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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,067 results for: LEA

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Showing: 6541 - 6560 of 14067 results

  3. pCAG-LbCas12a-Plus-mCherry

    Plasmid
    #191230
    Purpose
    To express the LbCas12a nuclease variant "LbCas12a-Plus" that enhanced the editing activity and specificity
    Depositor
    Zhili Rong
    Publication
    Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LbCas12a-Plus-P2A-mCherry
    Use
    CRISPR
    Mutation
    D156R, R883K, R887A
    Available since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    191230-plasmid-map-sequence-id-404705

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  4. pMAH-ER-bapaUGAc

    Plasmid
    #201674
    Purpose
    boronic acid- and peptide-assisted UDP-GlcNAc sensor in ER
    Depositor
    Huiwang Ai
    Publication
    Zhang et al ACS Sens. 2023 Jul 22. doi: 10.1021/acss
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ER-bapaUGAc
    Tags
    ER leading sequence and ER retention sequence
    Expression
    Mammalian
    Promoter
    CMV SP6
    Available since
    Aug. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    201674-plasmid-map-sequence-id-398164

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  5. pCAGGS-N-TEV-HAT

    Plasmid
    #202598
    Purpose
    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell
    Depositor
    Chiaki Murakami
    Publication
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Type
    Empty backbone
    Tags
    TEV cleavable site (ENLYFQG) and poly-histidine a…
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202598-plasmid-map-sequence-id-399468

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  8. FnR9-10

    Plasmid
    #182567
    Purpose
    Human fibronectin fragment, type-III repeats 9-10, R1379A mutant
    Depositor
    Timothy Springer
    Publication
    Su et al J Biol Chem. 2022 Sep;298(9):102323. doi
    Insert
    Human fibronectin fragment, type-III repeats 9-10
    Tags
    His and TEV cleavage site after His tag
    Expression
    Bacterial
    Mutation
    R1379A
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182567-plasmid-map-sequence-id-357181

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  12. pCDF-NtXR1AtR2B2NtS1

    Plasmid
    #160879
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana Rubisco chaperone proteins raf2, and bsd2 in E. coli.
    Depositor
    Maureen Hanson
    Publication
    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do
    Insert
    rbcS-S1, rbcX, raf1, raf2, bsd2
    Expression
    Bacterial
    Promoter
    rbcS-S1: pBAD, chaperones: T7
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160879-plasmid-map-sequence-id-318813

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  15. 5THA (Gemin5)

    Plasmid
    #101890
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Gemin5 (GEMIN5 Human)
    Use
    Baculovirus expression
    Tags
    6xHis, Thrombin cleavage
    Promoter
    polyhedrin
    Available since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    101890-plasmid-map-sequence-id-194723

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  16. 5WBN (HDAC6)

    Plasmid
    #101267
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HDAC6 (HDAC6 Human)
    Tags
    Hexahistidine tag with Thrombin cleavage
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 2, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101267-plasmid-map-sequence-id-193501

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  20. pEG03

    Plasmid
    #73841
    Purpose
    Encodes PhyB917 with a C-terminal histidine tag, for protein expression in Dicty.
    Depositor
    Junghae Suh, Jeffrey Tabor
    Publication
    Gomez et al ACS Nano. 2015 Nov 30.
    Insert
    PhyB(1-917) with C-term his tag (PIL2 Mustard Weed)
    Use
    Dictyostelium discoideum expression
    Tags
    6x his tag
    Available since
    June 28, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 6541 - 6560 of 14067 results