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pdCas12f1Cas_ABE-LVA

Plasmid

#220995

Purpose

Prokaryotic and constitutive expression of AsCas12f1/TadA8e-LVA fusion

Depositor

John van der Oost

Article

Swartjes et al iScience. 2025 Nov 12;28(12):114033. doi

Insert

Acidibacillus sulfuroxidans Cas12f1-ABE

Use

CRISPR

Tags

LVA

Expression

Bacterial

Mutation

D225A

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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LentiGuide Cyclone-Puro P2A EGFP

Plasmid

#247497

Purpose

Acyclovir-regulated puromycin n-acetyltransferase-P2A-EGFP fusion protein

Depositor

Samie Jaffrey

Article

Hou et al Nat Methods. 2025 Nov 3. doi: 10.1038/s4

Insert

puromycin n-acetyltransferase with Cyclone insertion

Use

Lentiviral and Synthetic Biology

Tags

Puro(Cyclone)-P2A-EGFP

Expression

Mammalian

Mutation

Cyclone was inserted after Glutamine135 of puromy…

Promoter

EF-1a

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_DualsgRNA_TIMM23_ER-dAPEX-BFP2

Plasmid

#236734

Purpose

Dual sgRNA targeting TIMM23 expressing dAPEX-BFP targeting to ER

Depositor

Derek Narendra

Article

Thayer et al EMBO J. 2025 Nov 20. doi: 10.1038/s44318

Insert

TIMM23 (TIMM23 Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKH032

Plasmid

#224548

Purpose

2A-3xNLS-2xClover mouse Car2 C-term targeting vector

Depositor

Elphege Nora

Article

Hansen et al Science. 2025 Nov 27:eadt4221. doi: 10.1

Insert

2xClover

Use

Mouse Targeting

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKH040

Plasmid

#224549

Purpose

2A-3xNLS-tdTomato mouse Car2 C-term targeting vector

Depositor

Elphege Nora

Article

Hansen et al Science. 2025 Nov 27:eadt4221. doi: 10.1

Insert

tdTomato

Use

CRISPR and Mouse Targeting

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-Syb2-R56X

Plasmid

#248137

Purpose

Mammalian expression lentiviral vector encoding the wild-type Synaptobrevin2 (Syb2) with R56X mutation

Depositor

Ege Kavalali

Article

Guzikowski et al Proc Natl Acad Sci U S A. 2025 Nov 4;122

Insert

Syb2-R56X (Vamp2 Rat)

Expression

Mammalian

Mutation

R56X

Promoter

Ubiquitin

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-Syb2-V43del

Plasmid

#248139

Purpose

Mammalian expression lentiviral vector encoding the wild-type Synaptobrevin2 (Syb2) with V43del deletion

Depositor

Ege Kavalali

Article

Guzikowski et al Proc Natl Acad Sci U S A. 2025 Nov 4;122

Insert

Syb2-V43del (Vamp2 Rat)

Expression

Mammalian

Mutation

V43del

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-Syb2-S75P

Plasmid

#248138

Purpose

Mammalian expression lentiviral vector encoding the wild-type Synaptobrevin2 (Syb2) with S75P mutation

Depositor

Ege Kavalali

Article

Guzikowski et al Proc Natl Acad Sci U S A. 2025 Nov 4;122

Insert

Syb2-S75P (Vamp2 Rat)

Expression

Mammalian

Mutation

S75P

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-Syb2-R56L

Plasmid

#248136

Purpose

Mammalian expression lentiviral vector encoding the wild-type Synaptobrevin2 (Syb2) with R56L mutation

Depositor

Ege Kavalali

Article

Guzikowski et al Proc Natl Acad Sci U S A. 2025 Nov 4;122

Insert

Syb2-R56L (Vamp2 Rat)

Expression

Mammalian

Mutation

R56L

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-Syb2-G73W

Plasmid

#248135

Purpose

Mammalian expression lentiviral vector encoding the wild-type Synaptobrevin2 (Syb2) with G73W mutation

Depositor

Ege Kavalali

Article

Guzikowski et al Proc Natl Acad Sci U S A. 2025 Nov 4;122

Insert

Syb2-G73W (Vamp2 Rat)

