediated insertionExpressionMammalianAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAN19-3xFLAG-TIR1-NOSt
Plasmid#108545PurposeCloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 [Wild type] and NOS terminatorDepositorInsertTRANSPORT INHIBITOR RESPONSE 1 fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)
UseCloning vectorTags3xFLAGAvailable SinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
p28BIOHTEV-LIC: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2
Plasmid#167848PurposeBacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNADepositorInsertnsp10, nsp16
Tags6xHis and AviTagExpressionBacterialPromoterT7Available SinceApril 22, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHR-mCherry-p2A-LIC1 G domain
Plasmid#74602Purposeexpresses LIC1 in mammalian cells; aa 1-389, untagged but cells indicate expression with diffuse mCherryDepositorInsertfull length human dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)
UseLentiviralTagsHA tagExpressionMammalianPromoterSFFVAvailable SinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pECE beta1 R760A
Plasmid#50511DepositorAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-REX-GECO0.9
Plasmid#61249PurposeExpresses REX-GECO0.9 in neuronsDepositorInsertREX-GECO0.9
UseAAVExpressionMammalianMutationSubstitutions relative to R-GECO1: P60R, V61W, R6…PromoterhSynAvailable SinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail
Plasmid#60392PurposeExpression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) using a GAL promoterDepositorInsertTUB1 (TUB1 Budding Yeast, Synthetic)
ExpressionYeastMutationinternal 6xHis (see supplement figure 4), amino a…PromoterGALAvailable SinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
I-GGTT
Plasmid#43138DepositorInsertI-GGTT
UseBacterial cloningAvailable SinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
II-CTCG
Plasmid#43337DepositorInsertII-CTCG
UseBacterial cloningAvailable SinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only