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GB1-His-TET1-878-911(pL91)

Plasmid

#124390

Purpose

E.Coli produced TET1 SID (TET1-878-911) purified by FPLC nickel column

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

GB1-His-TET1-878-911 wildtype (TET1 Human)

Tags

GB1-Histidine

Expression

Bacterial

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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Kv7.4 AB (325-557, Δ 368-523)/pET28HMT

Plasmid

#111052

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Tags

His Tag and MBP tag

Expression

Bacterial

Mutation

Kv7.4 AB (325-557, Δ 368-523)

Promoter

T7

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMEL11

Plasmid

#107917

Purpose

amdS based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCAD123_hIrg1_N152K,M154I_pvp008

Plasmid

#239118

Purpose

hACOD1 with N152K and M154I mutations in E.coli expression vector

Depositor

Konrad Buessow

Article

Chen et al Sci Rep. 2023 Jun 26;13(1):10360. doi: 1

Insert

cis-aconitate decarboxylase (ACOD1 Human)

Tags

StrepTag and TEV site

Expression

Bacterial

Mutation

Deleted amino acids 1-3 and 462-end. Mutated N152…

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pColdI-SBP-DDX3_helicase_core

Plasmid

#233622

Purpose

Expression of SBP-tagged helicase core region of DDX3X in E.coli

Depositor

Shintaro Iwasaki

Article

Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

Insert

SBP-DDX3X helicase core (EIF4A1 Human)

Tags

His6-SBP

Expression

Bacterial

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pDEST17-LAP3

Plasmid

#217661

Purpose

For protein purification from Ecoli

Depositor

Karla Feijs

Article

Weixler et al Commun Biol. 2025 Mar 18;8(1):453. doi:

Insert

LAP3 (LAP3 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET-NPR4 (SA binding domain)

Plasmid

#226885

Purpose

Expresses the Salicylic acid binding core (SBC) of NPR4 in E.coli, in an insoluble form

Depositor

Ning Zheng

Article

Wang et al Nature. 2020 Oct;586(7828):311-316. doi:

Insert

The Salicylic acid (SA) binding domain of NPR4 (NPR4 Mustard Weed)

Tags

His tag

Expression

Bacterial

Mutation

NPR4 SA-binding core (residues 373-516), with a s…

Promoter

T7

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6p-1-UFBP1 M2

Plasmid

#204560

Purpose

Expresses GST-tagged UFBP1 M2 in E.coli

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

UFBP1 (DDRGK1 Human)

Expression

Bacterial

Mutation

28-115aa

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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His-ATG3-K62D/K64D

Plasmid

#202786

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-K62D/K64D-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Tags

6xHis, TEV

Expression

Bacterial

Mutation

K62D, K64D

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6p-1-UFBP1 M1

Plasmid

#204559

Purpose

Expresses GST-tagged UFBP1 M1 in E.coli

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

UFBP1 (DDRGK1 Human)

Expression

Bacterial

Mutation

28-314aa

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pET-Duet-6xHis-TEV-Nsp8(76-198)

Plasmid

#201024

Purpose

Bacterial expression of codon optimized SARS-CoV-2 nsp8 residues 76-198, with an N-terminus His tag followed by a TEV cleavage site.

Depositor

Sharon Rozovsky

Article

Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

Insert

non-structural protein 8 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pET-DEST42-dre4

Plasmid

#170440

Purpose

Express drosophila dre4 in E.coli expression system. Generated from pDONR-dre4 by Gateway system. Used for custom antibody purification.

Depositor

Joan Conaway

Article

Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

Insert

dre4 (dre4 Fly)

Tags

6xHis and V5

Expression

Bacterial

Mutation

Deleted amino acids 1-20

Promoter

T7

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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pSUMO R106L-CPTP

Plasmid

#170737

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

Insert

R106L-CPTP (CPTP Human)

Tags

SUMO

Expression

Bacterial

Mutation

R106L

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pSUMO K60A-CPTP

Plasmid

#170736

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

Insert

K60A-CPTP (CPTP Human)

Tags

SUMO

Expression

Bacterial

Mutation

K60A

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pETM33_Nsp3c_SUD-C

Plasmid

#156471

Purpose

Bacterial expression of Sars-CoV2 Nsp3c_SUD-C protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3c_SUD-C (ORF1ab Synthetic)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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GB1-His-TET1-878-911-L900A(pL91)

