We narrowed to 16,590 results for: NUC

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pHR-U6-sgTel-CMV-puro-P2A-mCherry-TRF1

Plasmid

#169449

Purpose

expression of TRF1-mcherrry and sgRNA targeting telomeres

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

mCherry-TRF1

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pSB1K3_M13ps_LacZα_gIII

Plasmid

#190435

Purpose

M13 packaging signal, LacZα gene, and M13 gIII gene (also called gp3, or g3p) cloned into the pSB1K3 vector.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

M13 packaging signal
M13 gIII
LacZα

Use

Synthetic Biology

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro2-H2B-iRFP

Plasmid

#197352

Purpose

Encoding nuclear-targeted iRFP713.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

iRFP with nucleus-targeting sequence

Use

Lentiviral

Tags

H2B (human histone 2B)

Expression

Mammalian

Promoter

EF-1α

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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SlugCas9

Plasmid

#163793

Purpose

Expresses SlugCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET22-HT/plsA

Plasmid

#73625

Purpose

Produces Escherichia coli adenylate kinase (PlsA), N-terminal His6 tag

Depositor

T. J. Kappock, James Williamson

Article

Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

Insert

adenylate kinase (adk Escherichia coli str. K-12 substr. MG1655)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-dCas9 VP64-T2A-GFP

Plasmid

#53192

Purpose

Co-expresses human optimized S. pyogenes dCas9-VP64 and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized dead Cas9 VP64 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D10A and H840A

Promoter

hUbC

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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His-CL7-2NLS-SpyCas9(dS)-A22p3star

Plasmid

#222970

Purpose

Bacterial expression of A22p-fused SpyCas9 construct

Depositor

Jennifer Doudna

Article

Chen et al Nat Commun. 2024 Feb 26;15(1):1727. doi:

Insert

His-CL7-2NLS-SpyCas9(dS)-A22p3

Use

CRISPR

Tags

CL7 and His

Expression

Bacterial

Promoter

tac

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pmScarlet_NES_C1

Plasmid

#85060

Purpose

In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

NES

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pEn-Chimera

Plasmid

#61432

Purpose

Gateway-Entry vector containing AtU6-26 promoter and sgRNA backbone

Depositor

Holger Puchta

Article

Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

Insert

AtU6-26:sgRNA

Use

CRISPR

Expression

Plant

Promoter

AtU6-26

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pRc/RSV-m CBP-HA

Plasmid

#16701

Depositor

Richard Goodman

Article

Chrivia et al Nature. 1993 Oct 28. 365(6449):855-9.

Insert

CBP (Crebbp Mouse)

Tags

HA

Expression

Mammalian

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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pHBV-Luc

Plasmid

#71414

Purpose

To see the activity of HBV transcription using Luciferase assay

Depositor

Wang-Shick Ryu

Article

Cha et al J Gen Virol. 2009 Apr;90(Pt 4):978-86. d

Insert

HBV core promoter, enhancer I, enhancer II and luciferase

Use

Luciferase

Expression

Mammalian

Promoter

HBV

Available Since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pET30a-GlmU-NahK-ET64

Plasmid

#234663

Purpose

Expression an ELP-tagged fusion enzyme of GlmU and NahK

Depositor

Xianwei Liu

Article

Wu et al Chembiochem. 2025 Jan 13:e202401005. doi

Inserts

N-acetylglucosamine- 1-phosphate uridyltransferase
N-acetylhexosamine 1-kinase

Tags

ELP

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCEFLmGFP-Gli2

Plasmid

#37672

Depositor

Philip Beachy

Article

Kim et al Proc Natl Acad Sci U S A. 2009 Dec 22;10

Insert

Gli2 (Gli2 Mouse)

Tags

mGFP

Expression

Mammalian

Promoter

EF1a

Available Since

July 10, 2012

Availability

Academic Institutions and Nonprofits only

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pDESTmycQKI

Plasmid

#19870

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

quaking homolog, KH domain RNA binding (QKI Human)

Tags

MYC

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only
2HA-pDEST-N

Plasmid

#118373

Purpose

Gateway compatible mammalian expression vector with N-terminal 2HA to tag proteins of interest.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

2HA

Expression

Mammalian

Available Since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(CCG3)

Plasmid

#235074

Purpose

Expresses Tad-CBE(CCG3) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(CCG3)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pDe-CAS9

Plasmid

#61433

Purpose

Binary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequence

Depositor

Holger Puchta

Article

Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

Insert

Cas9

Use

CRISPR

Expression

Plant

Promoter

PcUBI4-2

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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single cut NH-donor

Plasmid

#83575

Purpose

eGFP reporter donor containing single SA cutting site for NHEJ knock-in

Depositor

Bo Feng

Article

He et al Nucleic Acids Res. 2016 May 19;44(9):e85

Insert

eGFP

Use

Nhej donor

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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FRP1642_PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

Plasmid

#58442

Purpose

Cassette to replace yeast promoters with an inducible promoter containing 4 lexA boxes

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

Expression

Yeast

Promoter

PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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TR-Csy4-H29A

Plasmid

#80602

Purpose

Expresses Csy4 with an inactivating H29A mutation from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Article

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

Csy4-H29A

Use

AAV

Expression

Mammalian

Mutation

His29 mutated to Ala

Promoter

CBA

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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