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We narrowed to 14,067 results for: LEA

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Showing: 6801 - 6820 of 14067 results

  4. pJRoC30_Th3.14

    Plasmid
    #188068
    Purpose
    FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.14)
    Depositor
    Sarel Fleishman
    Publication
    Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173
    Insert
    FuncLib-designed high-redox potential laccase from Trametes hirsuta
    Tags
    S. cerevisiae evolved α-factor prepro-leader sign…
    Expression
    Yeast
    Mutation
    20 PROSS + 4 FuncLib mutations, described in publ…
    Available since
    Oct. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    188068-plasmid-map-sequence-id-370141

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  5. pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE

    Plasmid
    #141236
    Purpose
    AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of jRGECO1a (jRGECO1a will not be expressed in mammalian cells that express Cre recombinase)
    Depositor
    Guohong Cui
    Publication
    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do
    Insert
    NES-jRGECO1a
    Use
    AAV and Cre/Lox; Cre-off
    Tags
    6xHIS tag and nuclear export signal
    Promoter
    human elongation factor-1 alpha (EF-1 alpha)
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    141236-plasmid-map-sequence-id-432423

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  6. pcDNA3-EGFP-AC8(1-106)

    Plasmid
    #181958
    Purpose
    Residues 1-106 of rat adenylyl cyclase 8 (AC8) tagged with EGFP.
    Depositor
    Jin Zhang
    Publication
    Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP-AC8(1-106) (Adcy8 Rat)
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    181958-plasmid-map-sequence-id-363198

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  7. t1-405(del37/GAG)

    Plasmid
    #166129
    Purpose
    Expression of human talin-1 head (aa1-405) in E. coli. Contains 37 amino acid deletion in F1-loop, replaced with Gly-Ala-Gly. N-terminal His6, Xpress-epitopeDLYDDDDK) and enterokinase cleavage site.
    Depositor
    Vesa Hytönen
    Publication
    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13
    Insert
    T1head1-405(del37/GAG) (TLN1 Human)
    Tags
    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…
    Expression
    Bacterial
    Mutation
    aa1-405, with 37 aa deletion in the F1-loop which…
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166129-plasmid-map-sequence-id-330258

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  8. pcDNA3.1-V5H6-mPCSK3 D153A

    Plasmid
    #122681
    Purpose
    Expresses mutated mouse PCSK3 in mammalian cells (aspartic acid at position 153 mutated to alanine via site directed mutagenesis)
    Depositor
    Liming Pei
    Publication
    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
    Expression
    Mammalian
    Mutation
    changed Aspartic Acid 153 to Alanine
    Available since
    May 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    122681-plasmid-map-sequence-id-244746

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  9. pLENTICRISPR V2 sgCGAS_1

    Plasmid
    #241454
    Purpose
    Delete CGAS
    Depositor
    Richard Possemato
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgCGAS_1 (CGAS Synthetic)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Nov. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    241454-plasmid-map-sequence-id-492099

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  10. pCMV-T7-SpCas9(MRRCQR)-P2A-EGFP (RAS2823)

    Plasmid
    #223072
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRRCQR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(MRRCQR)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(MRRCQR)=D1135M, S1136R, G1218R, E1219C, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223072-plasmid-map-sequence-id-447672

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  11. pBIG2ab_nsp14/nsp10-6His-3xFlag (SARS-CoV-2)

    Plasmid
    #169164
    Purpose
    Baculoviral transfer vector to co-express SARS-CoV-2 nsp14 and nsp10 in insect cells
    Depositor
    John Diffley
    Publication
    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464
    Insert
    nsp14/nsp10-6His-3xFlag (ORF1ab Synthetic, SARS-CoV-2)
    Tags
    6His-3xFlag (nsp10 C-terminus)
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169164-plasmid-map-sequence-id-333982

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  12. pcDNA3-AMPKsub-YPet-Kras

    Plasmid
    #84638
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Publication
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AMPKsub-YPet-Kras
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    84638-plasmid-map-sequence-id-176882

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  13. pcDNA3-Venus-Venus-FLARE-AKAR

    Plasmid
    #123331
    Purpose
    Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.
    Depositor
    Jin Zhang
    Publication
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Venus-Venus-FLARE-AKAR
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, and Xpress…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    123331-plasmid-map-sequence-id-306914

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  14. pMT-zic2a-NLS-BirA-2A-mCherry_Ras

    Plasmid
    #80065
    Purpose
    Zic2a promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    Zic2a enhancer driving c-fos promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80065-plasmid-map-sequence-id-152617

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  19. pcDNA3.1-Gαi1-Gβ1-Gγ2-GFP2

    Plasmid
    #254719
    Purpose
    Expresses G protein heterotrimer with Gαi1, Gβ1 and Gγ2-GFP2 fusion. Vector used for G protein recruitment or 'nuc-BRET' assay. Based on TRUPATH Triple Gαi1 (#196048).
    Depositor
    Cornelius Gati
    Publication
    Khan et al Nature. 2025 Dec;648(8094):755-763. doi:
    Inserts
    Gαi1 (GNAI1 Human)
    Gβ1 (GNB1 Human)
    Gγ2 (GNG2 Human)
    Tags
    GFP2
    Expression
    Mammalian
    Promoter
    CMV, IRES, and T2A
    Available since
    May 12, 2026
    Availability
    Academic Institutions and Nonprofits only
    254719-plasmid-map-sequence-id-509988

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Showing: 6801 - 6820 of 14067 results