We narrowed to 10,348 results for: poly

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pCMV-SEMPER-mARG_ACC-gvpA_ACC-gvpNJKFGW-EmGFP_IRES-mCherry

Plasmid

#221080

Purpose

SEMPER plasmid for expression of gas vesicles using mammalian acoustic reporter genes (mARGs)

Depositor

Mikhail Shapiro

Article

Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

Insert

ACC-gvpA_ACC-gvpNJKFGW-EmGFP_IRES-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pRK172 asyn(1-103)

Plasmid

#240597

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

Ni et al J Mol Biol. 2019 Sep 6;431(19):3913-3919

Insert

aSyn (SNCA Human)

Expression

Bacterial

Mutation

aa 1-103 only

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINB-OTULIN (full-length, aa 1-352)

Plasmid

#61464

Purpose

Expresses human OTULIN (full-length) in E. coli.

Depositor

David Komander

Article

Keusekotten et al Cell. 2013 Jun 6;153(6):1312-26. doi: 10

Insert

OTULIN (OTULIN Human)

Tags

His6-3C

Expression

Bacterial

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pRK172 asyn(1-122)

Plasmid

#240598

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

Ni et al J Mol Biol. 2019 Sep 6;431(19):3913-3919

Insert

aSyn (SNCA Human)

Expression

Bacterial

Mutation

aa 1-122 only

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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HotPETase_pET21b

Plasmid

#215407

Purpose

Expression construct for HotPETase gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

HotPETase

Expression

Bacterial

Mutation

S121E/D186H/R280A/P181V/S207R/S214Y/Q119K/S213E/N…

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-PBRM1-pgk-puro

Plasmid

#85746

Purpose

tetracyline-inducible lentiviral vector expressing human polybromo-1 (PBRM1) in mammlian cells

Depositor

Emily Dykhuizen

Article

Chowdhury et al PLoS One. 2016 Apr 21;11(4):e0153718. do

Insert

PBRM1 (PBRM1 Human)

Use

Lentiviral

Tags

none

Promoter

CMV

Available Since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

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pSAM_AraC

Plasmid

#91569

Purpose

Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.

Depositor

Harry Mobley

Article

Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.

Insert

AraC/PBAD

Expression

Bacterial

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 wt

Plasmid

#120313

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV-mir-17-92

Plasmid

#64100

Purpose

expresses mir-17-92

Depositor

Lin He

Article

Olive et al Elife. 2013 Oct 15;2:e00822. doi: 10.755

Insert

miR-17-92 (MIR17HG Human)

Expression

Mammalian

Promoter

MSCV

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-EGFP

Plasmid

#177136

Purpose

Bacterial vector for expression of an N-terminal GST fusion of EGFP with a TEV protease site located between the GST tag and EGFP

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

EGFP

Expression

Bacterial

Promoter

Tac

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 YFP

Plasmid

#84910

Purpose

expression of YFP in mammalian cells

Depositor

Aaron Gitler

Article

Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

Insert

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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TDP43-NTD

Plasmid

#241024

Purpose

Bacterial expression of TDP-43 NTD

Depositor

Nicolas Fawzi

Article

Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

TDP43-NTD

Tags

6x His

Expression

Bacterial

Mutation

changed N81 to stop codon

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS_NP_EBOV

Plasmid

#103049

Purpose

Encodes Ebola virus NP gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

Depositor

Elke Mühlberger

Article

Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

Insert

NP gene of Ebola virus (NP Zaire ebolavirus, Mayinga)

Expression

Mammalian

Mutation

silent mutation (T to C) at position 928 of NP ge…

Promoter

CAG

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-dCas13b-ADAR2DD

Plasmid

#190913

Purpose

Addgene #103871 with 3x FLAG on N-terminal of dCas13

Depositor

Timothy Jarome

Article

Farrell et al Mol Psychiatry. 2023 May 17. doi: 10.103

Insert

3X FLAG

Use

CRISPR

Tags

3X FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pLIB-HIS-TEV-Cyclin-B

Plasmid

#177012

Purpose

Generate baculovirus for insect cell expression of Cyclin-B with N-terminal Polyhistidine-TEV

Depositor

Andrea Musacchio

Article

Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

Insert

Cyclin B1 (CCNB1 Human)

Tags

Polyhistidine-TEV

Expression

Insect

Mutation

codon-optimised for insect cell expression

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pN3-MGA

Plasmid

#107715

Purpose

Expression plasmid for 3xFLAG-tagged MGA

Depositor

Guntram Suske

Article

Stielow et al PLoS Genet. 2018 Jan 30;14(1):e1007193.

Insert

MGA (MGA Human)

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pET19-TdTwt

Plasmid

#207466

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

Depositor

Jay Keasling

Article

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

TdTwt (Dntt Mouse)

Tags

10xHis

Expression

Bacterial

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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FUS_anion

Plasmid

#98656

Purpose

Expresses untagged FUS AA 1-163

Depositor

Nicolas Fawzi

Article

Burke et al Mol Cell. 2015 Oct 15;60(2):231-41. doi:

Insert

FUS (FUS Human)

Expression

Bacterial

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1_WT RAD23B

Plasmid

#201457

Purpose

Expresses human RAD23B with an N-terminal GST tag in E. coli

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Tags

GST

Expression

Bacterial

Mutation

Coding sequence has been optimised for expression…

Promoter

tac promoter

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pSR657

Plasmid

#65214

Purpose

Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cells

Depositor

Robert Kotin

Article

Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

Inserts

modified AAV-2 rep78
modified AAV-2 cap

Use

AAV

Expression

Insect

Promoter

p10 and polyhedrin

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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