Addgene

We narrowed to 9,331 results for: try

Showing: 6741 - 6760 of 9331 results

Results per page:

Sort results by:

pLV-EF1A-LivePAR(Y107A)-Hygro

Plasmid

#176073

Purpose

EGFP fused to the C-terminus of a WWE domain containing the mutation Tyr107Ala & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

LivePAR (Y107A)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Point mutation to convert Try107 to Ala

Promoter

EF1A

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
hFLT1

Plasmid

#83435

Purpose

human VEGFR1 with HA and FLAG tag

Depositor

Christie Thomas

Article

Raikwar et al Exp Cell Res. 2013 Oct 15;319(17):2578-8

Insert

human VEGFR1 (FLT1 Human)

Use

Tags

Flag and Myc

Expression

Mammalian

Mutation

HA-tag was inserted 30 AA downstream of the FLT1 …

Promoter

CMV

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
peGFP C3-SPG20 8xV

Plasmid

#218579

Purpose

Expresses GFP-tagged human SPG20 bulky hydrophobic amino acid mutant in mammalian cells

Depositor

Hemmo Meyer

Article

Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

Insert

SPG20 (SPART Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

changed Tryptophan 419, Leucine 438, Isoleucine 4…

Promoter

CMV

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-ER-EBFP2

Plasmid

#231183

Purpose

Expresses control plasmid for ER-mitochondrial linkers, tagged with EBFP2, in mammalian cells

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

ER-EBFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-10nm-EBFP2

Plasmid

#231184

Purpose

Expresses a synthetic linker, tagged with EBFP2, Stabilizing the ER-mitochondrial distance at 10 nm

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

10nm-EBFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-linker-TlcnC

Plasmid

#114377

Purpose

expresses Flpo recombinase-dependent GtACR2-Kv2.1C-linker-TlcnC, which targets GtACR2 to the somatodendritic compartment. Messier et al found this hybrid construct was the most effective at targeting

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-Kv2.1C-linker-TlcnC (NEWENTRY )

Use

AAV

Tags

EYFP and Kv2.1C-linker-TlcnC

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221_SLC35D3

Plasmid

#132110

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35D3 (SLC35D3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR221_SLC2A3

Plasmid

#132114

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC2A3 (SLC2A3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR221_SLC6A6

Plasmid

#132196

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC6A6 (SLC6A6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 28, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
8xNFAT-ZsG-muhCD4

Plasmid

#162745

Purpose

NFAT-driven ZsGreen-1 reporter gene with mutated human CD4 gene

Depositor

Maki Nakayama

Article

Landry et al Front Endocrinol (Lausanne). 2021 Mar 25

Insert

8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (CD4 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Human CD4 gene with glutamine to tyrosine at posi…

Promoter

Available since

April 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221_SLC6A14

Plasmid

#131865

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC6A14 (SLC6A14 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR221_SLC35F2

Plasmid

#132121

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35F2 (SLC35F2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR221_SLC6A19

Plasmid

#132231

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC6A19 (SLC6A19 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA3.1-ER-mRFP

Plasmid

#231176

Purpose

Expresses control plasmid for ER-mitochondrial linkers, tagged with mRFP, in mammalian cells

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

ER-mRFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221_SLC5A2

Plasmid

#132241

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC5A2 (SLC5A2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR221_SLC19A3

Plasmid

#132045

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC19A3 (SLC19A3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR221_UCP3

Plasmid

#132258

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

UCP3 (UCP3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pENTR-myr-AKT-HA

Plasmid

#31790

Depositor

Xin Chen

Article

Calvisi et al Gastroenterology. 2011 Mar;140(3):1071-8

Insert

Akt1 (Akt1 Mouse)

Use

Entry vector

Tags

HA and Myr

Expression

Mutation

Promoter

Available since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only
pUC19-hAPOE-R-loop

Plasmid

#134901

Purpose

Generation and purification of R-loops for in vitro biochemistry

Depositor

Andrew Deans

Article

Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

Insert

APOE promoter (APOE Human)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221_SLC28A3

Plasmid

#132151

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC28A3 (SLC28A3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge