191,377 results

1. pB_tetO7_sfGFP

   Plasmid

   #196616

   Purpose

   Plasmid for integrating tetO7-controlled sfGFP into the HO region in yeast

   Depositor

   Matthias Heinemann

   Article

   Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

   Insert

   Superfolder GFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   tetO7 promoter

   Available since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV.CAG.(cyto).iATPSnFR2.S29W.A95K.HaloTag

   Plasmid

   #209651

   Purpose

   Expression of iATPSnFR2, high affinity

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.S29W.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mutation

   S29W.A95K

   Promoter

   CAG

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. NC16 pCDNA3.1 FLAG NRF2

   Plasmid

   #36971

   Depositor

   Randall Moon

   Article

   Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

   Insert

   NRF2 (NFE2L2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEX-A-U6-gRNA

   Plasmid

   #65626

   Purpose

   Empty vector for the expression U6 driven gRNA

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   U6-gRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTXB1.Tat(48-57)-Cre recombinase

   Plasmid

   #165042

   Purpose

   Expression of Tat(48-57) conjugated Cre recombinase for protein purification (Intein-chitin binding domain fussion)

   Depositor

   Dokyun Na

   Article

   Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

   Insert

   Cre recombinase
   

   Use

   Tags

   Mxe Intein-Chitin binding domain and Tat(48-57)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB533_15F11-sfGFP

   Plasmid

   #167526

   Purpose

   H4K20me1-mintbody expression vector for mammalian cells

   Depositor

   Hiroshi Kimura

   Article

   Sato et al J Mol Biol. 2016 Oct 9;428(20):3885-3902

   Insert

   15F11-scFv
   

   Use

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE (AAV5)

   Viral Prep

   #202199-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE (#202199). In addition to the viral particles, you will also receive purified pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE plasmid DNA. Synapsin-driven, Cre-dependent expression of bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized opsin PdCO. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Promoter

   Syn

   Tags

   EGFP

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

8. FLAG-SENP6m

   Plasmid

   #18716

   Depositor

   Edward Yeh

   Article

   Dou et al Mol Cell. 2010 Aug 13. 39(3):333-45.

   Insert

   FLAG-SENP6m (SENP6 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   C1030A

   Promoter

   Available since

   June 20, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pLVX-EF1a-GFP-LaminA-IRES-Hygromycin

   Plasmid

   #134867

   Purpose

   Expresses EGFP-tagged nuclear envelope marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Lamin A (LMNA Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV.CAG.Flex.GCaMP6s.WPRE.SV40

    Plasmid

    #100842

    Purpose

    AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

    Promoter

    CAG

    Available since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-dAPEX2

    Plasmid

    #117173

    Purpose

    Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    dAPEX2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    W41F and A134P on soybean APX

    Promoter

    EF1a

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA-14-3-3 zeta

    Plasmid

    #116888

    Purpose

    Expresses HA-tagged human 14-3-3 zeta in mammalian cells

    Depositor

    FENG-QIAN Li, Ken-Ichi Takemaru

    Article

    Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

    Insert

    14-3-3 zeta (YWHAZ Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDH-TfR-mApple

    Plasmid

    #179384

    Purpose

    Lentiviral vector for expression of Human Transferrin receptor-1 fused mApple

    Depositor

    Angus Johnston

    Article

    Rennick et al Nat Commun. 2022 Oct 12;13(1):6023. doi:

    Insert

    Transferrin receptor 1 - mApple (TFRC Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. W118-1_hTGM2_C277S-Flag

    Plasmid

    #180412

    Purpose

    lentiviral transduction of human TGM2 transcript variant 2 with a point mutation (C277S) that inactivate the enzyme and also with a C-terminal Flag tag

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    TGM2_C277S (TGM2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C277S

    Promoter

    CMV

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. CRY2 mCherry Sec61B

    Plasmid

    #102246

    Purpose

    Expresses fusion of CRY2PHR with mCherry and Sec61B

    Depositor

    Bianxiao Cui

    Article

    Che et al ACS Synth Biol. 2015 Oct 16;4(10):1124-3

    Insert

    CRY2PHR-mCherry-SEC61beta (CRY2 Mustard Weed, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYIC

    Plasmid

    #18673

    Depositor

    Han Htun

    Article

    Nie et al Nucleic Acids Res. 2006 . 34(19):5528-4

    Type

    Empty backbone

    Use

    Tags

    3Myc-EYFP-HA-His6 and ECFP-HA-His6

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmiRFP670-N1

    Plasmid

    #79987

    Purpose

    Monomeric near-infrared fluorescent protein miRFP670 in mammalian plasmid

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Has Service

    Cloning Grade DNA

    Insert

    miRFP670
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV4-BlaM-Vpr

    Plasmid

    #21950

    Depositor

    Warner Greene

    Article

    Cavrois et al Nat Biotechnol. 2002 Nov . 20(11):1151-4

    Insert

    BlaM-Vpr (vpr Fusion of BlaM and HIV-1 Vpr)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

19. PB-TO-NFIA.1-SOX9

    Plasmid

    #182310

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Inserts

    NFIA-T2A-SOX9 (NFIA, SOX9 Human)
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Piggybac

    Tags

    None and T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and TRE3G

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEN396 - pCAGGS-Tir1-V5-2A-PuroR TIGRE donor

    Plasmid

    #92142

    Purpose

    Targeting vector to introduce an osTir1 expressing cassette at the mouse TIGRE acceptor locus using Puromycin selection (2A-fusion). Auxin-inducible degron system.

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    osTir1
    

    Use

    Mouse Targeting

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. p40PX-EGFP

    Plasmid

    #19010

    Depositor

    Michael Yaffe

    Article

    Kanai et al Nat Cell Biol. 2001 Jul . 3(7):675-8.

    Insert

    p40phox (NCF4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa1-148 (PX domains)

    Promoter

    CMV

    Available since

    Aug. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSLHDR03_mCherry_tCD19_unique guide

    Plasmid

    #124062

    Purpose

    Construction of 1st donor for scarless genome editing with unique guide

    Depositor

    Matthew Porteus

    Article

    Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

    Inserts

    mCherry
    tCD19
    

    Use

    Mouse Targeting; Human targeting

    Tags

    Expression

    Mutation

    Promoter

    Human UbC modified

    Available since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. pEF6-myc-TBC1D15

    Plasmid

    #79148

    Purpose

    mammalian expression of TBC1D15

    Depositor

    Aimee Edinger

    Article

    Peralta et al J Biol Chem. 2010 May 28. 285(22):16814-

    Insert

    TBC1D15 (Tbc1d15 Mouse)
    

    Use

    Tags

    His, Myc, and V5

    Expression

    Mammalian

    Mutation

    S616N (please see depositor comment below)

    Promoter

    EF-1alpha

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAT15516_BEAR-GFP-2in1

    Plasmid

    #174082

    Purpose

    BEAR-GFP plasmid with a gRNA that targets its own plasmid

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    EGFP split with an intron between amino acids 95-96
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    disrupted 5' splice site

    Promoter

    CMV

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. SpCas9

    Plasmid

    #188489

    Purpose

    Expresses FLAG tagged SpCas9

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAG_Xph20-eGFP-CCR5TC

    Plasmid

    #135530

    Purpose

    Encodes a specific PSD-95 binder (Xph20) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph20
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. FR_HNF1A

    Plasmid

    #31104

    Depositor

    Gerhart Ryffel

    Article

    Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

    Insert

    HNF1A (HNF1A Human)
    

    Use

    Flp/frt

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLVX-EF1alpha-SARS-CoV-2-nsp1-2xStrep-IRES-Puro

    Plasmid

    #141367

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-nsp1 (ORF1ab SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pVoPo-GFP

    Plasmid

    #187840

    Purpose

    Constitutive GFP-expression vector for Fusobacterium nucleatum

    Depositor

    Jörg Vogel

    Article

    Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PBAD-his6-prkA-pACYC184

    Plasmid

    #41041

    Depositor

    Ichiro Matsumura

    Article

    Parikh et al Protein Eng Des Sel. 2006 Mar;19(3):113-

    Insert

    prkA
    

    Use

    Tags

    6xHis and T7 epitope tag

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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