We narrowed to 13,934 results for: TIM;

1. pCMV-T7-ABE8.20m-nSpRY-P2A-EGFP (KAC1335)

   Plasmid

   #185917

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   human codon optimized ABE8.20m-nSpRY-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-GST-ATG9 (828-839)-ATG101 (1-198)

   Plasmid

   #188073

   Purpose

   To make ATG101/ATG13-HORMA and ATG9 C-terminal tail fusion complex for expression in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG9 and ATG101 (ATG9A, ATG101 Human)
   

   Use

   Tags

   GST tag

   Expression

   Mammalian

   Mutation

   codon-optimized ATG9 (828-839) and codon-optimize…

   Promoter

   CMV

   Available since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223_HAVCR2_WT_V5

   Plasmid

   #82965

   Purpose

   Gateway Donor vector containing HAVCR2, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Jesse Boehm, Matthew Meyerson

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   HAVCR2 (HAVCR2 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   R140L

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCI-TPI_WT-IL6_3UTR-xrRNA-4H

   Plasmid

   #108373

   Purpose

   Expresses wild type TPI reporter; contains partial IL6 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

   Depositor

   Niels Gehring

   Article

   Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

   Insert

   Wild type TPI reporter with partial IL6 control 3' UTR, MVE xrRNA and 4H probe binding sites (TPI1, IL6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCI-TPI_WT-RAB7A_3UTR-xrRNA-4H

   Plasmid

   #108372

   Purpose

   Expresses wild type TPI reporter; contains partial RAB7A control 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

   Depositor

   Niels Gehring

   Article

   Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

   Insert

   Wild type TPI reporter with partial RAB7A control 3' UTR, MVE xrRNA and 4H probe binding sites (TPI1, RAB7A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-EML4-ALK variant 1

   Plasmid

   #183828

   Purpose

   Express EML4-ALK fusion variant 1 (E13;A20) in mammalian cells

   Depositor

   Aaron Hata

   Article

   Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

   Inserts

   ALK Receptor Tyrosine Kinase (ALK Human)
   EMAP Like 4 (EML4 Human)
   

   Use

   Lentiviral

   Tags

   The fusion protein of EML4 exon 1-13 and ALK exon…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-EML4-ALK variant 3a

   Plasmid

   #183829

   Purpose

   Express EML4-ALK fusion variant 3a (E6a;A20) in mammalian cells

   Depositor

   Aaron Hata

   Article

   Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

   Inserts

   ALK Receptor Tyrosine Kinase (ALK Human)
   EMAP Like 4 (EML4 Human)
   

   Use

   Lentiviral

   Tags

   The fusion protein of EML4 exon 1-6a and ALK exon…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-EML4-ALK variant 3a G1202R

   Plasmid

   #183830

   Purpose

   Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK G1202R mutation in mammalian cells

   Depositor

   Aaron Hata

   Article

   Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

   Inserts

   ALK Receptor Tyrosine Kinase (ALK Human)
   EMAP Like 4 (EML4 Human)
   

   Use

   Lentiviral

   Tags

   The fusion protein of EML4 exon 1-6a and ALK exon…

   Expression

   Mammalian

   Mutation

   G1202R

   Promoter

   CMV

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-EML4-ALK variant 3a L1196M/G1202R

   Plasmid

   #183831

   Purpose

   Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK L1196M/G1202R mutation in mammalian cells

   Depositor

   Aaron Hata

   Article

   Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

   Inserts

   ALK Receptor Tyrosine Kinase (ALK Human)
   EMAP Like 4 (EML4 Human)
   

   Use

   Lentiviral

   Tags

   The fusion protein of EML4 exon 1-6a and ALK exon…

   Expression

   Mammalian

   Mutation

   L1196M/G1202R

   Promoter

   CMV

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC57KAN-5XHA

    Plasmid

    #233102

    Purpose

    5X HA tag multimer

    Depositor

    Steve Stowers

    Insert

    HA tag
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    March 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUC57KAN-5XV5

    Plasmid

    #233105

    Purpose

    5X V5 tag multimer

    Depositor

    Steve Stowers

    Insert

    V5 tag
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    March 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUC57KAN-20XHA

    Plasmid

    #233114

    Purpose

    20X HA tag multimer

    Depositor

    Steve Stowers

    Insert

    HA tag
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUC57KAN-80XV5

    Plasmid

    #233128

    Purpose

    80X V5 tag multimer

    Depositor

    Steve Stowers

    Insert

    V5 tag
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    March 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUC57KAN-20XV5

    Plasmid

    #233117

    Purpose

    20X V5 tag multimer

    Depositor

    Steve Stowers

    Insert

    V5 tag
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUC57KAN-40XV5

    Plasmid

    #233123

    Purpose

    40X V5 tag multimer

    Depositor

    Steve Stowers

    Insert

    V5 tag
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUC57KAN-10XV5

    Plasmid

    #233111

    Purpose

    10X V5 tag multimer

    Depositor

    Steve Stowers

    Insert

    V5 tag
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUC57KAN-10XHA

    Plasmid

    #233108

    Purpose

    10X HA tag multimer

    Depositor

    Steve Stowers

    Insert

    HA tag
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    March 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC57KAN-5XFLAG

    Plasmid

    #233101

    Purpose

    5X FLAG tag multimer

    Depositor

    Steve Stowers

    Insert

    FLAG tag
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    March 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUC57KAN-5XALFA

    Plasmid

    #233100

    Purpose

    5X ALFA tag multimer

    Depositor

    Steve Stowers

    Insert

    ALFA tag
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    March 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC57KAN-5XMYC

    Plasmid

    #233103

    Purpose

    5X MYC tag multimer

    Depositor

    Steve Stowers

    Insert

    MYC tag
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    March 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge