We narrowed to 8,732 results for: Set

1. psiTE(2)-p65AD

   Plasmid

   #220457

   Purpose

   Split intein version of Trojan-p65AD. Create p65AD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.

   Depositor

   Benjamin White

   Article

   Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

   Insert

   CfaN-p65AD-CfaC
   

   Expression

   Insect

   Available Since

   June 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSGKP_AcsgRNA

   Plasmid

   #203807

   Purpose

   pSGKP-based plasmid containing a J23119-driven sgRNA cassette, with replaceable spacer (BsaI-restriction sites). Kanamycin resistance.

   Depositor

   Quanjiang Ji

   Article

   Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

   Insert

   AcgRNA
   

   Use

   CRISPR

   Promoter

   J23119

   Available Since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. FRP1639_PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

   Plasmid

   #58439

   Purpose

   Cassette to replace yeast promoters with an inducible promoter containing 1 lexA box

   Depositor

   Joerg Stelling

   Article

   Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

   Insert

   PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1
   

   Expression

   Yeast

   Promoter

   PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

   Available Since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGolden-Hyg

   Plasmid

   #51423

   Purpose

   Contain one loxP-PGK-Hyg-pA-loxP cassette, used for golden-gate reaction

   Depositor

   Yonglun Luo

   Article

   Luo et al Biotechniques. 2014 May 1;56(5):263-8. d

   Insert

   PGK-Hyg-pA (PGK1 Human)
   

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAH237

   Plasmid

   #121146

   Purpose

   Expression of both nmt41p-cas9 and rrk1p-gRNA (LEU2 marker) for CRISPR genome editing in fission yeast

   Depositor

   Katsunori Tanaka

   Article

   Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

   Inserts

   gRNA cassette
   humanized Streptococcus pyogenes Cas9
   

   Use

   CRISPR

   Tags

   Flag

   Expression

   Yeast

   Promoter

   S.pombe rrk1 and nmt41

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCORE-Kp53

   Plasmid

   #72241

   Purpose

   Contains the CORE cassette with KanMX4 and human p53 gene markers

   Depositor

   Michael Resnick, Francesca Storici

   Article

   Storici et al Genet Eng (N Y). 2003;25:189-207.

   Inserts

   p53 (TP53 Human)
   kanMX4
   

   Expression

   Bacterial

   Mutation

   V122A mutation

   Available Since

   Jan. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pADH119

   Plasmid

   #90985

   Purpose

   NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb LEUpOUT CRISPR system.

   Depositor

   Aaron Hernday

   Article

   Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

   Insert

   gRNA conserved, C. albicans LEU2 2 of 2
   

   Use

   CRISPR

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. IQ013: pMVP/Ad-DEST

   Plasmid

   #121843

   Purpose

   pMVP destination vector, empty adenovirus vector backbone

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Adenoviral; Pmvp gateway destination vector

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHSP70B-CP6-bsr

   Plasmid

   #165878

   Purpose

   Basic Blasticidin resistance cassette in Alpha1, lacks physical marker or attB site

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Basic Selection Vector
   

   Use

   Synthetic Biology

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMA-SpCas9-g10

    Plasmid

    #80793

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 10/20/30 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMA-SpCas9-g9

    Plasmid

    #80792

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 9/19/29 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMA-SpCas9-g8

    Plasmid

    #80791

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 8/18/28 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMTIKA

    Plasmid

    #22061

    Depositor

    Maria Castro

    Article

    Xiong et al J Virol. 2006 Jan . 80(1):27-37.

    Insert

    mCMV-TetOn expression cassette
    

    Expression

    Mammalian

    Available Since

    Nov. 25, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pMVV098

    Plasmid

    #215304

    Purpose

    Expresses sfGFP under induction with the DAPG Marionette Cassette (PhIFAM + PPhIF-sfGFP) with a BBR1 backbone.

    Depositor

    Brian Pfleger

    Article

    Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Promoter

    PPhIF

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAEF5

    Plasmid

    #136305

    Purpose

    Plasmid encoding the Cas9 gene and a gRNA expression cassette allowing to clone a single or multiple gRNAs for DSBs induction in yeast. Constructed by Aubin Fleiss

    Depositor

    Gilles Fischer

    Article

    Agier et al Methods Mol Biol. 2021;2196:181-198. doi

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Feb. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTAJAK-71 (pESC-NatMXsyn-USER)

    Plasmid

    #78232

    Purpose

    Empty vector, for the insertion of gRNA expression cassettes into the vector.

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR2B-dCas9-KRAB

    Plasmid

    #115547

    Purpose

    Entry cassette for dCas9-KRAB

    Depositor

    Rene Maehr

    Article

    Genga et al Cell Rep. 2019 Apr 16;27(3):708-718.e10.

    Insert

    dCas9-KRAB
    

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Available Since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBS AluYa5rescueA70D

    Plasmid

    #78657

    Purpose

    Generates Alu inserts suitable for rescue

    Depositor

    Astrid Roy-Engel

    Article

    Wagstaff et al PLoS Genet. 2012;8(8):e1002842. doi: 10.

    Insert

    Ya5rescue cassette
    

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. MTK8b_004

    Plasmid

    #123892

    Purpose

    Encodes the 3rd gen. 5' HIV LTR cassette as a type 8b part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    5' HIV LTR (3rd gen.)
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAC1750

    Plasmid

    #127984

    Purpose

    AMA1 plasmid with Aspergillus optimized Cpf1, ble selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    Cpf1
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    codon optimized for Aspergillus nidulans

    Promoter

    Aspergillus nidulans tef1 promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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