We narrowed to 18,318 results for: Mut

1. pNLF1-C_hCOL4A5-Nluc_R1683X

   Plasmid

   #198124

   Purpose

   Express hCOL4A5_R1683X in mammalian cells

   Depositor

   Jeffrey Miner

   Article

   Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

   Insert

   COL4A5 (COL4A5 Human)
   

   Use

   Tags

   Nluc

   Expression

   Mammalian

   Mutation

   R1683X

   Promoter

   CMV, beta-globin/IgG chimeric intron

   Available Since

   April 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pF149 pSOD1G85RAcGFP1

   Plasmid

   #26410

   Depositor

   Elizabeth Fisher

   Article

   Stevens et al PLoS One. 2010 . 5(3):e9541.

   Insert

   SOD1 (SOD1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   G85R

   Promoter

   Available Since

   Nov. 8, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pENTR221-FGFR4

   Plasmid

   #60531

   Purpose

   Entry vector for human FGFR4

   Depositor

   John Brognard

   Article

   Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

   Insert

   FGFR4 (FGFR4 Human)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   c2134a P712T

   Promoter

   NA

   Available Since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pF147 pSOD1A4VAcGFP1

   Plasmid

   #26408

   Depositor

   Elizabeth Fisher

   Article

   Stevens et al PLoS One. 2010 . 5(3):e9541.

   Insert

   SOD1 (SOD1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   A4V

   Promoter

   Available Since

   Nov. 8, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pB-CAG-B19L-IRES-mCherry-WPRE-BGHpA

   Plasmid

   #178518

   Purpose

   For making Rabies virus SAD B19 strain L cell line with hyPBase

   Depositor

   Ian Wickersham

   Article

   Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

   Insert

   B19L-IRES-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. TDP-43_CTD_del321-343

   Plasmid

   #98676

   Purpose

   Expresses 6xHis-tagged C-terminal domain of TDP-43 with a deletion of AA 321-343

   Depositor

   Nicolas Fawzi

   Article

   Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   del321-343

   Promoter

   Available Since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQCXIP GFP-MOSPD2 RD/LD

   Plasmid

   #186468

   Purpose

   Expression of human MOSPD2 (mutant R404D/L406D, unable to bind FFAT motifs) fused to EGFP in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

   Insert

   MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   R404D/L406D (mutant unable to bind FFAT motifs)

   Promoter

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK5-myc-Miro1 K427V

   Plasmid

   #47892

   Purpose

   Expresses myc tagged Miro1 K427 constitutively active mutant

   Depositor

   Pontus Aspenström

   Article

   Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

   Insert

   Miro1 K427V (RHOT1 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   K427V, constitutively active mutant

   Promoter

   CMV

   Available Since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pαH-S-RRAR

   Plasmid

   #164569

   Purpose

   SARS-CoV-2 Spike protein with Furin site restored (S-RRAR variant)

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

   Insert

   Spike (S-RRAR variant)
   

   Use

   Tags

   2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCEP4-myc-CCR5

    Plasmid

    #98948

    Purpose

    Mammalian expression plasmid for N-terminal myc-tagged human CCR5

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CCR5 (CCR5 Human)
    

    Use

    Tags

    Signal/leader sequence from Influenza A virus HA …

    Expression

    Mammalian

    Mutation

    Full-length, wildtype

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. myc-KIF5A

    Plasmid

    #127616

    Purpose

    N-terminal myc-tagged mouse KIF5A

    Depositor

    Josef Kittler

    Article

    Twelvetrees et al Neuron. 2010 Jan 14;65(1):53-65. doi: 10

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDG335

    Plasmid

    #100899

    Purpose

    SpCas9n (D10A Nickase mutant) with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D10A mutant converts to Nickase

    Promoter

    CBh and U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. VPS13C^halo∆1235-1748

    Plasmid

    #187297

    Purpose

    Express VPS13C truncation mutant in mammalian cells

    Depositor

    Pietro De Camilli

    Article

    Cai et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

    Insert

    VPS13C truncation mutant (VPS13C Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-CMV-cGAS C396/7A-HA

    Plasmid

    #130917

    Purpose

    Expresses human cGAS-HA with C396/397A point mutations; Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    cGAS C396/7A
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    mutated residues 396 and 397 to alanines

    Promoter

    CMV

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLentiPGK Puro DEST JNKKTR AA Clover

    Plasmid

    #90238

    Purpose

    Lentiviral vector to express JNK KTR AA (mutant) mClover under PGK promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

    Insert

    JNK Kinase Translocation Reporter (AA mutant)
    

    Use

    Lentiviral

    Tags

    mClover

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPGS dnTcf-4(deltaN41)

    Plasmid

    #19284

    Depositor

    Eric Fearon

    Article

    Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

    Insert

    Tcf-4 (TCF4 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mutation

    Dominant negative, lacks N-terminal 41 amino acids

    Promoter

    Available Since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAc-y1sgRNA-Cas9

    Plasmid

    #49331

    Purpose

    Expresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cells

    Depositor

    Ji-Long Liu

    Article

    Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

    Inserts

    Cas9
    dU6-sgRNA
    y1 sgRNA (y Fly)
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Insect

    Mutation

    Human codon optimised

    Promoter

    Actin-5c and Drosophila U6

    Available Since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-EF1α-ZC3H12D-D95N-IRES-EGFP

    Plasmid

    #153071

    Purpose

    Lentiviral vector expressing human ZC3H12D mutated (D95N)

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    Mutated ZC3H12D (D95N) (ZC3H12D Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed D95 to N, known to abrogate the RNase enz…

    Promoter

    EF1α

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-Tag2-Sox9 T236A

    Plasmid

    #111455

    Purpose

    Transient expression of Flag-tagged human Sox9 with T236A mutation

    Depositor

    Sharon Pine

    Article

    Hong et al Nucleic Acids Res. 2016 Oct 14;44(18):88

    Insert

    Sox9 (SOX9 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T236A mutation

    Promoter

    CMV

    Available Since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pETM-60-NusA-TEV-HsRCK_296-472_E

    Plasmid

    #146209

    Purpose

    Bacterial Expression of HsRck_296-472. Contains a TEV site rather than a HRV 3C site downstream of NusA.

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

    Insert

    HsRck_296-472 (DDX6 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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