We narrowed to 15,824 results for: LEA

1. MO70 - Orai1 EC-Flag

   Plasmid

   #79590

   Purpose

   Transient expression and retroviral Expression

   Depositor

   Yousang Gwack

   Article

   Srikanth et al J Biol Chem. 2010 Feb 12;285(7):5066-75.

   Insert

   Orai1 (ORAI1 Human)
   

   Use

   Retroviral

   Tags

   FLAG (inserted between TM3 and TM4 of the protein…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pCAG-lox-inv[Talpha1-iCre-pA]-lox-Lyn-EGFP

   Plasmid

   #196875

   Purpose

   Neuron-specific expression of LynGFP reporter

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   LynEGFP
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Quimeric CAG

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSOMA-T679A-NES

   Plasmid

   #118940

   Purpose

   Used to express the T679A mutant form of the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thaliana

   Depositor

   Patrick Krysan

   Article

   Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

   Insert

   SOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Exclusion Signal
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pClneoMyc MOB1 T74A

   Plasmid

   #47939

   Purpose

   expresses Myc tagged human MOB1 containing T74A mutation

   Depositor

   Yutaka Hata

   Article

   Bao et al Genes Cells. 2009 Dec;14(12):1369-81. do

   Insert

   MOB1A (MOB1A Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   T74A

   Promoter

   CMV

   Available since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pGBT9-Hook2

   Plasmid

   #198524

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-Hook2 fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

   Insert

   Hook2 (HOOK2 Human)
   

   Use

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Mutation

   His488Gln substitution

   Promoter

   ADH1

   Available since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28b-API5

   Plasmid

   #157655

   Purpose

   Expresses API5 in E.coli cell

   Depositor

   Byung Il Lee

   Article

   Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

   Insert

   Apoptosis inhibitor 5 (API5 Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. sTR056

   Plasmid

   #177369

   Purpose

   Toehold switch, with an unpaired toehold region at the 5′-end that interacts with the trigger RNA (sTR060, Addgene plasmid # 177370) to unfold the switch.

   Depositor

   Jerome Bonnet, Jean-Loup Faulon

   Article

   Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

   Insert

   sfGFP
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. sTR060

   Plasmid

   #177370

   Purpose

   Trigger RNA (GCAGGGATAAACGAGATAGATAAGATAAGA) that pairs with the toehold region in sTR056 (Addgene plasmid #177369) to restore translational activity.

   Depositor

   Jerome Bonnet, Jean-Loup Faulon

   Article

   Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

   Insert

   Trigger RNA
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2+ oDam_f_GFPoNLS

   Plasmid

   #85819

   Purpose

   iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

   Depositor

   Joachim Wittbrodt

   Article

   Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

   Insert

   oDam_f_GFPoNLS
   

   Use

   Tags

   oNLS (optimized nuclear localization signal) C te…

   Expression

   Bacterial, Insect, Mammalia…

   Mutation

   L122A. Codon optimization compatible to work in M…

   Promoter

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. p3xFLAG-MKL1 D301-380

    Plasmid

    #19852

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 (MRTFA Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    deleted amino acids 301 to 380

    Promoter

    Available since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pEGFP-YOD1-MutS2

    Plasmid

    #85667

    Purpose

    Expression of human YOD1 I292Q V295Q mutant with N-terminal GFP tag

    Depositor

    Hemmo Meyer

    Article

    Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

    Insert

    YOD1 (YOD1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    I292Q and V295Q

    Promoter

    CMV

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-EF1a-V5-Emerin

    Plasmid

    #120246

    Purpose

    Expresses V5-tagged Emerin in lentiviral vector

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Insert

    Emerin (Emd Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. lenti-sgRNA(MS2)_zeo_hPDX1_promoter_guide1

    Plasmid

    #176838

    Purpose

    To induce human PDX1 expression by recruiting SAM complex to PDX1 promoter

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

    Insert

    sgPDX1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSN69

    Plasmid

    #100125

    Purpose

    expresses putative cMYC DBD with a HA tag

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCerulean-P2A-putative cMYC DBD with a HA tag (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Insert corresponds to the DBD of cMYC

    Promoter

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-HA-DNM2-W525L

    Plasmid

    #155263

    Purpose

    Mammalian expression of rat DNM2 fused to HA

    Depositor

    David Rubinsztein

    Article

    Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

    Insert

    dynamin 2 (Dnm2 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Tryptophan 525 to Leucine

    Promoter

    Available since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBabe-ER-E2F2-dRb

    Plasmid

    #62805

    Purpose

    expresses an inducible E2F2 mutant that lacks the Rb binding domain

    Depositor

    Gerardo Ferbeyre

    Article

    Vernier et al Genes Dev. 2011 Jan 1;25(1):41-50. doi:

    Insert

    E2F2 (E2F2 Human)
    

    Use

    Retroviral

    Tags

    HA-Er

    Expression

    Mammalian

    Mutation

    deletion of amino acids 302-437, including the Rb…

    Promoter

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. HOXB1 (human) HIS-tag pET

    Plasmid

    #8520

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXB1 (HOXB1 Human)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    PCR from cDNA clone

    Promoter

    Available since

    July 6, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pClneoMyc MOB1 T12A

    Plasmid

    #47936

    Purpose

    expresses Myc tagged human MOB1 containing T12A mutation

    Depositor

    Yutaka Hata

    Article

    Bao et al Genes Cells. 2009 Dec;14(12):1369-81. do

    Insert

    MOB1A (MOB1A Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    T12A

    Promoter

    CMV

    Available since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA4-hB90

    Plasmid

    #18968

    Depositor

    Mariano Viapiano

    Article

    Hu et al J Biol Chem. 2008 Sep 5;283(36):24848-59

    Insert

    Brevican C-terminus (BCAN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Human brevican signal peptide aa 1-23 followed by…

    Promoter

    Available since

    Dec. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pX330-NQL007-NANOG-sgRNA

    Plasmid

    #175555

    Purpose

    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse NANOG locus.

    Depositor

    Elzo de Wit

    Article

    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

    Insert

    spCas9-nuclease and sgRNA against mouse NANOG STOP Codon
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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