We narrowed to 23,628 results for: CRISPR

1. pX330-BbsI-PITCh

   Plasmid

   #127875

   Purpose

   For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly

   Depositor

   Peter Kaiser

   Article

   Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRIS-PITChv2-FBL

   Plasmid

   #63672

   Purpose

   PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

   Insert

   EGFP-2A-PuroR
   

   Use

   CRISPR

   Promoter

   Promoterless

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. p2CT-His-MBP-Hhe_Cas13a_WT

   Plasmid

   #91871

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   HheCas13a (HheC2c2) WT
   

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Available Since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEL680

   Plasmid

   #207513

   Purpose

   GmM4-SpRY CBE-STU

   Depositor

   Yong Zhang

   Article

   Chen et al Hortic Res. 2024 Jun 7;11(8):uhae160. do

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Promoter

   GmM4

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-C-dTAG-Blast

   Plasmid

   #207716

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. qTAG-N-Blast-dTAG

   Plasmid

   #207683

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-dTAG cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pMM178

   Plasmid

   #61270

   Purpose

   Also referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains a pBBR1 origin and chloramphenicol resistance.

   Depositor

   Timothy Lu

   Article

   Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

   Insert

   Cas9, tracrRNA, crRNA
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   pLtetO, native, native (respectively)

   Available Since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-SCP1-dSa-VPR mini.

   Plasmid

   #99685

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Use

   AAV

   Tags

   VPR mini

   Mutation

   dead Cas9

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMBP-FnCas12a

   Plasmid

   #113432

   Purpose

   Expresses 10xHis-MBP fused to FnCas12a

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   FnCas12a
   

   Tags

   MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #113341

    Purpose

    Expresses VP64-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

    Depositor

    Gavin Wright

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    VP64-dCas9-VP64-T2A-MS2-p65-HSF1
    

    Use

    Piggybac

    Expression

    Mammalian

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. qTAG-N-Blast-mStayGold

    Plasmid

    #227251

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-mStayGold cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. qTAG-C-mStayGold-Blast

    Plasmid

    #227254

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLBH531_MBP-Cas14a1 expression

    Plasmid

    #112500

    Purpose

    Expresses MBP fused to Cas14a1

    Depositor

    Jennifer Doudna

    Article

    Harrington et al Science. 2018 Oct 18. pii: science.aav42

    Insert

    Cas14a1
    

    Use

    CRISPR

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. qTAG-N-Puro-mScarlet

    Plasmid

    #207689

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mScarlet cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. qTAG-C-mStayGold-Puro

    Plasmid

    #227253

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJAT7

    Plasmid

    #204292

    Purpose

    For inserting a phiC31 attP site. HDR integrant is marked with removable 3XP3-DsRed cassette, which is expressed in eyes. DsRed difficult to see in wild type eyes if using LeicaM165 FC microscope.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    3XP3-DsRed
    

    Use

    CRISPR

    Promoter

    3XP3

    Available Since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. qTAG-N-Puro-mStayGold

    Plasmid

    #227250

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mStayGold cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSF278

    Plasmid

    #167673

    Purpose

    Intermediate vector for three gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSGAb-km

    Plasmid

    #121999

    Purpose

    A sgRNA expression plasmid for genome editing in Acinetobacter baumannii

    Depositor

    Quanjiang Ji

    Article

    Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

    Insert

    none
    

    Use

    CRISPR

    Available Since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. qTAG-C-3xFLAG-Puro

    Plasmid

    #207728

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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