We narrowed to 1,731 results for: plasmids pcas

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pCAG-Sox2-IRES-Neo

Plasmid

#13462

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

Sox2 (Sox2 Mouse)

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pAAV-pCaMKII-EBFP-tTAn

Plasmid

#172124

Purpose

Expression of EBFP and tTA-InteinN in CaMKII positive cells

Depositor

Chun Xu

Article

Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

Insert

pCaMKII, EBFP, tTAn-InteinN

Use

AAV

Promoter

EF1a

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-dSpCas9-HF1

Plasmid

#92115

Purpose

Expression plasmid for human codon-optimized dead/inactive high-fidelity SpCas9-HF1 (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

dead/inactive SpCas9-HF1 with FLAG tag

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

D10A, N497A, R661A, Q695A, H840A, Q926A

Promoter

Cbh

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCALNL-tdTomato-2A-mSyp::EGFP

Plasmid

#191213

Purpose

Expresses tdTomato protein and mouse Synaptophysin protein fused with EGFP in Cre expressing mammalian cells.

Depositor

Theofanis Karayannis

Article

Gesuita et al STAR Protoc. 2022 Nov 11;3(4):101837. do

Insert

tdTomato-T2A-mSyp::EGFP (Syp Mouse)

Use

Pcagen

Tags

mSyp::EGFP

Expression

Mammalian

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-TadCBEd-SpCas9-P2A-EGFP (BKS327)

Plasmid

#223123

Purpose

pCMV and pT7 Human expression plasmid for TadCBEd-SpCas9 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadCBEd-SpCas9-2xUGI-P2A-EGFP

Use

CRISPR

Tags

2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

Expression

Mammalian

Mutation

TadA-CDd mutations; nSpCas9=D10A

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-SpCas9(KRHWMR)-P2A-EGFP_(RAS3808)

Plasmid

#228251

Purpose

pCMV human expression plasmid for SpCas9 enzyme with KRHWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(KRHWMR)-P2A-EGFP

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(ARGIMR)=D1135K, S1136R, G1218H, E1219W, R1…

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-3xFLAG-NLS-SpCas9-NLS

Plasmid

#51307

Purpose

Cas9 expression plasmid

Depositor

Yonglong Chen

Article

Guo et al Development. 2014 Jan 8.

Insert

3xFLAG-NLS-SpCas9-NLS

Use

CRISPR; Xenopus tropicalis expression

Tags

3x Flag and NLS

Promoter

CMV

Available Since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only

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NFATc2-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75234

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc2 (1/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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NFATc2-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75235

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc2 (2/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCART0016_CROPseq-CAR-Puro_GPC3-BBz

Plasmid

#242985

Purpose

GPC3-BBz CAR

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

GPC3-BBz CAR

Use

Lentiviral

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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AAV pCAG-mRuby3-WPRE

Plasmid

#107744

Purpose

Can be used to express mRuby3. Can also be used to create adeno-associated virus for delivery of the mRuby3 sequence.

Depositor

Rylan Larsen

Article

Zhuang et al Cell Rep. 2021 Oct 12;37(2):109826. doi:

Insert

mRuby3

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-Oct3/4-IRES-Neo

Plasmid

#13461

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

Oct3/4 (Pou5f1 Mouse)

Expression

Mammalian

Mutation

P31S

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pX330-NLC-DHFR-SpCas9

Plasmid

#124523

Purpose

Expresses SpCas9 fused to DHFR domains on both the N- and C- termini and an internal loop in mammalian cells

Depositor

Amit Choudhary

Article

Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

Insert

NLC-DHFR-SpCas9

Use

AAV

Tags

Destabilized domain of E.coli dihydrofolate reduc…

Expression

Mammalian

Promoter

Cbh

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCART0017_CROPseq-CAR-Puro_GD2-BBz

Plasmid

#242984

Purpose

GD2-BBz 14g2a CAR

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

GD2-BBz 14g2a CAR

Use

Lentiviral

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCART0002_pIVTRup(BsmBI)_CBE4max-SpRY

Plasmid

#242540

Purpose

IVT

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

CBE4max-SpRY (SpRY-CBE)

Use

CRISPR

Mutation

D10A

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCART0003_pIVTRup(BsmBI)_ABEmax7.10-SpRY

Plasmid

#242539

Purpose

IVT

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

ABEmax7.10-SpRY (SpRY-ABE)

Use

CRISPR

Mutation

D10A

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCA528 IST1 (322-366)

Plasmid

#193038

Purpose

Bacterial expression plasmid for IST1 (322-366), the minimal construct with full binding to CAPN7 MIT domains.

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

IST1 (IST1 Human)

Tags

6xHis-Sumo

Expression

Bacterial

Mutation

Residues 322-366

Promoter

T7

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG CAPN7 V18D-OSF

Plasmid

#193042

Purpose

Mammalian expression plasmid encoding full-length CAPN7 with V18D mutation that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

CAPN7 (CAPN7 Human)

Tags

OSF (One-Strep-FLAG)

Expression

Mammalian

Mutation

V18D

Promoter

Chicken Beta-actin

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG CAPN7 L61D-OSF

Plasmid

#193043

Purpose

Mammalian expression plasmid encoding full-length CAPN7 with L61D mutation. Contains C-terminal OSF tag (One-Strep-FLAG)

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

CAPN7 (CAPN7 Human)

Tags

OSF (One-Strep-FLAG)

Expression

Mammalian

Mutation

L61D

Promoter

Chicken Beta-actin

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG CAPN7 F98D-OSF

Plasmid

#193044

Purpose

Mammalian expression plasmid encoding full-length CAPN7 with F98D mutation that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

CAPN7 (CAPN7 Human)

Tags

OSF (One-Strep-FLAG)

Expression

Mammalian

Mutation

F98D

Promoter

Chicken Beta-actin

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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