We narrowed to 38,770 results for: CMV

1. plx208-CMV-Signal seuqence-enkephalin-CapC-FLAG-μOR-NNES-SmBit

   Plasmid

   #213538

   Purpose

   Expresses the CapC-caged opioid peptide in mammalian cells

   Depositor

   Wenjing Wang

   Article

   Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

   Insert

   Signal seuqence-enkephalin-CapC-FLAG-μOR-NNES-SmBit
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-B103 (LM2963)

   Plasmid

   #208960

   Purpose

   A variant CE1 construct with B103 DNA polymerase, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-B103-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A)

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-PolBeta (JO574)

   Plasmid

   #208953

   Purpose

   A variant CE1 construct with human DNA polymerase beta, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-PolBeta-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A)

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CMV-ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-RhoA

   Plasmid

   #165437

   Purpose

   Expresses ShadowY-CBD(H83L/H86L) and Clover(T153M/F223R)-RhoA in mammalian cells as a negative control for FLIM imaging.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-RhoA
   

   Tags

   ShadowY, Clover(T153M/F223R)

   Expression

   Mammalian

   Mutation

   H83L/H86L: CDB(Cdc42 Binding Domain, Pak3, residu…

   Promoter

   CMV

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CMV-ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-Cdc42

   Plasmid

   #165436

   Purpose

   Expresses ShadowY-CBD(H83L/H86L) and Clover(T153M/F223R)-Cdc42 in mammalian cells as a negative control for FLIM imaging.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-Cdc42
   

   Tags

   ShadowY, Clover(T153M/F223R)

   Expression

   Mammalian

   Mutation

   H83L/H86L: CDB(Cdc42 Binding Domain, Pak3, residu…

   Promoter

   CMV

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-SEMPER_ACC-msfBFP[r5M]_ACC-msfGFP[r5M]_ACC-emiRFP670_IRES-mCherry

   Plasmid

   #221081

   Purpose

   SEMPER plasmid for expression of msfGFP[r5M] and msfBFO[r5M] and emiRFP670 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

   Depositor

   Mikhail Shapiro

   Article

   Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

   Insert

   ACC-msfBFP[r5M]_ACC-msfGFP[r5M]_ACC-emiRFP670_IRES-mCherry
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. R620-M14-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 B.1.351

   Plasmid

   #170198

   Purpose

   Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.351 variant)

   Depositor

   Dominic Esposito

   Article

   Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

   Insert

   SARS-CoV-2 S (spike) B.1.351 (S SARS-CoV-2)
   

   Tags

   His6

   Expression

   Mammalian

   Mutation

   L18F, D80A, D215G, Δ(242-244), R246I, K417N, E484…

   Promoter

   CMV51

   Available Since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEF1a_BbsI-gRNA scaffold-EC73-5'-EcoRI-EC73-3'-HDV_pA_pCMV_EGFP-L202S-P2A-mCherry_pA

   Plasmid

   #176461

   Purpose

   Vector for constructing retron Ec73ncRNA-gRNA chimeric RNA (Ec73 rgRNA), inserting spacer sequence by BbsI digestion and inserting donor sequence by EcoRI digestion. EGFP(L202S)-P2A-mCherry driven by CMV

   Depositor

   Hui Yang

   Article

   Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

   Insert

   BbsI-gRNA scaffold-Ec73ncRNA-EcoRI-HDV
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. R620-M15-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 D614G

   Plasmid

   #170201

   Purpose

   Mammalian expression of SARS-CoV-2 soluble spike trimer protein (D614G mutant)

   Depositor

   Dominic Esposito

   Article

   Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

   Insert

   SARS-CoV-2 S (spike) D614G (S SARS-CoV-2)
   

   Tags

   His6

   Expression

   Mammalian

   Mutation

   D614G

   Promoter

   CMV51

   Available Since

   Feb. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEX-CMV-SP-YFP-STM1(24-685) EF hand 3 mut (A79K, N80D, D82G)

    Plasmid

    #18865

    Depositor

    Tobias Meyer

    Article

    Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

    Insert

    Stromal interaction molecule 1 (STIM1 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    3 mutations (A79K, N80D, D82G) in the EF hand of …

    Available Since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-T7Sequenase (JO584)

    Plasmid

    #208955

    Purpose

    A variant CE1 construct with T7 Sequenase DNA polymerase, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-Sequenase-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HUHe variant click editor (CE1) - pCMV-T7-TGMV-nCas9-EcKlenow (JO659)

    Plasmid

    #208951

    Purpose

    A variant CE1 construct with TGMV HUH enconuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    TGMV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. HUHe variant click editor (CE1) - pCMV-T7-FBYNV-nCas9-EcKlenow (JO610)

    Plasmid

    #208950

    Purpose

    A variant CE1 construct with FBYNV HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    FBYNV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HUHe variant click editor (CE1) - pCMV-T7-RepBM-nCas9-EcKlenow (JO606)

    Plasmid

    #208949

    Purpose

    A variant CE1 construct with RepBM HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    RepBm-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HUHe variant click editor (CE1) - pCMV-T7-TraI-nCas9-EcKlenow (JO604)

    Plasmid

    #208948

    Purpose

    A variant CE1 construct with TraI HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    TraI-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CMVTO-p450-hcvs-B2AD2-26Sfur-SEAP-26sFur-B2AD-tevs-KKMP

    Plasmid

    #179644

    Purpose

    AND gate (TEVP/HCVP) SEAP RELEASE construct

    Depositor

    Xiaojing Gao

    Article

    Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

    Insert

    SEAP RELEASE
    

    Expression

    Mammalian

    Available Since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-T7-NLS(SV40)-anCas(BCA)-BPNLS(SV40)-P2A-EGFP (LTH1384)

    Plasmid

    #185488

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(BCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

    Depositor

    Benjamin Kleinstiver, Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage NLS-anCas(BCA)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-P2A-eGFP and NLS

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEX-CMV-SP-YFP-STM2(15-746AA)-EF hand 3 mut (K83A,D84N,G86D)

    Plasmid

    #20174

    Depositor

    Tobias Meyer

    Article

    Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

    Insert

    pEX-CMV-SP-YFP-STM2(15-746AA)-EF hand 3 mut (K83A,D84N,G86D) (STIM2 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    3 mutations (K83A,D84N,G86D) in the EF hand of ST…

    Available Since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. R619-M67-303: CMV51p> SARS-CoV-2 S-RBD(319-541)-His6

    Plasmid

    #166018

    Purpose

    mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD)

    Depositor

    Dominic Esposito

    Article

    Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

    Insert

    SARS-CoV-2 RBD (S )
    

    Tags

    His6

    Expression

    Mammalian

    Promoter

    CMV51

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-T7-NLS(SV40)-anCas(PDCA)-BPNLS(SV40)-P2A-EGFP (LTH1430)

    Plasmid

    #185485

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(PDCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

    Depositor

    Benjamin Kleinstiver, Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage NLS-anCas(PDCA)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-P2A-eGFP and NLS

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge