228,568 results

1. pET mCherry LIC cloning vector (u-mCherry)

   Plasmid

   #29769

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)

   Plasmid

   #185911

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   human codon optimized ABE8e-nSpG-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEFIRES-P-EGFP-ADRP

   Plasmid

   #87157

   Purpose

   Fluorescent protein localized to lipid droplets (LDs)

   Depositor

   Elina Ikonen

   Article

   Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

   Insert

   ADRP (PLIN2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   mCherry-R TTGGTCACCTTCAGCTTGG

   Available since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLminP_Luc2P_RE1

   Plasmid

   #90335

   Purpose

   NFkB - p50/p65 pathway gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   NFKB

   Available since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-EGFP (AAV8)

   Viral Prep

   #50465-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Promoter

   Syn

   Tags

   EGFP

   Available since

   Oct. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3.1-NINJ1

   Plasmid

   #201180

   Purpose

   Transient expression of NINJ1 gene in mammalian cells

   Depositor

   Jonathan Kagan

   Article

   Borges et al Elife. 2022 Dec 5;11:e78609. doi: 10.755

   Insert

   Ninjurin 1 (Ninj1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE (AAV1)

   Viral Prep

   #130998-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE (#130998). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE plasmid DNA. Ef1a-driven, Cre-dependent expression of ChRmine-mScarlet. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Promoter

   EF1a

   Tags

   mScarlet

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

8. pQE-(NSP12)-pcI-ts,ind+-(NSP7-NSP8)

   Plasmid

   #160540

   Purpose

   Expresses codon optimized SARS-CoV-2 NSP7, NSP8, and NSP12 in E. coli.

   Depositor

   Kenneth Johnson

   Article

   Dangerfield et al iScience. 2020 Nov 28:101849. doi: 10.10

   Inserts

   SARS-CoV-2 NSP7 (ORF1ab Synthetic)
   SARS-CoV-2 NSP8 (ORF1ab Synthetic)
   SARS-CoV-2 NSP12 (ORF1ab Synthetic)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 10, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. ABE8.8-m

   Plasmid

   #136294

   Purpose

   expresses ABE8.8-m in mammalian cells

   Depositor

   Nicole Gaudelli

   Article

   Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

   Insert

   ABE8.8-m
   

   Use

   Tags

   C-terminal BPNLS

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSynapsin1-FLEx-axon-GCaMP6s (AAV5)

    Viral Prep

    #112010-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-hSynapsin1-FLEx-axon-GCaMP6s (#112010). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-FLEx-axon-GCaMP6s plasmid DNA. Synapsin-driven, cre-dependent axon targeted GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Lin Tian

    Article

    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

    Promoter

    Syn

    Tags

    None

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only