Plasmid

#124395

Purpose

E.Coli produced TET1-SID mutants (L900A) purified by FPLC nickel column

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

GB1-His-TET1-878-911-L900A (TET1 Human)

Tags

GB1-Histidine

Expression

Bacterial

Mutation

L900A

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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GB1-His-TET1-878-911-T898E(pL91)

Plasmid

#124394

Purpose

E.Coli produced TET1-SID mutants (T898E) purified by FPLC nickel column

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

GB1-His-TET1-878-911-T898E (TET1 Human)

Tags

GB1-Histidine

Expression

Bacterial

Mutation

T898E

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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GB1-His-TET1-878-911-T898A(pL91)

Plasmid

#124393

Purpose

E.Coli produced TET1-SID mutants (T898A) purified by FPLC nickel column

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

GB1-His-TET1-878-911-T898A (TET1 Human)

Tags

GB1-Histidine

Expression

Bacterial

Mutation

T898A

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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GB1-His-TET1-878-911-L897A(pL91)

Plasmid

#124392

Purpose

E.Coli produced TET1-SID mutants (L897A) purified by FPLC nickel column

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

GB1-His-TET1-878-911-L897A (TET1 Human)

Tags

GB1-Histidine

Expression

Bacterial

Mutation

L897A

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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GB1-His-TET1-878-911-I894A(pL91)

Plasmid

#124391

Purpose

E.Coli produced TET1-SID mutants (I894A) purified by FPLC nickel column

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

GB1-His-TET1-878-911-I894A (TET1 Human)

Tags

GB1-Histidine

Expression

Bacterial

Mutation

I894A

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST15.1-hRPN13 (285-407) (Q337A/Q338A)

Plasmid

#61935

Purpose

Expression of hRPN13 (285-407) (Q337A/Q338A) in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

hRPN13 (285-407) (ADRM1 Human)

Tags

GST

Expression

Bacterial

Mutation

Construct starts at residue 285, residues Q337 an…

Promoter

T7

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
Kv7.4 AD (325-645, C643A, Δ 368-492)/pET28HMT

Plasmid

#111072

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Tags

His Tag and MBP tag

Expression

Bacterial

Mutation

Kv7.4 AD (325-645, C643A, Δ 368-492)

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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Kv7.5 AB (361-545, Δ395-511)/pET28HMT

Plasmid

#111267

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 5 isoform 1 [Homo sapiens] (KCNQ5 Human)

Tags

His Tag and MBP Tag

Expression

Bacterial

Mutation

Kv7.5 AB (361-545, Δ395-511)

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pDEST17-ALDH1A3

Plasmid

#189750

Purpose

The construct was used to express recombinant ALDH1A3 in E.Coli. The purified protein was used for activity assay.

Depositor

Robert Sobol

Article

Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

Insert

ALDH1A3 (ALDH1A3 Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST17-ALDH1A1

Plasmid

#189749

Purpose

The construct was used to express recombinant ALDH1A1 in E.Coli. The purified protein was used for activity assay.

Depositor

Robert Sobol

Article

Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

Insert

ALDH1A1 (ALDH1A1 Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-23Q

Plasmid

#84349

Purpose

Expression of the human Huntingtin Exon1 protein containing 23Q in E.coli

Depositor

Hilal Lashuel

Article

Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

Insert

Huntingtin Exon 1 (HTT Human)

Tags

N-terminal Ssp intein (His-tagged)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 24, 2018

Availability

Academic Institutions and Nonprofits only

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TRPML1-mCherry

Plasmid

#135189

Purpose

Expresses TRPML1 with C-terminus mCherry tag in mammalian cells.

Depositor

Antony Galione

Article

Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

Insert

TRPML1 (Mcoln1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1 ElonginB, ElonginC

Plasmid

#230999

Purpose

Dual expression vector containing mouse ElonginB and ElonginC for E.coli protein purification

Depositor

Michael Rape

Article

Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

Inserts

Elongin B (Elob Mouse)
Elongin C (Eloc Mouse)

Expression

Bacterial

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pETM33_Nsp16

Plasmid

#156482

Purpose

Bacterial expression of Sars-CoV2 Nsp16 protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp16 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pMG024 MBP-GSK3β-HA-His, GST_λPPase

Plasmid

#196182

Purpose

Co-expresses MBP-GSK3β-HA-His (human GSK3β as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β-HA-His

Depositor

Jesse Zalatan

Article

Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755

Inserts

GSK3B (GSK3B Human)
λPPase

Tags

GST, HA, His6, and MBP

Expression

Bacterial

Promoter

Tac

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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