Addgene

We narrowed to 3,035 results for: ALC

Showing: 641 - 660 of 3035 results

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pMJA285= pSin-TCRab-CMVp-Puro

Plasmid

#116875

Purpose

stable TCR expression in human T cells

Depositor

Marcos Alcocer

Article

Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-11

Insert

TCR (TRAV10, TRBV25-1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

deletion of EF1-a promotor

Promoter

CMV Promotor

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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Rat Alpha2 delta-1 HA pMT2

Plasmid

#58729

Purpose

expression of HA tagged rat Alpha2 delta-1 in mamalian cells

Depositor

Annette Dolphin

Article

Kadurin et al J Biol Chem. 2012 Sep 28;287(40):33554-6

Insert

alpha2 delta-1 (Cacna2d1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-Intron-CterFlag-MePCE

Plasmid

#113549

Purpose

Mammalian expression plasmid that can be used with the Flp-In T-REx system.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.Flex.NES-jRGECO1b.WPRE.SV40

Plasmid

#100855

Purpose

AAV expression of Cre recombinase-activated jRGECO1b, a red fluorescent calcium sensor protein, from the CAG promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Insert

jRGECO1b

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pD21-hgAPOAI

Plasmid

#124962

Purpose

Express ApoAI in the liver

Depositor

Kazuhiro Oka

Article

Belalcazar et al Circulation. 2003 Jun 3;107(21):2726-32.

Insert

human APOAI gene (LPA Human)

Use

Adenoviral

Tags

Expression

Mutation

Promoter

human APOAI

Available since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.Flex.NES-jRGECO1b.WPRE.SV40

Plasmid

#100856

Purpose

AAV expression of Cre recombinase-activated jRGECO1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Insert

jRGECO1b

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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DF-UAS-sspa-GCaMP6f

Plasmid

#67569

Purpose

Zebrafish expression of a short photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

sspa-GCaMP6f

Use

Zebrafish transgenesis (see :a spinal opsin contr…

Tags

Expression

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only
pBScMXT + mSlo1 MBr5/3

Plasmid

#16149

Depositor

Larry Salkoff

Article

Butler et al Science. 1993 Jul 9. 261(5118):221-4.

Insert

Mus musculus potassium large conductance calcium-activated channel (Kcnma1 Mouse brain)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

N/A

Promoter

Available since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
DF-UAS-spa-GCaMP6f

Plasmid

#67568

Purpose

Zebrafish expression of a photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

spa-GCaMP6f

Use

Zebrafish transgenesis (see :a spinal opsin contr…

Tags

HIS-tag

Expression

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only
pBScMXT + mSlo1 MBr5/23

Plasmid

#16151

Depositor

Larry Salkoff

Article

Butler et al Science. 1993 Jul 9. 261(5118):221-4.

Insert

Mus musculus potassium large conductance calcium-activated channel (Kcnma1 Mouse)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

N/A

Promoter

Available since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
Cav2.2 DomI + DomII pcDNA3

Plasmid

#206094

Purpose

expression of the N-terminus, Domain I, Domain II and the II-III loop (amino acids 1-1154) of rabbit Cav2.2 calcium channel

Depositor

Annette Dolphin

Article

Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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cacna2d1 BBS pcDNA3

Plasmid

#206106

Purpose

expression of rat alpha2delta-1 calcium channel auxillary subunit with a single Bungarotoxin Binding Site motif inserted after aa549HA

Depositor

Annette Dolphin

Article

Meyer et al Sci Rep. 2021 May 13;11(1):10256. doi: 1

Insert

cacna2d1 (Cacna2d1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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FKBP12-CB2mutx2-mTagBFP2-HAx2

Plasmid

#207147

Purpose

Tandem mutant calcium-buffer with rapalog dimerizer

Depositor

Antony Galione

Article

Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

Insert

Calbindin-2 (CALB2 Human)

Use

Tags

FKBP12, HA, and mTagBFP2

Expression

Mammalian

Mutation

E40Q, E87Q, E131Q, E175Q, E219Q

Promoter

CMV

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 pCAGGS

Plasmid

#206101

Purpose

expression of rabbit Cav2.2 calcium channel, the 3'UTR is deleted, this enhances the expression

Depositor

Annette Dolphin

Article

Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV/B-actin

Available since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 DomI pMT2

Plasmid

#206127

Purpose

expression of the N-terminus, Domain I and the I-II loop (amino acids 1-483) of rabbit Cav2.2 calcium channel

Depositor

Annette Dolphin

Article

Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ad MLP/TPL/SV40

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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cacna2d1 BBS pMT2

Plasmid

#206062

Purpose

expression of rat calcium channel alpha2delta-1 with a single Bungarotoxin Binding Site motif inserted after aa549

Depositor

Annette Dolphin

Article

Meyer et al Sci Rep. 2021 May 13;11(1):10256. doi: 1

Insert

cacna2d1 (Cacna2d1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ad MLP/TPL/SV40

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pEE041

Plasmid

#176798

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

410

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMJA289= pSin-EF2-TRAV10-P2A-TRBV25-Puro

Plasmid

#116876

Purpose

stable TCR expression in human T cells

Depositor

Marcos Alcocer

Article

Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-11

Insert

TRAV10 - P2A - TRBV25 (TRAV10, TRBV25-1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-PinkyCaMP

Plasmid

#232856

Purpose

An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under hSyn promoter

Depositor

Olivia Masseck

Article

Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

Insert

PinkyCaMP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-PinkyCaMP

Plasmid

#232858

Purpose

An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under CaMKII promoter

Depositor

Olivia Masseck

Article

Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

Insert

PinkyCaMP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CaMKII

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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