Expression

Mammalian

Mutation

G73W

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-Syb2-F77S

Plasmid

#248134

Purpose

Mammalian expression lentiviral vector encoding the wild-type Synaptobrevin2 (Syb2) with F77S mutation

Depositor

Ege Kavalali

Article

Guzikowski et al Proc Natl Acad Sci U S A. 2025 Nov 4;122

Insert

Syb2-F77S (Vamp2 Rat)

Expression

Mammalian

Mutation

F77S

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-Syb2-A67P

Plasmid

#248132

Purpose

Mammalian expression lentiviral vector encoding the wild-type Synaptobrevin2 (Syb2) with A67P mutation

Depositor

Ege Kavalali

Article

Guzikowski et al Proc Natl Acad Sci U S A. 2025 Nov 4;122

Insert

Syb2-A67P (Vamp2 Rat)

Expression

Mammalian

Mutation

A67P

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-Syb2-I45del

Plasmid

#248140

Purpose

Mammalian expression lentiviral vector encoding the wild-type Synaptobrevin2 (Syb2) with I45del deletion

Depositor

Ege Kavalali

Article

Guzikowski et al Proc Natl Acad Sci U S A. 2025 Nov 4;122

Insert

Syb2-I45del (Vamp2 Rat)

Expression

Mammalian

Mutation

I45del

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-Syb2-E78A

Plasmid

#248133

Purpose

Mammalian expression lentiviral vector encoding the wild-type Synaptobrevin2 (Syb2) with E78A mutation

Depositor

Ege Kavalali

Article

Guzikowski et al Proc Natl Acad Sci U S A. 2025 Nov 4;122

Insert

Syb2-E78A (Vamp2 Rat)

Expression

Mammalian

Mutation

E78A

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-SNAPf-Sec61b

Plasmid

#247295

Purpose

Mammalian expression of the SNAPf-tagged ER reporter SEC61b; to label ER membranes.

Depositor

Jennifer Lippincott-Schwartz

Article

Choi et al Nature. 2025 Nov 5. doi: 10.1038/s41586-

Insert

SNAPf-Sec61b (SEC61B Human)

Tags

SnapTag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pBK1838

Plasmid

#239792

Purpose

pAAV-CMV-SNCAintron1-AlphaSyn[A53T]-WPRE-SV40pA (PD-INDUCER)

Depositor

Boris Kantor

Article

Kantor et al Mol Ther. 2018 Nov 7;26(11):2638-2649. d

Insert

CMV/SNCAintron1-AlphaSyn[A53T]-WPRE-SV40pA

Use

AAV

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pBK1575

Plasmid

#239793

Purpose

pLV-U6-[empty]-EFSNC-dSaCas9-[empty linker]-P2A-Puro-WPRE

Depositor

Boris Kantor

Article

Kantor et al Mol Ther. 2018 Nov 7;26(11):2638-2649. d

Insert

pLV-U6-[empty]-EFSNC-dSaCas9-[empty linker]-P2A-Puro-WPRE

Use

Lentiviral

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pU6_H1-g3

Plasmid

#247169

Purpose

U6 driven gRNA expression of H1-g3 for targeting Dn29 attH1

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

H1-g3

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pFB-CMV-DIO-MTS-roGFP-WPRE

Plasmid

#245375

Purpose

Cre-dependent expression of a green fluorescent protein biosensor to determine mitochondrial cellular reduction-oxidation (redox) states.

Depositor

D. James Surmeier

Article

Tubert et al Neurobiol Dis. 2023 Nov;188:106328. doi:

Insert

roGFP

Use

AAV

Tags

mitochondrial-targeting sequence of the S. pombe …

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pSB02

Plasmid

#230991

Purpose

B. dendrobatidis plasmid for integration of target sequence at URA3 locus, blasticidin S selection

Depositor

Lillian Fritz-Laylin

Article

Brody et al Proc Natl Acad Sci U S A. 2025 Nov 4;122

Insert

BdURA3_LHA2-Hsbsr-ScADH1ter

Use

Bd gene targeting vector

Tags

bsr

Expression

Bacterial

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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