We narrowed to 1,942 results for: 974

1. pDEST-Myc-FBW7 Delta F-Box

   Plasmid

   #197452

   Purpose

   The plasmid expresses F-box deleted version of Myc-tagged FBW7 human isoform 1. Used for mammalian overexpression of FBW7alpha delta-F-Box and detection by western blotting.

   Depositor

   Omar Khan

   Article

   Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

   Insert

   FBW7 (FBXW7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   FBW7 Fbox deleted.

   Promoter

   CMV

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLenti-PGK-EGFP-ALDH1A3-Neo

   Plasmid

   #189744

   Purpose

   The construct was used to express GFP tagged human ALDH1A3 in mammalian cells for the analysis of the subcellular location of this protein.

   Depositor

   Robert Sobol

   Article

   Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

   Insert

   ALDH1A3 (ALDH1A3 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223_DNAJC12_WT

   Plasmid

   #81974

   Purpose

   Gateway Donor vector containing DNAJC12, part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, William Hahn, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   DNAJC12 (DNAJC12 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. iMb-Notch-Mosaic (IR99.40)

   Plasmid

   #99749

   Purpose

   Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different membrane localized fluorescent proteins and Notch signalling genes

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   MbYFP, MbTomato, MbKate2, DN-Rbpj and NICD-PEST
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. 37_pAAV-ProC40-CatCh-GFP-WPRE

   Plasmid

   #125974

   Purpose

   Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

   Depositor

   Botond Roska

   Article

   Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

   Insert

   CatCh-GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   ProC40

   Available since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223_DOK1_WT_V5

   Plasmid

   #82974

   Purpose

   Gateway Donor vector containing DOK1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

   Depositor

   Matthew Meyerson, David Root, Jesse Boehm

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   DOK1 (DOK1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDEST-Myc-FBW7 Delta WD40 (-2 to 7)

   Plasmid

   #197454

   Purpose

   The plasmid expresses delta WD40 deleted version of Myc-tagged FBW7 human isoform 1. WD40 propellars 2 to 7 are deleted. Used for mammalian overexpression and detection by western blotting.

   Depositor

   Omar Khan

   Article

   Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

   Insert

   FBW7 Delta-WD40 propeller (FBXW7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   FBW& dimerization domain deleted.

   Promoter

   CMV

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDEST-Myc-FBW7 WD40 (All deleted)

   Plasmid

   #197455

   Purpose

   The plasmid expresses delta WD40 deleted version of Myc-tagged FBW7 human isoform 1. WD40 All are deleted. Used for mammalian overexpression and detection by western blotting.

   Depositor

   Omar Khan

   Article

   Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

   Insert

   FBW7 (FBXW7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   FBW& dimerization domain deleted.

   Promoter

   CMV

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pD649-HAsp-LRIG1-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156974

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   LRIG1 (LRIG1 Human)
   

   Use

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-T7-ABE8.20m-SpCas9-NRRH-P2A-EGFP (RMD47)

    Plasmid

    #197499

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRRH-BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8.20 mutations in TadA and nSpCas9-NRRH(D10A/I…

    Promoter

    CMV and T7

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGGG-Sr43

    Plasmid

    #186974

    Purpose

    Sr43 is a wheat stem rust resistance gene transferred from tall wheat grass (Thinopyrum ponticum) into bread wheat (Triticum aestivum).

    Depositor

    Brande Wulff

    Article

    Yu et al Nat Genet. 2023 Jun;55(6):921-926. doi:

    Insert

    Sr43
    

    Use

    Tags

    None

    Expression

    Bacterial and Plant

    Mutation

    None

    Promoter

    Natice

    Available since

    April 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. PF3D7_0511400-COMP-blac-flag-his

    Plasmid

    #110974

    Purpose

    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.

    Depositor

    Gavin Wright

    Article

    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi

    Insert

    conserved Plasmodium protein (PF3D7_0511400 Synthetic)
    

    Use

    Tags

    Exogenous signal peptide of mouse origin and rat …

    Expression

    Mammalian

    Mutation

    All NXT/S sites mutated to NXA, where X is any am…

    Promoter

    CMV

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CoV2-Spike-D614G-D1118H

    Plasmid

    #177974

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, D1118H)

    Depositor

    Melanie Ott

    Article

    Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

    Insert

    SARS-CoV-2 Spike protein (D614G, D1118H) (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D614G, D1118H

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. T7-VEE-OKS-iG

    Plasmid

    #58974

    Purpose

    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORF

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Inserts

    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    GLIS (GLIS1 Human)
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pNLF1-N_PWP1:1-501

    Plasmid

    #210974

    Purpose

    Mammalian expression of full-length human PWP1. N-terminal NanoLuc luciferase tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    PWP1:M1-S501 (PWP1 Human)
    

    Use

    Tags

    NanoLuc luciferase

    Expression

    Mammalian

    Mutation

    E165D F448L G449R not annotated variants

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. plp

    Plasmid

    #22310

    Depositor

    David Bartel

    Article

    Drinnenberg et al Science. 2009 Sep 10. ():.

    Type

    Empty backbone

    Use

    Integrating plasmid

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRS403-PGAL1-strongSC_GFP

    Plasmid

    #22316

    Depositor

    David Bartel

    Article

    Drinnenberg et al Science. 2009 Sep 10. ():.

    Insert

    GFP-rad9-GFP
    

    Use

    Integrating plasmid

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pYES2.1-Dcr1

    Plasmid

    #22307

    Depositor

    David Bartel

    Article

    Drinnenberg et al Science. 2009 Sep 10. ():.

    Insert

    DCR1
    

    Use

    2micron

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYES2.1-Ago1

    Plasmid

    #22306

    Depositor

    David Bartel

    Article

    Drinnenberg et al Science. 2009 Sep 10. ():.

    Insert

    AGO1
    

    Use

    2micron

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mEmerald-TPX2-N-10

    Plasmid

    #54285

    Purpose

    Localization: Centrosomes, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    TPX2 (TPX2 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits