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pGL0_3 [RSF1010 ORI]

Plasmid

#198920

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

RSF1010 ORI

Use

Synthetic Biology

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pDAS12222_U6-pegRNA-BFP

Plasmid

#177181

Purpose

pegRNA cloning plasmid with BFP marker

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pHO4d-Cas9

Plasmid

#67881

Purpose

Cas9nlsHis6 Bacterial Expression construct

Depositor

Michael Nonet

Article

Fu et al Nucleic Acids Res. 2014 Dec 16;42(22):13

Insert

Cas9

Use

CRISPR

Tags

His6 and T7 epitope

Expression

Bacterial

Promoter

T7

Available Since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pInt-ts

Plasmid

#66076

Purpose

The pInt-ts plasmid [Haldimann2001] is the lambda helper plasmid necessary for the genome integration of the burden monitor lambda version

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

Insert

lambda integrase

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only

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pCHW91070

Plasmid

#222463

Purpose

Level 1 acceptor, Position 3 containing CBS4-CMV1::mCherry2-HyP5SM-LacZ::nosT.

Depositor

Chih-Hang Wu

Article

Chiang et al bioRxiv 2024.03.23.586378

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Mutation

no

Promoter

CBS4-CMV1 (Cucumber Mosaic Virus)

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pGL0_9 [pUB110 ORI]

Plasmid

#198924

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pUB110 ORI

Use

Synthetic Biology

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pET23a-mUbc9

Plasmid

#53137

Purpose

E.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)

Depositor

Frauke Melchior

Article

Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Insert

Ubc9 (Ube2i Mouse)

Tags

untagged

Expression

Bacterial

Mutation

protein sequence is identical to human

Promoter

T7

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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XLone-Cre

Plasmid

#235216

Purpose

Tet-On 3G-inducible PiggyBac system for expression of Cre in human pluripotent stem cells.

Depositor

Xiaojun Lian

Article

Howells et al Biotechnol Bioeng. 2025 Aug 20. doi: 10.

Insert

Cre

Use

Cre/Lox

Expression

Mammalian

Available Since

Aug. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-Venus

Plasmid

#96940

Purpose

Retroviral plasmid containing Venus for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Venus Fluorescent Protein

Expression

Mammalian

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCRISPR::rpsL

Plasmid

#44505

Purpose

A crRNA expression plasmid specific to the rpsL allele.

Depositor

Luciano Marraffini

Article

Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

CRISPR::rpsL (rpsL )

Use

CRISPR; E.coli

Available Since

April 16, 2013

Availability

Academic Institutions and Nonprofits only

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pLCP FLAG B55 alpha

Plasmid

#13804

Depositor

William Hahn

Article

Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Insert

PP2A B55 alpha Flag (PPP2R2A Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

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pBABE zeo PPP2CA Wt

Plasmid

#10689

Depositor

William Hahn

Article

Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Insert

protein phosphatase 2, catalytic subunit, alpha isozyme (PPP2CA Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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pEM705 Gs15

Plasmid

#109360

Purpose

GsX chimera with Gα15 C-terminus

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gs15 chimera

Tags

None

Expression

Mammalian

Promoter

CAG

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pBad33_ISYmu1_reRNA

Plasmid

#205358

Purpose

Bacterial expression of ISYmu1 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISYmu1 TnpB

Expression

Bacterial

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pEE515

Plasmid

#149288

Purpose

T-DNA encoding TRV2 complete genome with MCS

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Type

Empty backbone

Expression

Plant

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pPSint

Plasmid

#149421

Purpose

Plasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted into the IV2 intron in the GFP reporter gene.

Depositor

Stan Fields

Article

Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

Type

Empty backbone

Expression

Plant

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCTCON2-Aga1p

Plasmid

#158141

Purpose

Expresses Aga1p in yeast

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

Aga1p

Expression

Yeast

Promoter

GAL10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-rev-erbα

Plasmid

#186823

Purpose

Fluorescent reporter for rev-erbα expression

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

rev-erbα promoter

Use

Luciferase

Promoter

rev-erbα promoter

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRsetB-RoTq-On

Plasmid

#234465

Purpose

Bacterial expression vector for RoTq-On

Depositor

Gary Yellen

Article

Rosen et al Proc Natl Acad Sci U S A. 2025 May 13;12

Insert

RoTq-On

Tags

7xHis

Expression

Bacterial

Available Since

May 16, 2025

Availability

Academic Institutions and Nonprofits only

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pET-15b-UbcH5b/2419

Plasmid

#11475

Depositor

Allan Weissman

Article

Jensen et al J Biol Chem. 1995 Dec 22. 270(51):30408-

Insert

UbcH5b (UBE2D2 Human)

Expression

Bacterial

Mutation

n/a

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only