We narrowed to 10,238 results for: ada

1. pmCherry-AHPH

   Plasmid

   #187278

   Purpose

   Express mCherry-AHPH (RhoA biosensor)

   Depositor

   Alpha Yap

   Article

   Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

   Insert

   Anillin and Pleckstrin homology domain (ANLN Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   AHPH

   Promoter

   CMV

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. IL2-mCherry in pmCherryN1

   Plasmid

   #187283

   Purpose

   Express IL2-mCherry

   Depositor

   Alpha Yap

   Article

   Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

   Insert

   interleukin-2 receptor alpha-subunit (IL2 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Str-KDEL_SBP-EGFP-LAP

   Plasmid

   #222322

   Purpose

   Synchronize the trafficking of LAP from the ER.

   Depositor

   Franck Perez

   Article

   Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

   Insert

   Streptavidin-KDEL and LAP fused to SBP-EGFP (ACP2 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Str-KDEL_SBP-EGFP-EGFR

   Plasmid

   #222325

   Purpose

   Synchronize the trafficking of EGFR from the ER.

   Depositor

   Franck Perez

   Article

   Scharaw et al J Cell Biol. 2016 Nov 21;215(4):543-558.

   Insert

   Streptavidin-KDEL and EGFR fused to SBP-EGFP (EGFR Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pT5_TRE-ARID1A-FLAG_PGK-H2B-RFP

   Plasmid

   #203965

   Purpose

   Dox inducible FLAG tag ARID1a in a sleeping beauty transposon construct

   Depositor

   Elaine Fuchs

   Article

   Yang et al Nat Cell Biol. 2023 Jul 24. doi: 10.1038

   Insert

   ARID1A (Arid1a Mouse)
   

   Use

   Sleeping beauty transposon

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-F-box:gs10:FRB:HA

   Plasmid

   #228531

   Purpose

   F-box domain of human Skp2 protein in fusion with FRB domain through a flexible glycine-serine linker.

   Depositor

   Roman Jerala

   Article

   Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

   Insert

   F-box:gs10:FRB:HA (SKP2 Human, Synthetic)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-myc:Rbx1:gs10:FRB:HA

   Plasmid

   #228534

   Purpose

   Human Rbx1 protein in fusion with FRB domain through a flexible glycine-serine linker.

   Depositor

   Roman Jerala

   Article

   Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

   Insert

   myc:Rbx1:gs10:FRB:HA (RBX1 Human, Synthetic)
   

   Tags

   HA and myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. eft-3p:act-5(ptc):tbb2 3' UTR

   Plasmid

   #202331

   Purpose

   The ubiquitous eft-3 promoter drives overexpression of the mutant act-5(ptc) gene including introns.

   Depositor

   Didier Stainier

   Article

   Welker et al PLoS Genet. 2023 Jun 29;19(6):e1010806.

   Inserts

   eft-3 promoter
   act-5(ptc)
   tbb-2 3' UTR
   

   Tags

   base 238 C -> A mutation causing a PTC at codo…

   Expression

   Worm

   Promoter

   This is a promoter sequence for C. elegans eft-3,…

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. qTAG-C-Solo-mScarlet-MAPRE1

   Plasmid

   #227323

   Purpose

   Donor template for mScarlet insertion into the C-terminus of the MAPRE1 locus. For growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 (Addgene #207793)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   MAPRE1 Homology Arms flanking a mScarlet Tag (MAPRE1 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. qTAG-C-Solo-mStayGold-EZR

    Plasmid

    #227294

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the EZR locus. For membrane visualization. To be co-transfected with sgRNA plasmid px330-EZR (Addgene #227293)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    EZR Homology Arms flanking a mStayGold Tag (EZR Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. qTAG-C-Solo-mStayGold-H3C2

    Plasmid

    #227334

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the H3C2 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 (Addgene #207780)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H3C2 Homology Arms flanking a mStayGold Tag (H3C2 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCHC030 (∆CBBp ∆MBH ∆SH)

    Plasmid

    #226205

    Purpose

    Electroporation knockout plasmid (pK18sB) to delete the CBBp, membrane-bound hydrogenase, soluble hydrogenase, and intervening sequences (∆CBBp ∆MBH ∆SH).

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for CBBp, MBH, and SH operons, and intervening sequencessequences.
    

    Use

    Synthetic Biology

    Mutation

    ∆cbbR ∆cbbLSXYEFPTZGKA ∆hoxKGZMLOQRTV ∆hypA1B1F1C…

    Available Since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. qTAG-C-Solo-miRFP670nano3-H3C2

    Plasmid

    #227335

    Purpose

    Donor template for miRFP670nano3 insertion into the C-terminus of the H3C2 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 (Addgene #207780)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H3C2 Homology Arms flanking a miRFP670nano3 Tag (H3C2 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. qTAG-N-Solo-mStayGold-MAP4

    Plasmid

    #227296

    Purpose

    Donor template for mStayGold insertion into the N-terminus of the MAP4 locus. For microtubule visualization. To be co-transfected with sgRNAplasmid px330-PITCh-MAP4 (Addgene #227295)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    MAP4 Homology Arms flanking a mStayGold Tag (MAP4 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. qTAG-C-Solo-mStayGold-CEP192

    Plasmid

    #227289

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the CEP192 locus. For pericentriolar material visualization. To be co-transfected with sgRNA plasmid px330-PITCh-CEP192 (Addgene #227288)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    CEP192 Homology Arms flanking a mStayGold Tag (CEP192 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSuper DGK zeta human

    Plasmid

    #224575

    Purpose

    To downregulate the expression of human DGK zeta in human cells

    Depositor

    Isabel Mérida

    Article

    Avila-Flores et al Immunol Cell Biol. 2017 Jul;95(6):549-56

    Insert

    sh RNAi Diacylglycerol kinase zeta human (DGKZ Human)
    

    Use

    RNAi

    Promoter

    H1

    Available Since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSuper Retro GFP DGK alfa mouse

    Plasmid

    #224576

    Purpose

    Negative Control for downregulation of the expression of human DGK alfa in human cells

    Depositor

    Isabel Mérida

    Article

    Avila-Flores et al Immunol Cell Biol. 2017 Jul;95(6):549-56

    Insert

    sh RNAi Diacylglycerol kinase alfa mouse (Dgka Mouse)
    

    Use

    RNAi and Retroviral

    Promoter

    H1

    Available Since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV-CMV-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

    Plasmid

    #177365

    Purpose

    AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Inserts

    tdTomato
    Chimeric guide for SaCas9
    

    Use

    AAV

    Tags

    P2A and a tetracycline-controlled transactivator …

    Expression

    Mammalian

    Promoter

    CMV and U6 promoter

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-MCHpr-DREADD (Gq)-mCherry

    Plasmid

    #221036

    Purpose

    Expresses Gq coupled DREADD hM3D under the rat pro-melanin-concentrating hormone promoter

    Depositor

    Yanhua Huang

    Article

    Wang et al Mol Psychiatry. 2021 Jul;26(7):3152-3168

    Insert

    DREADD Gq-mCherry
    

    Use

    AAV; Rat targeting

    Expression

    Mammalian

    Promoter

    Rat MCH

    Available Since

    Aug. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX330_DAZL_cterm2

    Plasmid

    #222919

    Purpose

    Cas9/sgRNA plasmid for targeting DAZL

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    Cas9, DAZL sgRNA 2 (DAZL Human, Synthetic)
    

    Use

    CRISPR

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge