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psiCHECK2-AS34a

Plasmid

#37099

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

perfect complementary binding site for hsa-miR-34a (MIR34A Human)

Use

Luciferase

Available Since

June 19, 2012

Availability

Academic Institutions and Nonprofits only
pET15b-pknG

Plasmid

#102814

Purpose

Expression of PknG in E.coli

Depositor

Jean Pieters

Article

Walburger et al Science. 2004 Jun 18;304(5678):1800-4. E

Insert

pknG

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA Set9 wt

Plasmid

#24084

Depositor

Danny Reinberg

Article

Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

Insert

Set9 (SETD7 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only

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EGFP-PIK3CD

Plasmid

#174190

Purpose

Expresses PIK3CD in mammalian cells

Depositor

Cheng-han Yu

Article

Zhou et al J Cell Sci. 2020 Jun 8;133(11). pii: jcs

Insert

PIK3CD (PIK3CD Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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G417A_pLenti-Tet-BattP(GA)-BFP-2A-iCasp9-2A-Blast_rtTA3

Plasmid

#200631

Purpose

Negative selection blasticidin resistant Tet-inducible Matreyek Bxb1(GA) landing pad

Depositor

Kenneth Matreyek

Article

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

iCasp9

Use

Lentiviral

Promoter

pTRE3G

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(-30)

Plasmid

#199167

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(-30)

Tags

MGHHHHHHGGA

Mutation

T19D, K36E, N49E, K51E, K62E, R83D, K89D, N159D, …

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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anxA1-GFP

Plasmid

#107194

Purpose

Expresses porcine annexin A1-GFP fusion protein for live cell imaging

Depositor

Volker Gerke, Ursula Rescher

Article

Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

Insert

annexin A1

Tags

Green Fluorescent Protein

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR APLP1 shRNA 2

Plasmid

#30157

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al Neural Dev. 2008 Jun 23;3:15. doi: 10.11

Insert

APLP1 shRNA 2

Expression

Mammalian

Available Since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
pRDA_426

Plasmid

#179097

Purpose

BE Cas expression

Depositor

John Doench, David Root

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

ABE8e

Use

CRISPR and Lentiviral

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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px459V2.0_Conc2

Plasmid

#134451

Purpose

Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + Puro

Depositor

Daniel Stange

Article

Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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PB-GFP1-10-HygR

Plasmid

#223675

Purpose

To check the split GFP signals in mitochondria (1)

Depositor

Juan Melero-Martin

Article

Lin et al Nature. 2024 May;629(8012):660-668. doi:

Insert

GFP1-10

Expression

Mammalian

Promoter

cmv

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-AKAP-mtagBFP2-UBC6

Plasmid

#105007

Purpose

Mammalian Expression of mtagBFP2

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

mtagBFP2

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLB1080

Plasmid

#37826

Depositor

Ann Hubbard

Article

Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

Insert

ATP7B (ATP7B Human)

Tags

mEGFP

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pLKO-RFP-shCntrl

Plasmid

#69040

Purpose

Lentiviral expression of control shRNA

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

control shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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mEGFP-PLIN3

Plasmid

#222426

Purpose

To express PLIN3 with mEGFP tag in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

PLIN3 (PLIN3 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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stathmin-GFP

Plasmid

#86782

Purpose

Mammalian expression of stathmin-GFP

Depositor

Lynne Cassimeris

Article

Ringhoff et al Mol Biol Cell. 2009 Aug;20(15):3451-8. d

Insert

stathmin (STMN1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pUNIV backbone

Plasmid

#24705

Depositor

Cynthia Czajkowski

Article

Venkatachalan et al Pflugers Arch. 2007 Apr . 454(1):155-63.

Type

Empty backbone

Use

Xenopus oocyte expression

Expression

Bacterial, Mammalian, and Y…

Available Since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

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NeonGreen-C1-ORP3

Plasmid

#220070

Purpose

overexpresses ORP3 with a neonGreen Fluorophore

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

OSBPL3 (OSBPL3 Human)

Expression

Mammalian

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-Hb9-RFP

Plasmid

#37081

Depositor

Fred Gage

Article

Marchetto et al Cell Stem Cell. 2008 Dec 4;3(6):649-57.

Insert

Hb9 RFP

Use

Lentiviral

Promoter

Hb9

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pFLAG-SURF4

Plasmid

#170680

Purpose

Expresses FLAG-tagged SURF4 in mammalian cells.

Depositor

Ke Xu

Article

Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

Insert

SURF4

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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