We narrowed to 72,194 results for: LAS

1. pLEX-FKBP-Rab5-Blasticidin

   Plasmid

   #120715

   Purpose

   Expresses early endosome-targeted recruiter protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   Rab5a
   

   Use

   Lentiviral

   Tags

   FKBP and mCherry

   Expression

   Mammalian

   Mutation

   Amino acids 2-215

   Promoter

   CMV

   Available since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXS-IRES-BLAST co hPHGDH

   Plasmid

   #154915

   Purpose

   Express codon optimized human PHGDH

   Depositor

   Richard Possemato

   Article

   Richard et al Richard Possemato Lab plasmids

   Insert

   PHGDH, codon optimized (PHGDH Synthetic, Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Codon optimized for expression in human cells

   Promoter

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Endoplasmic reticulum-targeted optodPLD

   Plasmid

   #140059

   Purpose

   Encodes CRY2-mCherry-dPLD-P2A-CIBN-Sac1(TM) for mammalian expression

   Depositor

   Jeremy Baskin

   Article

   Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Phospholipase D
   

   Use

   Tags

   CIBN, CRY2, P2A, Sac1(TM), and mCherry

   Expression

   Mammalian

   Mutation

   H170A (catalytic dead)

   Promoter

   Available since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. U6-sgR26-eCas9-T2A-BlastR

   Plasmid

   #172493

   Purpose

   For CRISPR-assisted HDR, Rosa26 locus

   Depositor

   Tyler Jacks

   Article

   Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

   Insert

   eCas9, BlastR and sgRNA targeting the Rosa26 (R26) safe harbor locus
   

   Use

   CRISPR, Lentiviral, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. p2T-CMV-eA3AmaxNG-BlastR

   Plasmid

   #152999

   Purpose

   C-to-T base editor, NG PAM

   Depositor

   David Liu

   Article

   Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

   Insert

   eA3A-nSpCas9 NG-UGI-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. aMHC-Puro Rex-Blast

   Plasmid

   #21231

   Depositor

   Mark Mercola

   Article

   Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

   Insert

   alpha myosin heavy chain promoter
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-CMV-Blast-DNFGFR1-HA

   Plasmid

   #130887

   Purpose

   Expresses dominant negative mutant mouse FGFR1

   Depositor

   Gian-Paolo Dotto

   Article

   Bordignon et al Cell Rep. 2019 Aug 27;28(9):2358-2372.e6

   Insert

   Fgfr1 (Fgfr1 Mouse)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   Dominant Negative receptor lacking cytosolic cata…

   Promoter

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-dSaCas9-KRAB-blast

   Plasmid

   #191654

   Purpose

   Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain for gene silencing and the blasticidin resistance gene.

   Depositor

   Moshe Szyf

   Article

   Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

   Insert

   dSaCas9-KRAB
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCLIPf-NK1R Control Plasmid

   Plasmid

   #101125

   Purpose

   Expression of CLIP-tagged Neurokinin-1 Receptor in Mammalian cells

   Depositor

   Ana Egana, New England Biolabs

   Insert

   Neurokinin-1 Receptor, Truncated (TACR1 Human)
   

   Use

   Tags

   10xHis, CLIP-tag (CLIPf), and FLAG Epitope

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 27, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pLIX403-EBFP2-ccdB-Blast

    Plasmid

    #158557

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti X2 Blast/shp16 (w112-1)

    Plasmid

    #22261

    Depositor

    Eric Campeau

    Insert

    sh cyclin-dependent kinase inhibitor 2A
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 15, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXS-IRES-Blast CHKA

    Plasmid

    #218540

    Purpose

    human CHKA cDNA

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    CHKA (CHKA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pscALPS gag-gfp Blasti

    Plasmid

    #115803

    Purpose

    Express gag-gfp fusion driven by SFFV promoter

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    gag-gfp
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSG81-pLas-tetO-deGFPssrA

    Plasmid

    #102454

    Purpose

    Expresses deGFP fluorescent protein with ssrA degradation tag in E. coli. The expression is controlled by a pLas-tetO combinatorial promoter.

    Depositor

    Richard Murray

    Article

    Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

    Insert

    deGFP
    

    Use

    Tags

    ssrA

    Expression

    Bacterial

    Mutation

    Promoter

    pLas-tetO

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. U6-sgfiller-eCas9-T2A-BlastR

    Plasmid

    #172437

    Purpose

    Expresses eCas9, BlastR and sgRNA cloning site

    Depositor

    Tyler Jacks

    Article

    Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

    Inserts

    eCas9
    BlastR
    

    Use

    CRISPR, Lentiviral, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p2T-CMV-BE4max-BlastR

    Plasmid

    #152991

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    BE4-nSpCas9-UGI-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. miniTol2 x EF1a_EKAREN5 + PGK-blast

    Plasmid

    #167818

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5
    

    Use

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMXS-IRES-Blast ACSL4

    Plasmid

    #173164

    Purpose

    Retroviral vector to express sgRNA resistant human ACSL4

    Depositor

    Kivanc Birsoy

    Article

    Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

    Insert

    Acyl-CoA Synthetase Long Chain Family Member 4 (ACSL4 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Synonymous mutations at sgRNA sites

    Promoter

    MMLV

    Available since

    Aug. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pf CAG mc2 IRES blast

    Plasmid

    #206235

    Purpose

    For expressing cloned exons as circular RNA.

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMCP-GPI Control Plasmid

    Plasmid

    #101132

    Purpose

    Expression of MCP-Tagged GPI anchor in Mammalian cells

    Depositor

    Ana Egana, New England Biolabs

    Insert

    GPI
    

    Use

    Tags

    MCP-tag (Mutant ACP- tag)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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