We narrowed to 9,221 results for: Set
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Plasmid#112313PurposeCRISPR donor plasmid to tag human transcription factor JUNB with GFPDepositorInsertJUNB homology arms flanking EGFP-IRES-Neo cassette (JUNB Human)
UseCRISPRMutationhg38:19:12792992:C>G, hg38:19:12793018:T>G,…Available SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFLIP43
Plasmid#65618PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-edAFPt9-attR1 and attR2-edCer-cMycExpressionBacterialPromoterT7 promoterAvailable SinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
5VC8 (WHSC1)
Plasmid#101262PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceOct. 2, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
vector-dendra2 PCDNA
Plasmid#121134Purposemammalian cell expressionDepositorInsertempty cassete for contol of miR expression
ExpressionBacterialAvailable SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB8622
Plasmid#126912PurposePlasmid containing gRNA expression cassette targeting ADE2 in Saccharomyces cerevisiaeDepositorInsertADE2 gRNA
UseCRISPRAvailable SinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
KaiC CI cat-/EE (E77Q; E78Q; S431E; T432E)
Plasmid#41888DepositorInsertKai C CI cat-/EE
ExpressionBacterialMutationchanged amin acids: 77,78,431 and 432 to glutamatePromotert7Available SinceMarch 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pICSL70005
Plasmid#50335DepositorInsertbialaphos resistance cassette (Nos promoter (A. tumefaciens) + phosphinothricin acetyl transferase (S. hygroscopicus) + Nos term
UsePlant expressionMutationBsaI/BbsI sites removed by point-mutationAvailable SinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
GGE209
Plasmid#120793PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assemblyDepositorInsertInsUp-Mfe
UseCloning/assembly plasmidAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFLIP37
Plasmid#65505PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-Cit-attR1 and attR2-Cer-cMycExpressionBacterialPromoterT7 promoterAvailable SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
YN2_1_LT84_RPL13A
Plasmid#177712PurposeExpresses Cas9 and sgRNA cassette for targeting RPL13A in yeast Saccharomyces cerevisiaeDepositorInsertCas9
UseCRISPRExpressionYeastAvailable SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCH001
Plasmid#47953PurposetetO-GFP reporter casetteDepositorInsertGFP
UseTet inducible expressionExpressionYeastPromoterT3Available SinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
Thy1.2-Roxed-Cre
Plasmid#51276PurposeExpression of the Dre-dependent Roxed-Cre gene under the control of the Thy1.2 expression cassetteDepositorInsertRoxed-Cre
PromoterThy1.2Available SinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAW8ENdeI-cHAIAA7-spo5DSR
Plasmid#49873Purposefor Cre-lox cassette exchange of C-terminal HA-auxin-inducible degron tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locusDepositorTypeEmpty backboneUseCre/LoxTagsIAA17 degron tagAvailable SinceJan. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA
Plasmid#162802PurposeSTELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient expressionDepositorInsertUb-BsmBI-HA
UsePiggybacTagsHAExpressionMammalianPromoterEF1alphaAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDLX1-donor
Plasmid#112343PurposeCRISPR donor plasmid to tag human transcription factor DLX1 with GFPDepositorInsertDLX1 homology arms flanking EGFP-IRES-Neo cassette (DLX1 Human)
UseCRISPRMutationhg38:2:172087403:G>C,hg38:2:172087433:G>A,h…Available SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
GGE094
Plasmid#120787PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by SfiI restriction enzyme after the assemblyDepositorInsertInsDown_Zeta-SfiI
UseCloning/assembly plasmidAvailable SinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
GGE091
Plasmid#120786PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by SfiI restriction enzyme after the assemblyDepositorInsertInsUp_Zeta-SfiI
UseCloning/assembly plasmidAvailable SinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT374_pattB-pCA-GFP-pA-attBSwa
Plasmid#52553Purposefor PhiC31 integrase-mediated cassette exchangeDepositorInsertsPhiC31o attB (full length)
GFP
ExpressionMammalianAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMM0623
Plasmid#128998PurposepRPL18B-Zif268DBD-CRY2PHR cassette plasmid flanked by ConL1 and ConR2DepositorInsertConL1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-ConR2 AmpR-ColE1
ExpressionYeastAvailable SinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0624
Plasmid#128999PurposepZF(3BS)-mRUBY2 cassette plasmid flanked by ConL2 and ConREDepositorInsertConL2-pZF(BS)-mRUBY2-tADH1-ConRE AmpR-ColE1
ExpressionYeastAvailable SinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only