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pCDNA3-FLAG-Regnase-3-HA_full-length

Plasmid

#222656

Purpose

Mammalian expression vector to express full-length Regnase-3 tagged with FLAG at N-term and HA at C-term

Depositor

Silvia Monticelli

Publication

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c (Zc3h12c Mouse)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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Beta1 Q280* K418* full-length

Plasmid

#221406

Purpose

Mammalian expression of human integrin beta1 Q280* K418* full-length

Depositor

Timothy Springer

Publication

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 Q280* K418* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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Beta1 N391* N562* full-length

Plasmid

#221407

Purpose

Mammalian expression of human integrin beta1 N391* N562* full-length

Depositor

Timothy Springer

Publication

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 N391* N562* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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Fusion of Dengue2 NS2B-NS3 proteins

Plasmid

#204793

Purpose

Bacterial expression of a fusion of Dengue2 NS2B-NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

Dengue2 NS2B-NS3 fusion

Expression

Bacterial

Available since

Jan. 17, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV(WT)-mouse-full-length-CHMP3-HA

Plasmid

#154175

Purpose

expresses mouse CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Publication

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3 (Chmp3 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-VSVM

Plasmid

#64086

Purpose

Expression vector for Vesicular Stomatitis Virus Indiana strain matrix gene

Depositor

Ian Wickersham

Insert

VSV matrix protein

Expression

Mammalian

Promoter

CAG

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pK18msB

Plasmid

#177839

Purpose

Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size.

Depositor

Christopher Johnson

Publication

Ling et al Nat Commun. 2022 Aug 22;13(1):4925. doi:

Type

Empty backbone

Use

Bacterial gene replacement

Available since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJRH-1180 U6-reci Gag-pol v2

Plasmid

#201915

Purpose

Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

Depositor

Jennifer Doudna

Publication

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Gag-pol

Expression

Mammalian

Promoter

CAG

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p37-2iDMD-LR

Plasmid

#88892

Purpose

37°C full-length DMD luc-red plasmid. Co-expresses human dystrophin, firefly luciferase and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites and can be grown at 37°C.

Depositor

Michele Calos

Publication

Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/

Inserts

Dystrophin (DMD Human)
luciferase
mCherry

Expression

Mammalian

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAVS1-P-CAG-mCh

Plasmid

#80492

Purpose

donor vector for AAVS1 targeting (puromycin selection) and constitutive mCherry expression

Depositor

Knut Woltjen

Publication

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

mCherry

Use

Donor vector (human)

Expression

Mammalian

Promoter

CAG

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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3X FLAG-FUS-WT

Plasmid

#44985

Depositor

Robin Reed

Publication

Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

Insert

FUS (Full length) (FUS Human)

Tags

3X FLAG

Expression

Mammalian

Promoter

CMV

Available since

July 1, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA 3.1(-) VSFP3.1

Plasmid

#18951

Depositor

Thomas Knopfel

Publication

Lundby et al PLoS ONE. 2008 . 3(6):e2514.

Insert

VSFP3.1

Expression

Mammalian

Available since

Sept. 30, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Lenti-TurboCas-NLS-Blast

Plasmid

#232346

Purpose

Lentiviral expression vector for dCas9 fused to miniTurboNLS

Depositor

Ali Shilatifard

Publication

Cenik et al Mol Cell. 2024 Dec 19;84(24):4929-4944.e

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xHA, miniTurbo, V5 and 3xSV40 NLS

Expression

Mammalian

Promoter

EF1a

Available since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMLM36 (aka pBR-UV5-GP-FD2)

Plasmid

#21871

Depositor

Keith Joung

Publication

Maeder et al Nat Protoc. 2009. 4(10):1471-501.

Type

Empty backbone

Expression

Bacterial

Available since

Nov. 3, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pX601-mCherry

Plasmid

#84039

Purpose

Staphylococcus aureus (SaCas9) conjugated with mCherry

Depositor

Yuet Wai Kan

Publication

Ye et al Proc Natl Acad Sci U S A. 2016 Sep 20;11

Insert

SaCas9

Use

AAV and CRISPR

Tags

NLS and T2A-mCherry

Expression

Mammalian

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pXG260

Plasmid

#141282

Purpose

CMV:EGFP (as control for pXG237)

Depositor

Martin Kampmann

Publication

Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

Insert

EGFP

Use

Lentiviral

Promoter

CMV

Available since

Sept. 19, 2020

Availability

Academic Institutions and Nonprofits only

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scFLARE2

Plasmid

#158700

Purpose

Neuronal expression of scFLARE2 component

Depositor

Alice Ting

Publication

Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

Insert

scFLARE2

Use

AAV

Tags

HA, Flag, VP16

Expression

Mammalian

Mutation

Truncated form of TEV protease (220-242 amino aci…

Promoter

Syn

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Cas9-2A-Cre

Plasmid

#68468

Purpose

co-expressing Cas9 and Cre recombinase

Depositor

Su-Chun Zhang

Publication

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Insert

Cas9 and Cre recombinase

Use

CRISPR

Tags

P2A-Cre-Myc

Expression

Mammalian

Promoter

CAG

Available since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCVL SFFV-EF1s HA.NLS.Sce(opt)

Plasmid

#31479

Depositor

Andrew Scharenberg

Publication

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV/Ef1s HA NLS I-SceI

Use

Lentiviral

Tags

HA and NLS

Mutation

"opt" indicates the coding sequence has…

Available since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3-HA PTEN

Plasmid

#78776

Purpose

To overexpress PTEN in Mammalian Cells

Depositor

Jaewhan Song

Publication

Lee et al Nat Commun. 2015 Jul 17;6:7769. doi: 10.

Insert

PTEN (PTEN Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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