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We narrowed to 975 results for: Cerulean

Showing: 641 - 660 of 975 results
  1. pcDNA3-PKAsub(T/A)-YPet-NES

    Plasmid
    #138205
    Purpose
    Encodes negative-control substrate fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.
    Depositor
    Insert
    PKAsub(T/A)-YPet-NES
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  2. pcDNA3-PKAsub-YPet-Kras

    Plasmid
    #138206
    Purpose
    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (bimAKAR); plasma membrane targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.
    Depositor
    Insert
    PKAsub-YPet-Kras
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  3. pcDNA3-Cyto-CaNAR2

    Plasmid
    #64729
    Purpose
    Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CaNAR2-NES
    Tags
    Cerulean3, Nuclear export signal (NES), and YPet
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  4. pcDNA3.1-KozATG-dL5-2XG4S-mCer3

    Plasmid
    #73207
    Purpose
    Expresses dL5(E52D)-mCer3 fusion protein in cytosol of mammalian cells, penetrates nucleus. (MBIC5, dL5**, FAP)
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KozATG-dL5-2XG4S-mCer3
    Tags
    The FAP and mCerulean3 are fused with 2 copies of…
    Expression
    Mammalian
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    CMV
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  5. mCer-TG2-YFP

    Plasmid
    #85471
    Purpose
    TG2 biosensor that allows for quantitative assessment of TG2 conformational changes in live cells using FRET
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hTG2 (TGM2 Human)
    Tags
    EYFP and mCerulean
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  6. pROF346

    Plasmid
    #155331
    Purpose
    Binary vector for expression of PULSE system (under the control of AtUbi10 promoter) controlling Venus-H2B. It constitutively expresses Cerulean-NLS and a plant selection cassette.
    Depositor
    Insert
    RB_KanR_Tnos-NLS-PIF6-E-PUbi10_Tnos-nCerulean-PUbi10_Tnos-NLS-VP16-PhyB-PUbi10_Tnos-EL222-NLS-SRDX-PUbi10_T35S-nVenus-POpto_LB
    Use
    Synthetic Biology
    Expression
    Plant
    Available since
    Oct. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. pcDNA3.1 HA CFP hNKCC1 WT (NT15-H)

    Plasmid
    #49077
    Purpose
    Expresses human NKCC1 with an N-terminal 3xHA-CFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Tags
    3x HA and mCerulean
    Expression
    Mammalian
    Mutation
    262 silent mutations from native hNKCC1
    Promoter
    CMV
    Available since
    Nov. 19, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  8. pLenti.CAG.H2B-cerFP.W.SINloxP

    Plasmid
    #51006
    Purpose
    Lentivector that expresses H2B-cerFP from internal CAG enhancer-promoter
    Depositor
    Insert
    H2B-cerFP
    Use
    Lentiviral
    Tags
    histone 2B
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 2, 2014
    Availability
    Academic Institutions and Nonprofits only
  9. GEF-H1 FLARE212.pa

    Plasmid
    #134930
    Purpose
    GEF-H1 biosensor that does not have GEF activity.
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GEF-H1(1-212)-yPet-4X linker-Cerulean3-GEF-H1(213-end)
    Tags
    None.
    Expression
    Bacterial and Mammalian
    Promoter
    CMV and T7
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  10. pcDNA3-PM-CaNAR2

    Plasmid
    #64730
    Purpose
    Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lyn-CaNAR2
    Tags
    Cerulean3, N-terminal targeting sequence from Lyn…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  11. pcDNA3.1-COXIV-COX8-dL5-2XG4S-mCer3

    Plasmid
    #73208
    Purpose
    Expresses dL5(E52D)-mCer3 fusion protein in mitochondria of mammalian cells. (MBIC5, dL5**, FAP)
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    COXIV-COX8-dL5-2XG4S-mCer3 (COX8A Human, Synthetic)
    Tags
    The FAP and mCerulean3 are fused with 2 copies of…
    Expression
    Mammalian
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    CMV
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  12. pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3

    Plasmid
    #73205
    Purpose
    Expresses myc-dL5(E52D)-mCer3 fusion protein in nuclei of mammalian cells. (MBIC5, dL5**, FAP)
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS-myc-dL5-2XG4S-mCer3 (MYC Human, Synthetic)
    Tags
    The FAP and mCerulean3 are fused with 2 copies of…
    Expression
    Mammalian
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    CMV
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 641 - 660 of 975 results