We narrowed to 7,134 results for: tev

1. pETDuet-1_6xHis-TEV-mCh-NDP52

   Plasmid

   #187829

   Purpose

   Plasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   NDP52 (CALCOCO2 Human)
   

   Use

   Tags

   6xHisTag, TEV, and mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCD5-PR8-TEV-sfGFP-twinStrep

   Plasmid

   #157933

   Purpose

   Expresses influenza A virus trimeric hemagglutinin fused to a sfGFP

   Depositor

   Robert de Vries

   Article

   Nemanichvili et al J Mol Biol. 2019 Feb 15;431(4):842-856.

   Insert

   A/PuertoRico/8/34 hemagglutinin (HA Synthetic)
   

   Use

   Tags

   sfGFP & TwinStrep

   Expression

   Mammalian

   Mutation

   E190D

   Promoter

   CMV

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Gal:mCherry-CRY2-uTEV1Δ(220-242)

   Plasmid

   #135452

   Purpose

   Yeast expression of uTEV1Δ fused to CRY2 and mCherry

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   mCherry-CRY2-TEV1Δ(220-242)
   

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Promoter

   Gal

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCD5-H3CH13-CO-GCN4-TEV-sfGFP-TS

   Plasmid

   #158740

   Purpose

   Expresses influenza A virus trimeric hemagglutinin A/H3/CH/13 fused to a sfGFP

   Depositor

   Robert de Vries

   Article

   van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

   Insert

   Hemagglutinin A/MI/45/15 H1N1
   

   Use

   Tags

   GCN4-TEV-sfGFP-TwinStrep

   Expression

   Mammalian

   Mutation

   AA61-469

   Promoter

   CMV

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET51b-His-TEV-CLIP-tag_fast

   Plasmid

   #167275

   Purpose

   Overexpression of the fast variant (E30R) of CLIP-tag in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   CLIPf-tag
   

   Use

   Tags

   His x10

   Expression

   Bacterial

   Mutation

   E30R

   Promoter

   T7

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFl Strep-sumo-TEV-hAgo2

   Plasmid

   #136235

   Purpose

   Expresses human Argonaute-2 in insect cells

   Depositor

   Leemor Joshua-Tor

   Article

   Elkayam et al Cell. 2012 Jul 6;150(1):100-10. doi: 10.

   Insert

   human Argonaute-2 (AGO2 Human)
   

   Use

   Tags

   Strep Sumo

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   May 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-Duet1_6xHis-TEV-eGFP-P62

   Plasmid

   #190929

   Purpose

   Plasmid for the expression and purification of GFP labelled p62. Internal reference: SMC390

   Depositor

   Sascha Martens

   Article

   Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

   Insert

   P62 (SQSTM1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV:eGFP-CaM-uTEV1Δ(220-242)

   Plasmid

   #135461

   Purpose

   Mammalian expression of uFLARE protease component

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   eGFP-CaM-uTEV1Δ
   

   Use

   Tags

   eGFP, V5

   Expression

   Mammalian

   Mutation

   S219V mutation improves stability and S153N impro…

   Promoter

   CMV

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-TRE-DIO-Casp3-TEV

   Plasmid

   #183766

   Purpose

   This plasmid is for use with neuronal cell-type selective activity tagging. The Caspase3 expression requires both neural activity and Cre recombinase.

   Depositor

   William Wisden

   Article

   Yu et al Science. 2022 Jul;377(6601):63-72. doi:

   Insert

   AAV transgene - tetO promoter-flex/DIO-Caspase3-TEV
   

   Use

   AAV, Cre/Lox, and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO promoter

   Available since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCD5-TS-sfGFP-Tetrabrachion-TEV-N2NL16

    Plasmid

    #158742

    Purpose

    Expresses influenza A virus tetrameric neuraminidase fused to a sfGFP

    Depositor

    Robert de Vries

    Article

    van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

    Insert

    Neuraminidase A/NL/354/16 H3N2
    

    Use

    Tags

    TwinStrep_sfGFP_tetrabrachion

    Expression

    Mammalian

    Mutation

    AA61-469

    Promoter

    CMV

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET His6 MBP TEV HDAC8_374

    Plasmid

    #122174

    Purpose

    Expresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tag

    Depositor

    David Christianson

    Article

    Porter et al ACS Chem Biol. 2017 Sep 15;12(9):2281-22

    Insert

    His6 MBP TEV HDAC8_374 (HDAC8 Human)
    

    Use

    Tags

    hexahistidine tag and tobacco etch virus protease…

    Expression

    Bacterial

    Mutation

    Residues Met1–Pro7 and H375-V377 have been delete…

    Promoter

    T7

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-mTagBFP2-TEV-Strep-His

    Plasmid

    #210824

    Purpose

    Bacterial overexpression vector for C-terminally Strep and His tagged mTagBFP2 fluorescent protein

    Depositor

    Luke Chao

    Article

    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

    Insert

    mTagBFP2
    

    Use

    Tags

    TwinStrep, 10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-Golgin-84-TEV #709#243

    Plasmid

    #42108

    Depositor

    Ayano Satoh

    Article

    Koreishi et al Antibody Technology Journal. 2011;1:5-11

    Insert

    Golgin-84 (GOLGA5 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    inserted ENLYFQG b/w 300 and 301

    Promoter

    CMV

    Available since

    Feb. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pET28 E2 LBD-Tev-E1a-His

    Plasmid

    #232117

    Purpose

    For bacterial expression of a fusion protein containing from N to C-terminus: the lipoyl-binding domain of Human E2, a TEV protease site, a peptide from Human E1a, and a His tag.

    Depositor

    Ruth Sommese

    Article

    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

    Insert

    DBT (DBT Human)
    

    Use

    Tags

    6xHis, RIGHHSTSDDSSAY (AA331 to 345 (preprocessin…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFA6a-hphMX-(3×FLAG)-TEV-ProtA

    Plasmid

    #52692

    Purpose

    For C-terminal tagging of ORFs with a (3×FLAG)-TEV-ProtA

    Depositor

    Michael Nick Boddy

    Insert

    (3×FLAG)-TEV-ProtA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    April 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. His-FLAG-Tev_ACTA1 (alpha-actin)

    Plasmid

    #188452

    Purpose

    Express in mammalian cells human alpha-actin (ACTA1) with N-terminal 6xHis tag, FLAG tag, and TEV protease site.

    Depositor

    Roberto Dominguez

    Article

    Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

    Insert

    ACTA1 (ACTA1 Human)
    

    Use

    Tags

    6xHis-FLAG

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKK-RNAi-nucEGFPmiR-TEV-mCherry

    Plasmid

    #105812

    Purpose

    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with C-terminal mCherry.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    RNAi; Flp-in competent

    Tags

    TEV-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1 CARD8-TEV-GFP-FLAG

    Plasmid

    #169991

    Purpose

    Mammalian purification construct for CARD8

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    TEV-GFP-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. 6xHis(TEV)-Drp1(K38A)-StrepII

    Plasmid

    #174423

    Purpose

    Expresses human Drp1 Isoform 3 with K38A mutation in bacteria

    Depositor

    Thomas Pucadyil

    Article

    Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

    Insert

    Drp1
    

    Use

    Tags

    6xHis(TEV) and StrepII

    Expression

    Bacterial

    Mutation

    K38A

    Promoter

    T7

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. HA-MBP-TEVpCS-ADGRB1 CTF-FLAG

    Plasmid

    #217681

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRB1 (ADGRB1 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-926

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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