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pDNR-mCherry-T2A-3xPuroR-Frame 2

Plasmid

#206993

Purpose

Donor plasmid for Frame 2 with mCherry tag and triple Puromycin resistant marker (3xPuroR)

Depositor

Alejandro Chavez

Article

Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

mCherry

Expression

Mutation

Promoter

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pEF-XMAS-1xStop

Plasmid

#164411

Purpose

Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 1 stop codon.

Depositor

Xiao Wang

Article

Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

Insert

mCherry

Use

CRISPR and Synthetic Biology

Tags

T2A-GFP

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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His-GFP-Clathrin

Plasmid

#170858

Purpose

Mammalian expression of His-GFP-Clathrin.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Clathrin light polypeptide (Lca) (Clta Mouse)

Use

Tags

(His)6 and EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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Cavb3

Plasmid

#26574

Depositor

Diane Lipscombe

Insert

calcium channel beta subunit-III (Cacnb3 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-hGSDME

Plasmid

#154876

Purpose

Expresses hGSDME in mammalian cells

Depositor

Judy Lieberman

Article

Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

Insert

GSDME (GSDME Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 KP.2

Plasmid

#223273

Purpose

Encodes SARS-CoV-2 spike KP.2 for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike KP.2 (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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PEW-pEF1A-inactEGFP

Plasmid

#212009

Purpose

Cleavable backbone for constitutive expression of TCR alpha and beta chains through lentiviral transduction

Depositor

Catherine Wu

Article

Afeyan et al Nat Protoc. 2024 Nov 8. doi: 10.1038/s41

Insert

Inactivated eGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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psfTn5

Plasmid

#79107

Purpose

bacterial expression of hyperactive Tn5 transposase carrying E54K and L372P mutations

Depositor

Sten Linnarsson

Insert

transposase 5

Use

Tags

His

Expression

Bacterial

Mutation

E54K and L372P

Promoter

Available since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCDH-puro-CMV-RC3AI

Plasmid

#78908

Purpose

Indicator of caspase-3-like protease activity

Depositor

Binghui Li

Article

Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

Insert

RC3AI

Use

Lentiviral

Tags

HA

Expression

Mutation

S320A, deletion of AA1-200

Promoter

CMV

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.3_SOX2

Plasmid

#26817

Depositor

Derrick Rossi

Article

Warren et al Cell Stem Cell. 2010 Sep 29. ():.

Insert

5'UTR-SOX2-3'UTR (SOX2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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hMLKL-Venus

Plasmid

#106078

Purpose

expression in mammalian cells

Depositor

Douglas Green

Article

Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

Insert

human MLKL-Venus (MLKL Human)

Use

Retroviral; Tet-on retrovirus

Tags

Venus

Expression

Mammalian

Mutation

Promoter

Dox-inducible

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-mCh-Cry2olig

Plasmid

#101222

Purpose

Cry2olig(E490G)

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

Insert

Cry2olig

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

E490G

Promoter

SFFV

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pBEST-OR2-OR1-Pr-araC, pBAD-TetR, pBAD-TetO1-deGFP-ssrA

Plasmid

#45789

Depositor

Richard Murray

Inserts

deGFP
Tetracycline repressor
araC

Use

Synthetic Biology

Tags

ssrA degradation tag

Expression

Bacterial

Mutation

Promoter

OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

Available since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pGCS-N2(3xHA)

Plasmid

#85719

Purpose

Gateway destination vector

Depositor

Hai-Ning Du

Article

Wang et al Acta Biochim Biophys Sin (Shanghai). 201

Type

Empty backbone

Use

Mammalian/avian/xenopus/zebrafish

Tags

3xHA

Expression

Mutation

Promoter

Available since

Jan. 9, 2017

Availability

Academic Institutions and Nonprofits only

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sgRNA(MS2) cloning backbone

Plasmid

#61424

Purpose

sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pMK391 (mAID-BSD)

Plasmid

#121192

Purpose

C-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

mAID-BSD

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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WT Smarca4-sfGFP

Plasmid

#107056

Purpose

Expression of wild-type Smarca4 (Brg1) with C-terminal super-folder GFP fusion.

Depositor

Courtney Hodges, Jerry Crabtree

Article

Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

Insert

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)

Use

Lentiviral

Tags

Super-folder GFP (sfGFP)

Expression

Mammalian

Mutation

wild-type

Promoter

CAG

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pMH-Halo tag

Plasmid

#154144

Purpose

Gateway compatible mammalian expression plasmid for halo tag fusion proteins

Depositor

Michael Huen

Type

Empty backbone

Use

Tags

Halo tag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pSLQ9757: CMV-BPNLS_TadA-linkerA-TadA*(8e)-Linker B-SV40 NLS-dCasMINI-V4-linkerC-c-Myc_NLS-3x FLAG-polyA

Plasmid

#176270

Purpose

Expression of TadA-TadA* (8e)-dCasMINI-V4 in mammalian cells

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

TadA-TadA*(8e)-dCasMINI-V4

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-mSmo

Plasmid

#25395

Depositor

Philip Beachy

Article

Chen et al Genes Dev. 2002 Nov 1. 16(21):2743-8.

Insert

mouse Smoothened cDNA (Smo Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2010

Availability

Academic Institutions and Nonprofits only

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