We narrowed to 9,378 results for: mel
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Plasmid#31216DepositorAvailable SinceApril 26, 2012AvailabilityAcademic Institutions and Nonprofits only
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pCDF1-GRM3 WT
Plasmid#31798DepositorAvailable SinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCALNL-Rheb(S16H)
Plasmid#194204PurposeCre/LoxP-dependent expression of a constitutively active form of Rheb (S16H). Expression of Cre excises a STOP codon before Rheb(S16H), facilitating expression of a constitutively active form of Rheb.DepositorInsertRas Homolog Enriched Protein in Brain (S16H) (RHEB Human)
UseCre/LoxExpressionMammalianMutationS16HAvailable SinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
mouse Oct4-GFP GOF18 transgenic reporter
Plasmid#60527PurposeBAC recombineering generated construct, used as a reporter for Oct4 expression (containes both DE and PE elements)DepositorInsertGFPpA
ExpressionMammalianPromoterOct4Available SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF752
Plasmid#88486PurposeDonor Vector containing ZNF752 transcription factor, part of the Human TFome CollectionDepositorInsertZNF752 (ZFP3 Human)
UseGateway CloningMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330 Ctnnb1.1
Plasmid#59911PurposepX330 backbone expressing sgRNA targeting Ctnnb1 to edit mouse beta-Catenin. Expresses Cas9 from CBh promoterDepositorAvailable SinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHIV-BRAFV600E-mOrange2
Plasmid#110732PurposeMammalian Expression of BRAFV600E IRES H2B-mOrange2DepositorInsertBRAFV600E IRES H2B-mOrange2 (BRAF Human)
UseLentiviralExpressionMammalianMutationBRAF V600EAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF785
Plasmid#88459PurposeDonor Vector containing ZNF785 transcription factor, part of the Human TFome CollectionDepositorInsertZNF785 (ZNF785 Human)
UseGateway CloningMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF688_isoform1
Plasmid#88779PurposeDonor Vector containing ZNF688 transcription factor, part of the Human TFome CollectionDepositorInsertZNF688 (ZNF688 Human)
UseGateway CloningMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-GFP
Plasmid#122100PurposeAAV-mediated expression of GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.DepositorHas ServiceAAV2InsertGFP
UseAAVTagsNAExpressionMammalianPromoterEF1a(1.1kb short version)Available SinceMay 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
SopB(C460S)-EGFP
Plasmid#183656PurposeC-terminally tagged Salmonella Typhimurium SopB (catalytically inactive) for mammalian expressionDepositorInsertsopB (sopB Salmonella enterica serovar Typhimurium)
TagsEGFPExpressionMammalianMutationC460SPromoterCMVAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-mLin28A
Plasmid#26357DepositorAvailable SinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLX317-CELF4
Plasmid#115445PurposeConstitutive lentiviral expressionDepositorAvailable SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
K9L mutant of H3K9me3 biosensor
Plasmid#120807PurposeFRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capabilityDepositorInsertTruncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
ExpressionMammalianMutationlysine 9 is mutated to leucine 9 on histone H3PromoterCMVAvailable SinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pL40C_PGKintron_Cas9_Green
Plasmid#134966PurposeLentiviral vector coding Cas9 and mNeonGreenDepositorInsertCas9-P2A-mNeonGreen
UseCRISPR and LentiviralTagsFLAGExpressionMammalianPromoterhPGK promoter with beta-globin intronAvailable SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG CLTX-CAR
Plasmid#157743Purposeknock CLTX-CAR into AAVS1 safe harbor locus for constitutive expressionDepositorInsertchlorotoxin containing chimeric antigen receptor targeting glioblastoma
UseCRISPR, Synthetic Biology, and TALENExpressionMammalianPromoterCAGAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
W45A mutant of H3K9me3 biosensor
Plasmid#120808PurposeFRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capabilityDepositorInsertTruncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
ExpressionMammalianMutationTryptophan 45 is mutated to Alanine 45 on HP1 dom…PromoterCMVAvailable SinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBABE-hygro-2xMyc-Rac1-WT
Plasmid#128580PurposeFor mammalian cell expression of WT murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.DepositorAvailable SinceAug. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Circular 200,100 (mIDUA)
Plasmid#170123PurposeAAV vector carrying 2 copies of a guide RNA targeting the mouse IDUA mRNA, expressed from human and mouse U6 promotersDepositorInsertCircular 200,100 guide RNA (Idua Mouse)
UseAAVExpressionMammalianPromoterHuman U6 and mouse U6Available SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti_CRISPRi_sgAR2
Plasmid#167001PurposeLentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdownDepositorAvailable SinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only