292,743 results

1. PH-PLCD1-GFP

   Plasmid

   #51407

   Purpose

   Is a biosensor for PI(4,5)P2

   Depositor

   Tamas Balla

   Article

   Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

   Insert

   PH-PLCD1 (aa 1–170) (PLCD1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. mCherry-Vinculin-23

   Plasmid

   #55159

   Purpose

   Localization: Focal Adhesions, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Vinculin (VCL Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mCherry-Vinculin-23

   Plasmid

   #55159

   Purpose

   Localization: Focal Adhesions, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Vinculin (VCL Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40

   Plasmid

   #100853

   Purpose

   AAV expression of Cre recombinase-activated jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Has Service

   AAV Retrograde and AAV1

   Insert

   jRGECO1a
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40

   Plasmid

   #100853

   Purpose

   AAV expression of Cre recombinase-activated jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Has Service

   AAV Retrograde and AAV1

   Insert

   jRGECO1a
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBABE_hygro_mRFP1_NRF2

   Plasmid

   #136579

   Purpose

   mammalian expression of mRFP1-tagged human NRF2

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   NRF2 (NFE2L2 Human)
   

   Use

   Retroviral

   Tags

   N-mRFP1-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBABE_hygro_mRFP1_NRF2

   Plasmid

   #136579

   Purpose

   mammalian expression of mRFP1-tagged human NRF2

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   NRF2 (NFE2L2 Human)
   

   Use

   Retroviral

   Tags

   N-mRFP1-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHDR-USR-SpCas9

   Plasmid

   #208829

   Purpose

   HDR Universal Surrogate Reporter with a sgRNA and consistently expressed SpCas9. The PuroR reporter gene was designed to be repaired as a selecting marker gene only by the sgRNA/Cas9-triggered HDR.

   Depositor

   Zhiying Zhang

   Article

   Yan et al Mol Ther Nucleic Acids. 2020 Mar 6;19:77

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHR_5x Gal4 UAS

   Plasmid

   #79119

   Purpose

   Base vector for cloning Gal4DBDVP64 inducible genes

   Depositor

   Wendell Lim

   Article

   Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHR_5x Gal4 UAS

    Plasmid

    #79119

    Purpose

    Base vector for cloning Gal4DBDVP64 inducible genes

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pVasa-Cre

    Plasmid

    #15885

    Depositor

    Diego Castrillon

    Article

    Gallardo et al Genesis. 2007 Jun . 45(6):413-7.

    Insert

    mouse vasa promoter (Ddx4 Mouse)
    

    Use

    Tags

    Cre

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pVasa-Cre

    Plasmid

    #15885

    Depositor

    Diego Castrillon

    Article

    Gallardo et al Genesis. 2007 Jun . 45(6):413-7.

    Insert

    mouse vasa promoter (Ddx4 Mouse)
    

    Use

    Tags

    Cre

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pVDL9.3

    Plasmid

    #168299

    Purpose

    Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA

    Depositor

    Víctor de Lorenzo

    Article

    Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do

    Insert

    Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LacI-Plac

    Available since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pVDL9.3

    Plasmid

    #168299

    Purpose

    Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA

    Depositor

    Víctor de Lorenzo

    Article

    Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do

    Insert

    Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LacI-Plac

    Available since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LV-Sox10MCS5-GFP

    Plasmid

    #115783

    Purpose

    This plasmid encodes for Sox10-MCS5-GFP reporter. Cell carrying this construct expresses green fluorescence under the control of SOX-MCS5 enhancer conjugated with cfos basal promoter.

    Depositor

    Fraser Sim

    Article

    Pol et al Exp Neurol. 2013 Sep;247:694-702. doi: 1

    Insert

    mouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
    

    Use

    Lentiviral

    Tags

    cfos basal promoter conjugated MCS5 promoter fuse…

    Expression

    Mutation

    Promoter

    SOX10 Multiple Species Conserved enhancer element…

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LV-Sox10MCS5-GFP

    Plasmid

    #115783

    Purpose

    This plasmid encodes for Sox10-MCS5-GFP reporter. Cell carrying this construct expresses green fluorescence under the control of SOX-MCS5 enhancer conjugated with cfos basal promoter.

    Depositor

    Fraser Sim

    Article

    Pol et al Exp Neurol. 2013 Sep;247:694-702. doi: 1

    Insert

    mouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
    

    Use

    Lentiviral

    Tags

    cfos basal promoter conjugated MCS5 promoter fuse…

    Expression

    Mutation

    Promoter

    SOX10 Multiple Species Conserved enhancer element…

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGLS3-5xHRE-p53(K164R)

    Plasmid

    #72555

    Purpose

    Hypoxia induced mutant p53. Contains the HRE from VEGF sequence (5 repeats) upstream of p53 K164R.

    Depositor

    Ester Hammond

    Article

    Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    5XHRE

    Expression

    Mammalian

    Mutation

    K164R

    Promoter

    E1B minimal promoter

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGLS3-5xHRE-p53(K164R)

    Plasmid

    #72555

    Purpose

    Hypoxia induced mutant p53. Contains the HRE from VEGF sequence (5 repeats) upstream of p53 K164R.

    Depositor

    Ester Hammond

    Article

    Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    5XHRE

    Expression

    Mammalian

    Mutation

    K164R

    Promoter

    E1B minimal promoter

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti CMV Puro DEST (w118-1)

    Plasmid

    #17452

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Puro

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti CMV Puro DEST (w118-1)

    Plasmid

    #17452

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Puro

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge