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UKNP4.3.2

Plasmid

#98270

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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UKNP1.2.2

Plasmid

#97357

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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J23100_GB

Plasmid

#65983

Purpose

MoClo Basic Part: Constitutive promoter - Anderson series - high strength [G:J23100:B]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Constitutive promoter

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-Paxillin-22

Plasmid

#58024

Purpose

Localization: Focal Adhesions, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Paxillin

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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Dendra2-Zyxin-6

Plasmid

#57750

Purpose

Localization: Focal Adhesions, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Zyxin (ZYX Human)

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mOrange2-Paxillin-22

Plasmid

#57970

Purpose

Localization: Focal Adhesions, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Paxillin

Tags

mOrange2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only
StrepII-tag

Plasmid

#55181

Purpose

Streptactin-tag

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

StrepII tag

Use

Synthetic Biology; Bacillus biobrick box

Available Since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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J23101

Plasmid

#55176

Purpose

Very weak constitutive promoter

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

J23101 promoter

Use

Synthetic Biology; Bacillus biobrick box

Promoter

J23101

Available Since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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ACOT11A

Plasmid

#42424

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

ACOT11A (ACOT11 Human)

Tags

His6-TEV

Expression

Bacterial

Mutation

See Comments

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pDONR223-SNX16

Plasmid

#23617

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

SNX16 (SNX16 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-HIPK4

Plasmid

#23760

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

HIPK4 (HIPK4 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
XE11 Xwnt 8b

Plasmid

#16862

Depositor

Randall Moon

Article

Cui et al Development. 1995 Jul. 121(7):2177-86.

Insert

Xwnt-8b (wnt8b Frog)

Use

Xenopus expression

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
pUC18T-mini-Tn7T-hph-dCas9

Plasmid

#218371

Purpose

Expression of dCas9 for CRISPR-interference

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Insert

dCas9

Use

CRISPR

Expression

Bacterial

Promoter

PtetA

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pSfinx-mYFP

Plasmid

#216206

Purpose

A simplified disease resistance assay using YFP-expressing Potato Virus X in N. benthamiana

Depositor

Takaki Maekawa

Article

Hasegawa et al Plant Physiol. 2024 Jul 8:kiae368. doi:

Insert

monomeric yellow fluorescent protein

Expression

Plant

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-C1-GOLGA4-GRIP

Plasmid

#220068

Purpose

overexpreeses the TGN protein GRIP with a mCherry fluorophore

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 22;7:2

Insert

GOLGA4-GRIP (GOLGA4 Human)

Expression

Mammalian

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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FZD8

Plasmid

#155618

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

FZD8 (FZD8 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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PH-PLCd1-mNGx3

Plasmid

#202753

Purpose

expresses fluorescent protein-tagged lipid biosensor

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

PLCD1v2(1-170): mNG2:mNG2:mNG2 (PLCD1 Human)

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_CoA-Snifit(G41)

Plasmid

#197942

Purpose

Expression of CoA-Snifit in mammalian cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Expression

Mammalian

Mutation

PanK=PanK-D127A,L277W,F252Y

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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p15A-OptoCre-tetA-Ptet-Rtet

Plasmid

#189855

Purpose

Cre-inducible activation of tetA for tetracycline resistance.

Depositor

Mary Dunlop

Article

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Insert

Ptet-loxP-TT-loxP-Rtet-tetA

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

Ptet

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pDEINO5

Plasmid

#179489

Purpose

Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation

Depositor

Bogumil Karas

Article

Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1

Insert

aacC1

Use

Synthetic Biology; Bacterial and yeast cloning

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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