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pBS-PtH2B

Plasmid

#133390

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

Depositor

Blair Madison

Publication

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Expression

Mammalian

Promoter

No promoter

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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BII-CPspaH2B

Plasmid

#133385

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

Depositor

Blair Madison

Publication

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Expression

Mammalian

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pICE ADE2-HphMx

Plasmid

#122895

Purpose

ADE2-HphMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Oct. 13, 2019

Availability

Academic Institutions and Nonprofits only

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LRG/Foxa1 e2.4

Plasmid

#105511

Purpose

Lentiviral expression of Foxa1 DNA-binding domain (exon 2) targeting sgRNA

Depositor

Christopher Vakoc

Publication

Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

Insert

Foxa1 (sgRNA, e2.4)

Use

Lentiviral

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pEN_TmiRc3_shNT

Plasmid

#81067

Purpose

Gateway entry vector encoding non-targeting shRNA

Depositor

Darren Saunders

Publication

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

non-targeting shRNA

Use

Cloning

Promoter

N/A

Available since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pNR276

Plasmid

#79577

Purpose

TP901 attB-attP. GFP will express if sites recombine.

Depositor

Timothy Lu

Publication

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pFUPI

Plasmid

#80279

Purpose

A bicistronic lentiviral vector used to stably express cDNAs.

Depositor

Jeremy Luban

Publication

Neagu et al J Clin Invest. 2009 Oct;119(10):3035-47.

Insert

Puro resistance cassette, IRES, cloning site

Use

Lentiviral

Expression

Mammalian

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pNR239

Plasmid

#79576

Purpose

A118 attB-attP. GFP will express if sites recombine.

Depositor

Timothy Lu

Publication

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pET29b-ClbP

Plasmid

#48244

Purpose

Untagged, full-length protein for in vivo peptidase assays

Depositor

Emily Balskus

Publication

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbP

Tags

None

Expression

Bacterial

Promoter

T7

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGGF003

Plasmid

#48844

Purpose

Provides a D-alanine resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Publication

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pMAS::D-AlanineR::tMAS

Use

Golden gate compatible cloning vector

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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ABEx1_SpRY_V106W (ABE8e_V28C_nCas9_SpRY_V106W)

Plasmid

#251552

Purpose

Mammalian expression plasmid encoding an nCas9-SpRY_V106W adenine base editor (ABE8e) carrying the V28C mutation in the deaminase domain

Depositor

George Church

Publication

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_V28C-nCas9_SpRY_V106W

Use

CRISPR

Expression

Mammalian

Mutation

ABEx4 V28C_M151E

Available since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-15nmEML-mRFP

Plasmid

#231177

Purpose

Stabilizes the ER-mitochondrial distance at 15 nm; tagged with mRFP

Depositor

Dmitry Lim

Publication

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

15nmEML-mRFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-10nm-EBFP2

Plasmid

#231184

Purpose

Expresses a synthetic linker, tagged with EBFP2, Stabilizing the ER-mitochondrial distance at 10 nm

Depositor

Dmitry Lim

Publication

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

10nm-EBFP2

Expression

Mammalian

Promoter

CMV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLV-hCDC6p-Venus

Plasmid

#212665

Purpose

Express Venus under CDC6 promoter

Depositor

Yumi Konagaya, Tobias Meyer

Publication

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

hCDC6p-Venus

Use

Lentiviral

Promoter

hCDC6p

Available since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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p15A-OptoCre-knt-P-R*

Plasmid

#189852

Purpose

Cre-inducible activation of knt for kanamycin resistance.

Depositor

Mary Dunlop

Publication

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Insert

P-loxP-TT-loxP-R*-knt

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

P (T7 phage gene 10)

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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saposin B^W

Plasmid

#161759

Purpose

Saposin B (Homo Sapien) containing a single mutation, arginine 38 to tryptophan

Depositor

Sam Gellman

Publication

Kurgan et al Biochemistry. 2021 Apr 13;60(14):1108-11

Insert

saposin B R38_W

Tags

His-tag

Expression

Bacterial

Mutation

changed Arginine 38 to Tryptophan

Promoter

T7

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pSB_init-Sb66

Plasmid

#159422

Purpose

Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb66

Depositor

Dianfan Li

Publication

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

Sb66

Tags

c-Myc and poly-His

Expression

Bacterial

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX303-ARF6-mNeonGreen

Plasmid

#162027

Purpose

Expression of tagged ARF WT

Depositor

David Bryant

Publication

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

ARF6 (ARF6 Human)

Use

Lentiviral

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pENTR-R2L3-pA-floxPGKneopA

Plasmid

#139543

Purpose

Provides pA sequences N-terminal to floxed PGK-neo-pA cassette on Gateway entry vector

Depositor

Minoru Ko

Insert

Neomycin resistant gene

Expression

Mammalian

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pAV0714

Plasmid

#133479

Purpose

Expression in fission yeast from promoter pact1, integrated at ura4 locus

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(act1)-sfGFP-terminator(ScCYC1)

Expression

Yeast

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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