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pcDNA5/FRT/TO APEX2-GFP

Plasmid

#129640

Purpose

Express APEX2-GFP fusion in mammalian cells

Depositor

Nicholas Ingolia

Article

Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

Insert

APEX2-GFP

Expression

Mammalian

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pinducer 20 DN-KASHΔPPPL

Plasmid

#129280

Purpose

Tet-ON inducible lentivirus expressing mcherry-labeled DN KASHΔPPPL

Depositor

Daniel Conway

Article

Zhang et al Curr Biol. 2019 Aug 2. pii: S0960-9822(1

Insert

SYNE1 (SYNE1 Human)

Use

Lentiviral

Tags

mcherry

Mutation

the last 4 amino acids (PPPL) of the KASH domain …

Promoter

CMV

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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mTF-CRISPRai

Pooled Library

#122131

Purpose

Increases transcriptional repression of genes encoding transcription factors in combination with dCas9 repression systems, such as the dCas9-KRAB system.

Depositor

Stanley Qi

Article

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available Since

April 25, 2019

Availability

Academic Institutions and Nonprofits only
pGTag-gal4-VP16-B-actin

Plasmid

#117817

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

gal4/VP16

Use

Synthetic Biology

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-GCaMP6m-Xn

Plasmid

#118976

Purpose

Nuclear localized GCaMP-X sensor for monitoring calcium signal in nucleus

Depositor

Xiaodong Liu

Article

Yang et al Nat Commun. 2018 Apr 17;9(1):1504. doi:

Insert

GCaMP6m-XN

Expression

Mammalian

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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2Flag-pDEST-N

Plasmid

#118371

Purpose

Gateway compatible mammalian expression vector with N-terminal 2Flag to tag proteins of interest.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

2Flag

Expression

Mammalian

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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CMV Flag ATRwt

Plasmid

#41909

Depositor

Stephen Elledge

Article

Cortez et al Science. 2001 Nov 23;294(5547):1713-6.

Insert

ATR ATR serine/threonine kinase (ATR Human)

Tags

FLAG

Expression

Mammalian

Promoter

SRa

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-152

Plasmid

#118566

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sGFP2

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pLX311-Cas9

Plasmid

#118018

Purpose

Constitutive expression of Cas9

Depositor

William Hahn, David Root

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

Cas9

Use

Lentiviral

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pUS2-SH231

Plasmid

#115150

Purpose

Cas9-GFP, dual gRNA expression vector for targeted integration of repair templates into safe harbor 231 locus in chromosome 4

Depositor

Raymond Monnat, Michael Phelps

Article

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

Dual SH231 gRNAs

Use

CRISPR

Tags

3xFLAG-SV40NLS, GFP, and Nucleoplasmin NLS

Expression

Mammalian

Promoter

U6, CBh

Available Since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pDe-CAS9

Plasmid

#61433

Purpose

Binary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequence

Depositor

Holger Puchta

Article

Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

Insert

Cas9

Use

CRISPR

Expression

Plant

Promoter

PcUBI4-2

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pX458-DsRed2

Plasmid

#112219

Purpose

Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2

Depositor

Yinming Liang

Article

Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

Insert

DsRed2

Use

CRISPR

Expression

Mammalian

Mutation

DsRed2 contains a silent nucleotide substitution …

Available Since

July 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-shNT

Plasmid

#109012

Purpose

short-hairpin knockdown of targeted gene

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

Non-targeting shRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6 promoter

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-NES-PhoCl-mCherry

Plasmid

#87690

Purpose

Express NES-PhoCl-mCherry in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

NES-PhoCl-mCherry

Tags

nuclear export signal

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pUAS-mCherry-NLS

Plasmid

#87695

Purpose

Gal4VP16 driven expression of mCherry-NLS in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

mCherry-NLS

Tags

nuclear localization signal

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGmCherry-gRNA

Plasmid

#87110

Purpose

gRNA cloning vector with CAGmCherry

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pPEPY-PF6-lacI

Plasmid

#85589

Purpose

Work as template for amplification of Streptococcus pneumoniae adapted lacI gene, which is flanked by up- and downstream sequence for integration in prsA locus in S. pneumoniae

Depositor

Jan-Willem Veening

Article

Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

Insert

lacI

Expression

Bacterial

Promoter

PF6

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pRN12

Plasmid

#84456

Purpose

shuttle plasmid for sarA P1-mAmetrine expression

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

mAmetrine

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pAG303-Gal-GR100

Plasmid

#84908

Purpose

induce expression of dipeptide repeat GR in yeast

Depositor

Aaron Gitler

Article

Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

Insert

GR100 (C9orf72 Synthetic)

Expression

Yeast

Promoter

GAL1

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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Sumo3 PolQM1

Plasmid

#78462

Purpose

Inducible expression in E. coli

Depositor

Sylvie Doublie, Susan Wallace

Article

Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

Insert

Polymerase theta (POLQ Human)

Tags

His and SUMO3

Expression

Bacterial

Mutation

delta 1-1791; Q2513R (please see depositor commen…

Promoter

T7

Available Since

July 28, 2016

Availability

Academic Institutions and Nonprofits only