Skip to main content
Addgene
Showing: 641 - 660 of 6240 results

  1. GTL-c

    Plasmid
    #81098
    Purpose
    Gene Tagging vector LEU/CFP - Plasmid for yeast enhanced monomeric CFP (yemCFP) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)
    Depositor
    Serge Pelet
    Article
    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231
    Insert
    yemCFP
    Use
    Cre/Lox
    Tags
    yemCFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    81098-plasmid-map-sequence-id-164508

    Enlarge

  2. GTT-c

    Plasmid
    #81108
    Purpose
    Gene Tagging vector TRP/CFP - Plasmid for yeast enhanced monomeric CFP (yemCFP) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)
    Depositor
    Serge Pelet
    Article
    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231
    Insert
    yemCFP
    Use
    Cre/Lox
    Tags
    yemCFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    81108-plasmid-map-sequence-id-164588

    Enlarge

  3. pLVTHM

    Plasmid
    #12247
    Purpose
    2nd gen lentivector expressing shRNA from H1 promoter and GFP. For direct cloning of shRNA or sub-cloning of an H1-shRNA cassette from pSUPER. Dox-controllable if used with tTRKRAB expressing plasmid
    Depositor
    Didier Trono
    Article
    Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12247-plasmid-map-sequence-id-352425

    Enlarge

  5. pMuLE ENTR CMV loxP L5-L2

    Plasmid
    #62094
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and multiple cloning site flanked by loxP sites. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    Cre/Lox; Mule gateway entry vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62094-plasmid-map-sequence-id-101383

    Enlarge

  6. pSicoR-mCh-empty

    Plasmid
    #21907
    Purpose
    Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression.
    Depositor
    Miguel Ramalho-Santos
    Article
    Gaspar-Maia et al Nature. 2009 Jul 8. ():.
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 30, 2009
    Availability
    Academic Institutions and Nonprofits only
    21907-plasmid-map-sequence-id-246205

    Enlarge

  7. EC71174

    Plasmid
    #154067
    Purpose
    Level 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPER
    Depositor
    Cristobal Uauy
    Article
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Inserts
    HS Cre recombinase
    Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
    HYGROMYCIN Resistance Cassette
    Use
    Cre/Lox and Synthetic Biology; Golden gate
    Tags
    Expression
    Bacterial and Plant
    Mutation
    The Arabidopsis thaliana U5 small nuclear ribonuc…
    Promoter
    35S promoter, Hordeum vulgare HSP 17 promoter use…
    Available since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    154067-plasmid-map-sequence-id-313091

    Enlarge

  10. hSyn-DIO-somBiPOLES-eGFP

    Plasmid
    #192584
    Purpose
    AAV-vector for bidirectional optogenetic manipulation of Cre-positive neurons
    Depositor
    Simon Wiegert
    Insert
    somBiPOLES
    Use
    AAV
    Tags
    soma-targeting motif from Kv2.1 channel
    Expression
    Mammalian
    Mutation
    Promoter
    human synapsin
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    192584-plasmid-map-sequence-id-379290

    Enlarge

  11. pDD288

    Plasmid
    #137017
    Purpose
    mNG-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Bob Goldstein, Daniel Dickinson
    Insert
    mNG-GLO^SEC^3xFlag + ccdB
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans GLO-optimized mNG
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137017-plasmid-map-sequence-id-274039

    Enlarge

  12. pDD291

    Plasmid
    #137019
    Purpose
    mKate2-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Bob Goldstein, Daniel Dickinson
    Insert
    mKate2-GLO^SEC^3xFlag + ccdB
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans GLO-optimized mKate2
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137019-plasmid-map-sequence-id-274037

    Enlarge

  13. pDD316

    Plasmid
    #137020
    Purpose
    mTurq2-GLO^SEC^2xHA vector with ccdB markers for cloning homology arms
    Depositor
    Bob Goldstein, Daniel Dickinson
    Insert
    mTurq2-GLO^SEC^2xHA + ccdB
    Use
    CRISPR and Cre/Lox
    Tags
    2xHA and C. elegans GLO-optimized mTurq2
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137020-plasmid-map-sequence-id-274032

    Enlarge

  14. pDD289

    Plasmid
    #137018
    Purpose
    GFP-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Bob Goldstein, Daniel Dickinson
    Insert
    GFP-GLO^SEC^3xFlag + ccdB
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans GLO-optimized GFP
    Expression
    Worm
    Mutation
    Promoter
    Available since
    April 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    137018-plasmid-map-sequence-id-291422

    Enlarge

  16. pAAV-nEF-Con/Foff 2.0-ChR2(ET/TC)-EYFP

    Plasmid
    #137140
    Purpose
    Intersectional viral expression of ChR2(ET/TC)-EYFP in cells expressing Cre AND NOT Flp
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Has Service
    AAV8
    Insert
    Con/Foff 2.0-ChR2(ET/TC)-EYFP
    Use
    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt
    Tags
    Expression
    Mutation
    E123T, T159C
    Promoter
    nEF
    Available since
    June 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    137140-plasmid-map-sequence-id-272725

    Enlarge

  18. pBCSK_LEU2_I

    Plasmid
    #109060
    Purpose
    yeast shuttle vector for insertion into yeast chromosomes in pBCSK backbone. LoxP sites upstream of LEU2. For teaching purposes
    Depositor
    Colin Logie
    Insert
    LEU2 (LEU2 Zygosaccharomyces rouxii, Budding Yeast)
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    LEU2
    Available since
    Aug. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    109060-plasmid-map-sequence-id-209546

    Enlarge

  19. CAG-loxP-3xpA-loxP-sfGFP+pCI syntron

    Plasmid
    #172478
    Purpose
    sfGFP with a synthetic intron from the pCI expression vector (Promega)
    Depositor
    Mario Capecchi
    Insert
    sfGFP
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172478-plasmid-map-sequence-id-338912

    Enlarge

Showing: 641 - 660 of 6240 results