We narrowed to 19,051 results for: Tec;

1. pTS1024-Tier1-PRPBSA-YPet-p2A-PuroR

   Plasmid

   #169499

   Purpose

   Tier-1 vector encoding PRPBSA-driven YPet in combination with a marker for puromycine selection (PRPBSA-YPet-p2A-PuroR-pA)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PRPBSA-driven YPet-P2aA-PuroR
   

   Expression

   Mammalian

   Promoter

   PRBSA

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTS1047-Tier1-PhCMV-YPet-p2A-PuroR

   Plasmid

   #169498

   Purpose

   Tier-1 vector encoding PhCMV-driven YPet in combination with a marker for puromycin selection (PhCMV-YPet-p2A-PuroR-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven YPet-P2aA-PuroR
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. 1056H

   Plasmid

   #183135

   Purpose

   Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.

   Depositor

   Omar Akbari

   Article

   Kandul et al GEN Biotechnology (2022) 1:4, 372-385

   Insert

   U6.3-gRNAs[sxl, bTub]
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   June 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. 1056J

   Plasmid

   #183136

   Purpose

   Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.

   Depositor

   Omar Akbari

   Article

   Kandul et al GEN Biotechnology (2022) 1:4, 372-385

   Insert

   U6.3-gRNAs[sxl, bTub]
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   June 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. 1056K

   Plasmid

   #183137

   Purpose

   Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.

   Depositor

   Omar Akbari

   Article

   Kandul et al GEN Biotechnology (2022) 1:4, 372-385

   Insert

   U6.3-gRNAs[sxl, bTub]
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   June 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX459-sg-CMV152

   Plasmid

   #179916

   Purpose

   All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 152.

   Depositor

   Indeever Madireddy, Johan Sosa

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   CMV promoter sgRNA
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX459-sg-tdTomato166/892

   Plasmid

   #179919

   Purpose

   Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 166 and 892.

   Depositor

   Indeever Madireddy, Johan Sosa

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   tdTomato sgRNA
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX459-sg-CMV51

   Plasmid

   #179915

   Purpose

   All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 51.

   Depositor

   Indeever Madireddy, Johan Sosa

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   CMV promoter sgRNA
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX459-sg-CMV162

   Plasmid

   #179917

   Purpose

   All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 162.

   Depositor

   Indeever Madireddy, Johan Sosa

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   CMV promoter sgRNA
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX459-sg-mGreenLantern332

    Plasmid

    #179914

    Purpose

    All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern fluorescent protein site 332.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    mGreenLantern sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. 1137B=Hsp70Bb-Cas9

    Plasmid

    #153284

    Purpose

    The attB plasmid harboring the Hsp70Bb-Cas9-T2A-eGFP-p10 and a mini-white marker.

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

    Insert

    pHsp70Bb-SpCas9-T2A-eGFP-p10 (cas9 Synthetic)
    

    Use

    CRISPR

    Tags

    eGFP

    Expression

    Insect

    Promoter

    Dmel Hsp70Bb

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC19-SpRYgest_targetplasmid (KAC833)

    Plasmid

    #181748

    Purpose

    pUC19 derivative plasmid used as a substrate for SpRYgest, restriction enzyme, and TtAgo digests. Encodes an SpCas9 EMX1-site1-NGG-PAM target site between NheI and HindIII restriction sites.

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Type

    Empty backbone

    Use

    Substrate plasmid

    Available Since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pG.Lantern-INS-SM50-GFP

    Plasmid

    #135594

    Purpose

    Propogation of Insulator-SM50 Promoter-GFP DNA for microinjection to visualize Insulator-GFP promoter activity in S. purpuratus

    Depositor

    Koji Akasaka, Mamiko Yajima

    Article

    Yajima et al Dev Genes Evol. 2007 Apr;217(4):331-6. d

    Insert

    INS-SM50 promoter (SM50 )
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    Green Lantern GFP

    Promoter

    CMV

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBS-tRNA-Gly

    Plasmid

    #174317

    Purpose

    For in vitro transcription of mouse tRNAs

    Depositor

    Bruce Beutler

    Article

    Yue et al Science. 2021 May 14;372(6543). pii: 372

    Insert

    tRNA-gly
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. PdnaK-IR3 GFP pZa

    Plasmid

    #170084

    Purpose

    GFP expression under the control of E. coli dnaK promoter engineered with IR3 HAIR motif from M. tuberculosis

    Depositor

    Urartu Seker

    Article

    Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

    Insert

    Green fluorescent protein
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PdnaK-IR3

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. PdnaK-IR2 GFP pZa

    Plasmid

    #170081

    Purpose

    GFP expression under the control of E. coli dnaK promoter engineered with IR2 HAIR motif from M. tuberculosis

    Depositor

    Urartu Seker

    Article

    Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

    Insert

    Green fluorescent protein
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PdnaK-IR2

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a(+)-HisTim12_CtoA

    Plasmid

    #170281

    Purpose

    Expresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

    Depositor

    Paul Schanda

    Article

    Weinhaupl et al Structure. 2021 May 3. pii: S0969-2126(2

    Insert

    yeast Tim12 (TIM12 Budding Yeast)
    

    Tags

    TEV-cleavable N-terminal His tag (on backbone)

    Expression

    Bacterial

    Mutation

    mutated two non-conserved Cys to Ala

    Available Since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. KG#920

    Plasmid

    #110937

    Purpose

    Expresses INS-22-Emerald in the cholinergic motor neuron DA9 for imaging Dense Core Vesicles in a single neuron in a living animal

    Depositor

    Kenneth Miller

    Article

    Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:

    Inserts

    mig-13 promoter
    INS-22-Emerald
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    

    Expression

    Bacterial

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUE1-TSP-ORF68 R14A/K310A

    Plasmid

    #162655

    Purpose

    Expresses ORF68 R14A/K310A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Mutation

    Amino acids R14 and K310 mutated to alanine

    Promoter

    CMV

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLJM1-zeo-2xStrep-ORF68 C52A

    Plasmid

    #162660

    Purpose

    Lentiviral vector for stable expression of N-terminal 2xStrep-tagged ORF68 C52A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Lentiviral

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acid C52 mutated to alanine

    Promoter

    CMV

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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