We narrowed to 4,737 results for: p2a

1. JDW 1050 (pME_V5_GATA1_P2A_H2A_mCherry)

   Plasmid

   #232127

   Purpose

   A middle entry gateway clone containing V5 tagged human GATA1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   GATA1 (GATA1 Human)
   

   Use

   Gateway cloning

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBT2-4xUAS:P2A-EGFP

   Plasmid

   #127591

   Purpose

   This vector is used for cloning a gene driven by 4xnrUAS, to be co-expressed with EGFP through P2A self-cleaving peptides.

   Depositor

   Longhou Fang

   Article

   Gu et al Science. 2019 Mar 8;363(6431):1085-1088.

   Type

   Empty backbone

   Use

   Zebrafish expression

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. FUG-P2A-tC3-W

   Plasmid

   #129475

   Purpose

   3rd-generation lentiviral vector for hUbC-driven co-expression of EGFP and truncated C3

   Depositor

   Robert Gross

   Insert

   truncated (AA173-201) exoenzyme C3 from C. botulinum, P2A, EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human ubiquitin C

   Available since

   Feb. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. p3E p2a-tomato

   Plasmid

   #135211

   Purpose

   Red fluorescent tag

   Depositor

   David Tobin

   Article

   Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

   Insert

   tdTomato
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3E p2a-turquoise2

   Plasmid

   #135213

   Purpose

   Cyan flourescent tag

   Depositor

   David Tobin

   Insert

   mTurquoise2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. p3E-P2A-GFP no-pA

   Plasmid

   #80826

   Purpose

   P2A followed by GFP without pA

   Depositor

   Philip Washbourne

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   p3E-P2A-GFP no-pA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti_EGFP-P2A-HAUS6_IRES_Blast

   Plasmid

   #182886

   Purpose

   Transfer vector for production of lentivirus. Expresses EGFP-P2A-HAUS6 (WT full length)

   Depositor

   Daniel Gerlich

   Article

   David et al J Cell Biol. 2019 Jul 1;218(7):2150-2168

   Insert

   HAUS 6 (HAUS6 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1alpha core

   Available since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME-memGFP-P2A

   Plasmid

   #80809

   Purpose

   Fyn myristoylation domain fused to N-terminus of GFP (memGFP) without stop codon, followed by P2A

   Depositor

   Philip Washbourne

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   pME-memGFP-P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. nCas9-SpRY_P2A-mCherry

   Plasmid

   #219935

   Purpose

   Mammalian expression of SpRY-nCas9 with P2A-mCherry marker for use with MOBE systems

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   Sp-nCas9(D10A)-SpRY_P2A-mCherry
   

   Use

   CRISPR

   Tags

   SV40 bpNLS and SV40 bpNLS-P2A-mCherry

   Expression

   Mammalian

   Mutation

   nCas9(D10A)

   Promoter

   CMV

   Available since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. p3E-P2A-mKate2 no-pA

    Plasmid

    #80828

    Purpose

    P2A followed by mKate2 without pA

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    p3E-P2A-mKate2 no pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. LV_EF1a_MAPK14-P2A-Hygro_Barcode

    Plasmid

    #170239

    Purpose

    Barcoded lentiviral vector to express MAPK14 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    MAPK14 (MAPK14 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pME-nlsGFP-P2A

    Plasmid

    #80808

    Purpose

    nuclear localization signal fused to N-terminus of GFP (nlsGFP) without stop codon, followed by P2A

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    minimal cytomegalovirus immediate early enhancer/promoter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDRESS_mTurquoise2_spatial-linker-P2A_mScarletI

    Plasmid

    #130510

    Purpose

    expresses mScarlet1

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Protoc. 2020 Feb;15(2):450-478. doi:

    Insert

    mScarlet1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. LV_EF1a_CDK4-P2A-Hygro_Barcode

    Plasmid

    #170223

    Purpose

    Barcoded lentiviral vector to express CDK4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    CDK4 (CDK4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. nCas9-NG_P2A-mCherry

    Plasmid

    #219934

    Purpose

    Mammalian expression of Sp-nCas9-NG with P2A-mCherry marker for use with MOBE systems

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    Sp-nCas9(D10A)-NG_P2A-mCherry
    

    Use

    CRISPR

    Tags

    SV40 bpNLS and SV40 bpNLS-P2A-mCherry

    Expression

    Mammalian

    Mutation

    nCas9(D10A)

    Promoter

    CMV

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. CMV:eGFP-p2A-HA-GPR151

    Plasmid

    #190133

    Purpose

    expresses eGFP-p2A-HA-GPR151 (wild-type variant) in mammalian cells

    Depositor

    Joshua Knowles

    Article

    Bielczyk-Maczynska et al Nat Commun. 2022 Dec 1;13(1):7408. doi:

    Insert

    eGFP-p2A-HA-GPR151
    

    Use

    Lentiviral

    Tags

    HA, eGFP, and p2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. th-P2A-Gal4 donor

    Plasmid

    #65563

    Purpose

    Intron targeting-mediated Gal4 knockin at the zebrafish th locus.

    Depositor

    Jiu-lin Du

    Article

    Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

    Insert

    th-P2A-Gal4 (th Zebrafish)
    

    Use

    Zebrafish injection

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-P2A-CIAR_pLenti_CMV_Puro

    Plasmid

    #86503

    Purpose

    Lenti plasmid for generating CIAR expressing stable cell lines

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    GFP-P2A-CIAR (SOS1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    T968L in SOScat

    Promoter

    CMV

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. p3E-P2A-CFP no-pA

    Plasmid

    #80827

    Purpose

    P2A followed by CFP without pA

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    p3E-P2A-CFP no pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pH2R-P2A-mCherry-N1

    Plasmid

    #84331

    Purpose

    Co-expresses (untagged) Histamine-2 receptor and mCherry

    Depositor

    Dorus Gadella

    Article

    van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

    Insert

    H2R-P2A-mCherry (HRH2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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