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pCF525-AcrIIA15_gb79_Cand27_lenti

Plasmid

#138313

Purpose

Lenti expression of AcrIIA15

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA15

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCF525-AcrIIA14_gb77_Cand9_lenti

Plasmid

#138311

Purpose

Lenti expression of AcrIIA14

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA14

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pBLO 9.6_bCas9-CP1010

Plasmid

#121498

Purpose

TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Type

Empty backbone

Expression

Bacterial

Promoter

p15A

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBLO 9.15_bCas9-CP1249

Plasmid

#121500

Purpose

TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Type

Empty backbone

Expression

Bacterial

Promoter

p15A

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pBLO 9.16_bCas9-CP1282

Plasmid

#121501

Purpose

TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Type

Empty backbone

Expression

Bacterial

Promoter

p15A

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pShuttle-FEN1mED

Plasmid

#35033

Depositor

Sheila Stewart

Article

Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

Insert

FEN1mWT

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Mutation

PT MUTANT E160D

Promoter

CMV

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
pICH82094

Plasmid

#48074

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pET29b- pchB WT

Plasmid

#70099

Purpose

expresses isochorismate-pyruvate lyase from Pseudomonas aeruginosa

Depositor

Audrey Lamb

Article

Zaitseva et al J Biol Chem. 2006 Nov 3;281(44):33441-9.

Insert

pchB

Expression

Bacterial

Promoter

T7

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only
mEos2-CAF1-C-10

Plasmid

#57351

Purpose

Localization: Chromatin Assembly Factor 1, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

CAF1

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pBLO 36.02_ProCas9TEV

Plasmid

#121624

Purpose

TetR_tetR/tetA promoters_bCas9-CP199-TEVlinker-2xflag_CM_P15A

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Type

Empty backbone

Expression

Bacterial

Promoter

p15A

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pSKB3.TM1777

Plasmid

#11421

Depositor

Sung-Hou Kim

Article

Shin et al Biochemistry. 2003 Nov 25. 42(46):13429-

Insert

NusA (nusA T. maritima)

Tags

6x His and TEV protease site

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
hIRmut-GFP

Plasmid

#22397

Depositor

Joseph Bass

Article

Ramos et al Proc Natl Acad Sci U S A. 2007 Jun 19. 1

Insert

IRmut

Tags

GFP

Expression

Mammalian

Mutation

changed Leu 62 to Proline

Available Since

Nov. 29, 2011

Availability

Academic Institutions and Nonprofits only
pHBFD1

Plasmid

#53565

Purpose

Produces an active HBFD (carotenoid 4-hydroxy-β-ring 4-dehydrogenase) enzyme in E. coli.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

Insert

hbfd1 cDNA

Expression

Bacterial

Promoter

lac

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pJK_proJK1_LGK

Plasmid

#81160

Purpose

Expresses levoglucosan kinase with promoter proJK1

Depositor

Timothy Whitehead

Article

Klesmith et al ACS Synth Biol. 2015 Sep 14.

Insert

Levoglucosan kinase wild-type

Tags

His6x

Expression

Bacterial

Promoter

proJK1

Available Since

Sept. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221-Phelo

Plasmid

#48995

Purpose

Gateway adapted transfer of Phleomycin resistance gene

Depositor

Rick Tarleton

Article

Xu et al BMC Microbiol. 2009 May 11;9:90. doi: 10

Insert

Sh ble

Use

Gateway attb1 & attb2

Available Since

April 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pET28CLY8

Plasmid

#21767

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 8 SGGSGG repeats

Tags

His

Expression

Bacterial

Available Since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pTHSSe_32

Plasmid

#109236

Purpose

PUPsp2 GFP reporter

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PUPsp2

Available Since

July 23, 2018

Availability

Academic Institutions and Nonprofits only

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pET28b-TM1570

Plasmid

#30162

Depositor

SeWon Suh

Article

Kim do et al Proteins. 2009 Jan . 74(1):245-9.

Insert

TM1570 (TM1570 Thermotoga maritima)

Tags

His

Expression

Bacterial

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
mEos2-C-Src-7

Plasmid

#57363

Purpose

Localization: Membrane/Vesicles, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

C-Src

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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MSCV-PM mir shHoxB9 b

Plasmid

#27020

Depositor

Joan Massague

Article

Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

Insert

mir HoxB9 3b

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only