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Plasmid#166922PurposeBackbone for assembly of multi-level gene expression controllersDepositorInsertCyOFP - Orange fluorescent protein - flanked by BsaI sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23100 constitutiveAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTHS
Plasmid#166938PurposeToehold switch multi-level gene expression controller (MLC)DepositorInsertGenetic circuit expressing GFP- Toehold switch
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPTACAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHES820
Plasmid#89190PurposeExpresses YFP under control of GAL1 promoter.DepositorInsertpGAL1-YFP
UseTagsExpressionYeastMutationPromoterGAL1Available sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHES834
Plasmid#87947PurposeExpresses mKate2 under control of GAL1 promoter.DepositorInsertpGAL1-mKate2
UseSynthetic BiologyTagsExpressionYeastMutationPromoterpGal1Available sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHES854
Plasmid#89197PurposeExpresses mKate2 under control of TDH3 constitutive promoter.DepositorInsertpTDH3-mKate2
UseTagsExpressionYeastMutationPromoterTDH3Available sinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPtac
Plasmid#166937PurposePtac single-level gene expression controller (SLC)DepositorInsertGenetic circuit expressing GFP- PTAC
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPTACAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHES833
Plasmid#90409PurposeExpresses YFP under control of GAL1 promoterDepositorInsertpZ-YFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceNov. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACSA_Pcpt_YFP
Plasmid#82019PurposepALM34; control for YFP expression from cyanobacterial phycocyanin truncated promoterDepositorInsertYFP
UseTagsExpressionMutationPromoterPcptAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR_CMV_MeltTEVp-40-mCherry
Plasmid#234064PurposeEncodes a TEV protease controlled by a Melt variant with a switching temperature of 40°CDepositorInsertMeltTEV-40-mCherry
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-g0
Plasmid#231186PurposeNon-coding DNA plasmid controlDepositorInsertg0
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSTAR2
Plasmid#166940PurposeSTAR-based multi-level gene expression controller (MLC)DepositorInsertCircuit expressing GFP- Small transcription activating RNAs
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPTACAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSTAR
Plasmid#166939PurposeSTAR-based multi-level gene expression controller (MLC)DepositorInsertGenetic circuit expressing GFP- Small Transcription activating RNA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPTACAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pG-04
Plasmid#166935PurposeTarget DC (G)DepositorInsertpT181 dual control activator (DC) - Flanked by BsaI restriction sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSV_238
Plasmid#122941PurposeExpression of the repressor to control the insertional promoter.DepositorInsertrTetR.SUM1
UseTagsExpressionYeastMutationPromoterPret2Available sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHES942
Plasmid#90408PurposeExpresses SIC1 udner control of synthetic promoter containing Zif268 binding sites.DepositorInsertpZ-SIC1
UseTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Lentiviral nontargeting shRNA GFP
Plasmid#155285PurposeLentiviral expression of nontargeting shRNA, GFP expression, based on Addgene 12247DepositorInsertNontargeting shRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
SSpB-HaloTag-p63DH
Plasmid#176116PurposeHeterodimerization with iLID, RhoA activationDepositorInsertRHOGEF p63 (ARHGEF25 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
SSpB-mCherry-p63DH
Plasmid#176112PurposeHeterodimerization with iLID, RhoA activationDepositorInsertRHOGEF p63 (ARHGEF25 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
SSpB-iRFP-p63DH
Plasmid#176120PurposeHeterodimerization with iLID, RhoA activationDepositorInsertRHOGEF p63 (ARHGEF25 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-SspB-HaloTag-p63DH
Plasmid#176129PurposeHeterodimerization with iLID, RhoA activationDepositorInsertRHOGEF p63 (ARHGEF25 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only