We narrowed to 5,524 results for: pcas

1. pCas9-CAT

   Plasmid

   #80323

   Purpose

   Encodes Cas9 and chloramphenicol acetyltransferase (CAT)

   Depositor

   Sebastian Lourido

   Article

   Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

   Inserts

   Cas9
   Chloramphenicol acetyltransferase
   

   Use

   CRISPR

   Tags

   3X FLAG and NLS

   Expression

   Mutation

   Promoter

   TgTUB1

   Available since

   Sept. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSAVED-CHAT_PCaspase_PCi_PC-σ

   Plasmid

   #214040

   Purpose

   Bacterial expression plasmid for SAVED-CHAT, PCaspase, PCi, and PC-σ from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT-Pcaspase-Pci-PC-σ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   lacUV5 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS-AcGFP-C

   Plasmid

   #203325

   Purpose

   Express N-terminal AcGFP-fused protein in mammalian cell

   Depositor

   Chiaki Murakami

   Article

   Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

   Type

   Empty backbone

   Use

   Tags

   AcGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCALNL-tdTomato

   Plasmid

   #194205

   Purpose

   Cre/LoxP-dependent expression of a tdTomato. Expression of Cre excises a STOP codon before tdTomato, facilitating fluorescence expression of tdTomato.

   Depositor

   Angelique Bordey

   Insert

   tdTomato
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-tdTHC

   Plasmid

   #112621

   Purpose

   expression plasmid with CAG promoter driving multicistronic cassette H2BtdTomato_p2A_HygroR_p2A_CreERt2

   Depositor

   Stefan Heller

   Article

   Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

   Inserts

   H2B-tdTomato (H2BC3 Human, Anthozoa)
   Hygrophosphotransferase
   Cre-ERT2 fusion protein
   

   Use

   Cre/Lox

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS-6020nes

   Plasmid

   #108656

   Purpose

   A JNK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

   Depositor

   Michiyuki Matsuda

   Article

   Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

   Insert

   hyBRET-JNK-EV
   

   Use

   Tags

   XFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-HArcLight

   Plasmid

   #138326

   Purpose

   chemigenetic voltage indicator

   Depositor

   Eric Schreiter

   Article

   Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

   Insert

   HArcLight
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG/ATP9B-HA

   Plasmid

   #209214

   Purpose

   Mammalian expression of ATP9B

   Depositor

   Hye-Won Shin

   Article

   Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

   Insert

   ATP9B (ATP9B Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-B19M

   Plasmid

   #59923

   Purpose

   Expression vector for Rabies virus SAD B19 matrix protein gene

   Depositor

   Ian Wickersham

   Insert

   Rabies virus matrix protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-GFPmG1

    Plasmid

    #50444

    Purpose

    Expression of a EGFP variant in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    GFPmG1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E143K, N147Q

    Promoter

    CAG

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCas9-VRER_2A_GFP

    Plasmid

    #75475

    Purpose

    Cas9 VRER variant that detects NGCG PAM, combined with 2A_GFP for expression control

    Depositor

    Marc Tessier-Lavigne

    Article

    Paquet et al Nature. 2016 May 5;533(7601):125-9. doi:

    Insert

    Cas9-VRER
    

    Use

    CRISPR

    Tags

    2A_GFP

    Expression

    Mammalian

    Mutation

    D1135V, G1218R, R1335E, T1337R

    Promoter

    CAG

    Available since

    May 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-Bmp5

    Plasmid

    #163589

    Purpose

    Expresses BMP-5 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmp5 (Bmp5 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-i53

    Plasmid

    #119002

    Purpose

    Mammalian expression of i53 (53BP1) inhibitor and BFP

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    i53
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMA-SpCas9-g9

    Plasmid

    #80792

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 9/19/29 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMA-SpCas9-g8

    Plasmid

    #80791

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 8/18/28 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. 2X_pX458_pSpCas9(BB)-2A-GFP_SOX17_bKO

    Plasmid

    #172225

    Purpose

    Cas9-2A-GFP expression vector bearing two sgRNAs targeting the centromeric human SOX17 CTCF-boundary.

    Depositor

    Alexander Meissner

    Article

    Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

    Insert

    sgRNA
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pF(UG) hSyn spCas9

    Plasmid

    #179725

    Purpose

    human synapsin (hSyn)-driven spCas9 lentiviral vector for CRISPR/HITI

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    spCas9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. SpCas9 iFE

    Plasmid

    #190138

    Purpose

    Expresses SpCas9-POLB (iFE)

    Depositor

    Timothy Lu

    Article

    Nakade et al bioRxiv 2022.12.05.518807

    Insert

    SpCas9-POLB (HiFi iFE)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Codon Optimized for mammalian cells

    Promoter

    CBh

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAMKIIa-TetOn3G

    Plasmid

    #133727

    Purpose

    Plasmid contains the TetOn3G-transactivator sequence under control of a Camk2a promoter.

    Depositor

    Erin Schuman

    Article

    Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

    Insert

    TetOn3G
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCamk2a

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAD104_Asn152Ser_pCMV6Entry-hIRG1

    Plasmid

    #124877

    Purpose

    Expresses hCAD Asp152Ser mutant in mammalian cells

    Depositor

    Konrad Buessow

    Article

    Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

    Insert

    cis-aconitate decarboxylase (ACOD1 Human)
    

    Use

    Tags

    myc and FLAG tags

    Expression

    Mammalian

    Mutation

    mutated Asn152 to Ser

    Promoter

    CMV

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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