We narrowed to 43,196 results for: Sis
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Plasmid#197859PurposeExpresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteriaDepositorInsertgRNA scaffold - Tc resistance cassette - incP oriT
UseCRISPR and Synthetic BiologyPromoterPEM7Available SinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHyg-AID*
Plasmid#99515PurposeC-terminal AID* degron cassette with HygR marker for S. cerevisiaeDepositorInsertIAA17(71-114) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteMutationinternal inversion of nucleotides 265-312 of the …Available SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-SF3B1-K700E-reporter
Plasmid#200318PurposeA fluorescent reporter for SF3B1 K700E mutation. Co-expresses a blasticidin resistance gene for selection. Includes LoxP sites for Cre-mediated deletion of reporter cassette.DepositorInsertBSD-2A-EGFPi
UseLentiviralExpressionMammalianPromoterEF1-aAvailable SinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD miniSOG
Plasmid#91774PurposeBacterial expression of a fluorescent protein, mini Singlet Oxygen Generator (miniSOG)DepositorInsertmini Singlet Oxygen Generator
TagsHexa-Histidine tagExpressionBacterialPromoteraraBADAvailable SinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pS848∙CsR
Plasmid#199240PurposeExpresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteriaDepositorInsertgRNA scaffold - Gm resistance cassette - incP oriT
UseCRISPR and Synthetic BiologyPromoterPEM7Available SinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMBEC6
Plasmid#187598PurposeBase editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Gentamycin resistanceDepositorInsertnCas9-BEC-UGI; cas6f, gfp
ExpressionBacterialPromotertrcAvailable SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
lenti-SAM-hygro
Plasmid#138955PurposeLentiviral vector for co-expressing spCas9, dRNA-MS2, p65 and HSF1 fused to MS2 binding protein, and hygromycin resistance geneDepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterU6 AND EF1aAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
CRY2(L348F)-CreN
Plasmid#75368PurposeExpresses mCherry tag, then IRES, then Arabidopsis CRY2 (with L348F mutation) fused to N-terminal fragment of Cre DNA recombinaseDepositorInsertCRY2 (CRY2 Mustard Weed)
TagsCre recombinase, residues 19-104ExpressionMammalianMutationL348FPromoterCMVAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRelA*
Plasmid#175590PurposeTet inducible, KAN Resistant, fluor labelled (YFP) synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.DepositorInsertCatalytic domain of E.coli relA gene.
TagsFused with a flexible GS linker to mVenusExpressionBacterialMutationOnly the catalytic domain of the enzyme is used (…Available SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
gRNA NIA TLS1
Plasmid#196980PurposeExpresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS1 mobility signal that confers graft mobility.DepositorInsertAtNIA1 gRNAs fused to TLS1 tags
ExpressionPlantPromoterpU6-26, pU6-29Available SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKan-PCUP1-AID*(N)-9myc
Plasmid#99526PurposeN-terminal AID*-9myc degron cassette with CUP1 promoter and KanR marker for S. cerevisiaeDepositorInsertIAA17(71-116) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteTags9xMycPromoterCUP1Available SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)
Plasmid#71719PurposeExpressing a GFP-tagged degron in the soma of C. elegansDepositorInsertauxin-responsive protein IAA17 (AXR3 Mustard Weed)
TagsEmGFPExpressionWormMutationminimal degron sequence (71-114aa) with start cod…Promotereef-1A.1 (eft-3)Available SinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
NPY-td-Orange2
Plasmid#83497PurposeExpression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.DepositorAvailable SinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgSlc17a7
Plasmid#124846PurposeMutagenesis of Slc17a7DepositorInsertSlc17a7 (Slc17a7 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCold I-Hero45
Plasmid#187931PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero45DepositorAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
T/Brachyury-eGFP Rex Neo
Plasmid#21222DepositorInsertBrachyury promoter
UseLentiviralTagseGFPAvailable SinceJuly 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Htr5b(3.7)-tTA-sv40
Plasmid#184412PurposeAAV expression of tTA from mouse 5-hydroxytryptamine (serotonin) receptor 5B (Htr5b) gene promoter.DepositorInsertMouse Htr5b gene promoter-tTA
UseAAVTagstTAExpressionMammalianPromoterMouse Htr5b geneAvailable SinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIRES-Puro-MGMT
Plasmid#187654PurposeExpresses hMGMT together with the puromycin resistance gene via an IRES.DepositorAvailable SinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOTTC840 - pAAV 5xATF6 secNLuc
Plasmid#82497PurposeLongitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivoDepositorInsertsecNLuc
UseAAVExpressionMammalianPromoter5x(ATF6 binding site) c-Fos minimal promoterAvailable SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GFP DNAJB1
Plasmid#19495DepositorAvailable SinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only