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Showing: 1281 - 1320 of 1648 results

  10. pET23a-H6-TEV-AncSZ

    Plasmid
    #214235
    Purpose
    Bacterial expression construct of AncSZ, an kinase domain engineered through ancestorial reconstruction of the kinase domains of both SYK and ZAP-70. For in vitro protein tyrosine phosphorylation
    Depositor
    Neel Shah
    Article
    Hobbs et al Protein Sci. 2022 Oct;31(10):e4411. doi:
    Insert
    Syk kinase (SYK Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Sequence gained through ancestral reconstruction …
    Promoter
    T7
    Available since
    Feb. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214235-plasmid-map-sequence-id-422817

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  11. pRH3175

    Plasmid
    #210177
    Purpose
    Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-R33G,G141S-GFP::tADH1
    Depositor
    Randy Hampton
    Article
    Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.
    Insert
    YIp ADE2-LEU2 pTDH3::aro7-R33G,G141S-GFP::tADH1 (ARO7 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    R33G,G141S
    Promoter
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    210177-plasmid-map-sequence-id-415795

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  13. pET24a-LaM4-2xTS

    Plasmid
    #162779
    Purpose
    Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to two tyrosine sulfation (TS) motifs. LaM4-2xTS also contains a T7, HA, BAP and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii
    Insert
    anti-mCherry nanobody fused to two TS sites, T7, HA, BAP and His6 epitope
    Use
    Tags
    BAP, HA, His6, T7, and TS site from proCCK
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    162779-plasmid-map-sequence-id-321884

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  14. pcDNA3_HLA-cmyc-GRM3 C127A TMD Y616M

    Plasmid
    #140454
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and Y616M mutant
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, tyrosine 616 to methioni…
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    140454-plasmid-map-sequence-id-292302

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  15. pHR-BTLA (PD-1 ITIM)-mGFP

    Plasmid
    #180818
    Purpose
    Expression of human BTLA with immunoreceptor tyrosine-based inhibitory motif (ITIM) from PD-1, fused to mEGFP
    Depositor
    Enfu Hui
    Article
    Xu et al Elife. 2021 Nov 4;10. pii: 74276. doi: 1
    Insert
    BTLA (PD-1 ITIM) (BTLA Human)
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mutation
    replaced BTLA-ITIM (IVYASL) with PD-1-ITIM (VDYGE…
    Promoter
    SFFV
    Available since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    180818-plasmid-map-sequence-id-356696

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  18. LIC-Z-Y-L

    Plasmid
    #153546
    Purpose
    this is signaling incompetent version of LIC-Z where all Tyrosine residues in ITAMs are mutated to leucine
    Depositor
    Katharina Gaus
    Article
    Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:
    Insert
    human TCR ζ-Chain (CD247 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    with 6 Y in 3 ITAMs mutated to L
    Promoter
    CMV
    Available since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    153546-plasmid-map-sequence-id-305909

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  20. pET-30a(+)-N-SH2

    Plasmid
    #111274
    Purpose
    express murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)
    Depositor
    Carol Post
    Article
    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5
    Insert
    murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
    Use
    Tags
    HHHHHHENLYFQG
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111274-plasmid-map-sequence-id-215709

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  21. GST-RP2-C86Y,P95L

    Plasmid
    #86074
    Purpose
    A bacterial expression plasmid encoding ciliary localization effected RP2 (RP2-C86Y,P95L) with N-terminus GST-tag.
    Depositor
    Lei Lu
    Article
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Insert
    RP2 (RP2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Changed cysteine-86 and proline-95 to tyrosine an…
    Promoter
    tac
    Available since
    May 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    86074-plasmid-map-sequence-id-177989

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  26. pAAV-DYRK3-2A-GFP

    Plasmid
    #118286
    Purpose
    Expresses mouse DYRK3 tagged with 2A peptide at it's C-terminus and GFP in mammalian cells.
    Depositor
    Vance Lemmon
    Article
    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5
    Insert
    dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 (Dyrk3 Mouse)
    Use
    AAV
    Tags
    2A peptide and GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV with beta global intron
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    118286-plasmid-map-sequence-id-229497

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  27. pKrox24(MapErk)Luc

    Plasmid
    #200112
    Purpose
    Luciferase reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity
    Depositor
    Pavel Krejčí
    Article
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Insert
    MapErk sequence in minimal promoter
    Use
    Tags
    Expression
    Mammalian
    Mutation
    highly modified sequence
    Promoter
    5 copies of designed MapErk sequence in minimal p…
    Available since
    June 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    200112-plasmid-map-sequence-id-399196

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  28. pDual-eGFP(Y203)

    Plasmid
    #63559
    Purpose
    Expression of the Mostaza (yellow) spectral variant of eGFP in bacteria and in mammalian cells. Used as a reporter for gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Article
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP(Y203)
    Use
    Lentiviral
    Tags
    His6 and T7
    Expression
    Bacterial and Mammalian
    Mutation
    Changed threonine at position 203 with respect to…
    Promoter
    CMV-EF1α hybrid (CEF)
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  31. pDule2-Tet2.0

    Plasmid
    #85497
    Purpose
    Plasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Blizzard et al J Am Chem Soc. 2015 Aug 19;137(32):10044
    Insert
    Tetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32G L65Q F108S Q109D D158S L162N
    Promoter
    lpp (constitutive)
    Available since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85497-plasmid-map-sequence-id-169854

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  34. pDule-Tet2.0

    Plasmid
    #85496
    Purpose
    Plasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Blizzard et al J Am Chem Soc. 2015 Aug 19;137(32):10044
    Insert
    Tetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32G L65Q F108S Q109D D158S L162N
    Promoter
    lpp (constitutive)
    Available since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85496-plasmid-map-sequence-id-169865

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Showing: 1281 - 1320 of 1648 results