We narrowed to 7,391 results for: ef1a;

1. pAAV-Ef1a-fDIO-GCaMP6s

   Plasmid

   #105714

   Purpose

   Can be used to express GCaMP6s in the presence of Flp recombinase. Can be used to create adeno-associated virus to express Flp-dependent GCaMP6s.

   Depositor

   Rylan Larsen

   Has Service

   AAV8

   Insert

   GCaMP6s
   

   Use

   AAV; Flp/frt

   Expression

   Mammalian

   Promoter

   Ef1a, Human elongation factor-1 alpha

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF1a-PEmax-P2A-GFP

   Plasmid

   #202000

   Purpose

   Based on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.

   Depositor

   Daniel Geschwind

   Insert

   PEmax-P2A-GFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-pEF1a-EBFP-tTAn

   Plasmid

   #172122

   Purpose

   Constitutive expression of EBFP and rtTA-InteinN

   Depositor

   Chun Xu

   Article

   Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

   Insert

   EBFP, tTAn-InteinN
   

   Use

   AAV

   Promoter

   EF1a

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOTTC1328 - pAAV EF1a HA-hM3D(Gq)

   Plasmid

   #89149

   Purpose

   An AAV packaging plasmid that expresses HA-tagged hM3D(Gq) excitatory DREADD from the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Gomez et al Science. 2017 Aug 4;357(6350):503-507. d

   Insert

   hM3D(Gq)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAF0084 Ef1a-Cp36-2a-EGFP

   Plasmid

   #193462

   Purpose

   Expression of Cp36 recombinase from Ef1a promoter

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Cp36
   

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV x EF1a_EKAREN4-ires-puro

   Plasmid

   #167828

   Purpose

   Optimized EKAREV FRET biosensor sensor for ERK

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN4
   

   Use

   Lentiviral

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W

   Promoter

   EF1a

   Available Since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPV-EF1a-mRxxFP1(AfeI)-iP-A

   Plasmid

   #204625

   Purpose

   Cloning vector for SSA vector (mRxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestion

   Depositor

   Akitsu Hotta

   Article

   Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOTTC293 - pAAV EF1a V5-synuclein (WT)

   Plasmid

   #60057

   Purpose

   An AAV packaging vector that expresses wildtype alpha-synuclein under control of the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

   Insert

   alpha-synuclein (SNCA Human, human)
   

   Use

   AAV

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV x EF1a_EKAREN5(TA)-ires-puro

   Plasmid

   #167823

   Purpose

   control sensor for EKAREN5

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN5(TA)
   

   Use

   Lentiviral

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W, T420A

   Promoter

   EF1a

   Available Since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. p5E-EF1a (JDW 1164)

    Plasmid

    #224473

    Purpose

    Gateway compatible 5' entry clone containing the human EF1a promoter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    EF1a promoter
    

    Expression

    Mammalian

    Available Since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. EF1a-EGFP (no signal control)

    Plasmid

    #170167

    Purpose

    Mammalian Expression of no signal control

    Depositor

    Christopher Moxham

    Type

    Empty backbone

    Expression

    Mammalian

    Mutation

    n/a

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-Ef1a-fDIO-mCherryP2ACre

    Plasmid

    #161773

    Purpose

    Flp-Dependent mCherry and Cre expression

    Depositor

    Baoji Xu

    Article

    An et al Nat Commun. 2020 Apr 7;11(1):1729. doi:

    Insert

    mCherry-P2A-Cre
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-EF1alpha-dCas9-KRAB_Hygro

    Plasmid

    #192663

    Purpose

    3rd generation lenti vector encoding dCas9-KRAB with 2A Hygro resistance marker

    Depositor

    Jeffrey Miner

    Insert

    dCas9-KRAB
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A, D839A, H840A and N863A in Cas9

    Promoter

    EF1a

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Ef1a-MCP-huADARE488QT490A

    Plasmid

    #159922

    Purpose

    Fusion of MS2 coat protein to Human ADAR2 E488QT490A, under Ef1a promoter, with WPRE. AKA 116v5

    Depositor

    Fei Chen

    Article

    Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

    Insert

    Human ADAR2 E488QT490A (ADARB1 Human)
    

    Use

    AAV

    Promoter

    EF1a

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. hAAVS1-gs4_EF1a-Cas9-2A-GFP

    Plasmid

    #118414

    Purpose

    CRISPR knockout, expresses Cas9, and sgRNA targeting AAVS1 locus

    Depositor

    Mark Denham

    Article

    Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

    Insert

    gRNA AAVS1 (AAVS1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-EF1a-GFP ArgSen

    Plasmid

    #184704

    Purpose

    Lentiviral transfer plasmid for expression of GFP ArgSen

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    GFP ArgSen
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lv EF1a MS2-Fkbp2x 2A Hygro

    Plasmid

    #102807

    Purpose

    Lentiviral plasmid from FIRE-Cas9 system to express MS2-Fkbp2x fusion protein

    Depositor

    Jerry Crabtree

    Article

    Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

    Insert

    MS2-Fkbp2x
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOTTC1329 - pAAV EF1a HA-hM4D(Gi)

    Plasmid

    #89150

    Purpose

    An AAV packaging plasmid that expresses HA-tagged hM4D(Gi) inhibitory DREADD from the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Gomez et al Science. 2017 Aug 4;357(6350):503-507. d

    Insert

    hM4D(Gi)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-Ef1a-fDIO-GCaMP6f

    Plasmid

    #128315

    Purpose

    Expresses GCaMP6f under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

    Depositor

    Patricia Jensen

    Article

    Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

    Has Service

    AAV1

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    His Tag

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLv-EF1a-Parkin-A92mKO2

    Plasmid

    #213559

    Purpose

    Lentiviral vector for stable expression of Parkin, internally tagged with mKO2

    Depositor

    Steven Altschuler, Lani Wu

    Article

    Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

    Insert

    Parkin (PRKN Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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