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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 23,398 results for: cas9

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Showing: 6461 - 6480 of 23398 results

  2. pxCas9CR4

    Plasmid
    #111656
    Purpose
    PCas9CR4 with the xCas9 mutations allowing NG PAM sites
    Depositor
    Christopher Reisch
    Insert
    TetR and xCas9
    Use
    Synthetic Biology
    Tags
    ssrA
    Expression
    Bacterial
    Mutation
    xCas9 mutations
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111656-plasmid-map-sequence-id-217189

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  3. pX551-CMV-CjCas9

    Plasmid
    #107035
    Purpose
    AAV plasmid expressing CjCas9 under control of CMV promoter
    Depositor
    Alex Hewitt
    Insert
    CjCas9
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    107035-plasmid-map-sequence-id-208555

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  4. pAC147-pCR8-dCas9VP64

    Plasmid
    #48219
    Purpose
    dCas9VP64 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression 
    Depositor
    Rudolf Jaenisch
    Publication
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9(D10A;H840A) fusion with VP64 activation domain
    Use
    CRISPR and Gateway Cloning
    Tags
    HA Tag and VP64
    Mutation
    D10A;H840A
    Available since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48219-plasmid-map-sequence-id-69504

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  8. VPR_dCas9

    Plasmid
    #134601
    Purpose
    Express CHO codon-optimized dCas9 fused to VPR for CRISPR activation. 2A linked ZsGreen1 marker
    Depositor
    Lasse Ebdrup Pedersen
    Publication
    Karottki et al Biotechnol Bioeng. 2020 Feb;117(2):593-5
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VPR-dCas9-2A-ZsGreen-DR
    Tags
    VPR and ZsGreen1-DR
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    134601-plasmid-map-sequence-id-269007

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  10. CBh-dCas9-Mll3SET

    Plasmid
    #108452
    Purpose
    Expression vector of CRISPR/dCas9 fused with SET domain of mouse MLL3 gene, driven by CBh promoter
    Depositor
    Bing Ren
    Publication
    Yan et al Cell Res. 2018 Jan 9. pii: cr20181. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9, MLL3 (Kmt2c Mouse)
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    April 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    108452-plasmid-map-sequence-id-204629

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  13. pTRE3G-VP64-dCas9-VP64-ZsG

    Plasmid
    #192668
    Purpose
    Dox-inducible expression of VP64-dCas9-VP64 with 2A ZsGreen1
    Depositor
    Jeffrey Miner
    Insert
    VP64-dCas9-VP64, ZsGreen1
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    TRE3G
    Available since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192668-plasmid-map-sequence-id-379892

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  15. PB-TAC-OKMS-Cas9

    Plasmid
    #120353
    Purpose
    piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.
    Depositor
    Knut Woltjen
    Publication
    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    OKMS cassette
    Use
    CRISPR; Piggybac transposon
    Expression
    Mammalian
    Mutation
    Klf4 [10-483]
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    120353-plasmid-map-sequence-id-236054

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  18. pUF1-dCas9-GCN5

    Plasmid
    #122509
    Purpose
    Combining with the specific sgRNA sequence to activate of the Plasmodium falciparum endogenous gene transcription, through the increasing of H3K9 acetylation.
    Depositor
    Lubin Jiang
    Publication
    Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-GCN5
    Use
    CRISPR
    Tags
    3*FLAG
    Mutation
    dCas9(D10A,H840A)
    Promoter
    PfHsp86
    Available since
    June 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    122509-plasmid-map-sequence-id-245888

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Showing: 6461 - 6480 of 23398 results