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pBbE0c-RFP

Plasmid

#35364

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 3, 2012

Availability

Academic Institutions and Nonprofits only

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pGC319

Plasmid

#19677

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R4-R3)

Use

Flp/frt, destination vector

Tags

4xNLS

Expression

Worm

Available Since

March 17, 2009

Availability

Academic Institutions and Nonprofits only

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pXDC61-LepA

Plasmid

#21843

Depositor

Howard Shuman

Article

Charpentier et al PLoS Pathog. 2009 Jul . 5(7):e1000501.

Insert

LepA (lepA L. pneumophila)

Tags

TEM

Expression

Bacterial

Available Since

Sept. 2, 2009

Availability

Academic Institutions and Nonprofits only

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pAB174

Plasmid

#101673

Purpose

Opto-T7RNAP*(179)

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(179)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAB144

Plasmid

#101665

Purpose

Opto-T7RNAP*(69)

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(69)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAB153

Plasmid

#101664

Purpose

Opto-T7RNAP*(600)

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(600)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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PAJ389

Plasmid

#104147

Purpose

dCas9 plasmid expressing 2A sRNA trigger

Depositor

Alfonso Jaramillo

Article

Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

pLtetO-1

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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PAJ390

Plasmid

#104148

Purpose

dCas9 plasmid expressing 3A sRNA trigger

Depositor

Alfonso Jaramillo

Article

Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

pLtetO-1

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pNOC-FLUC

Plasmid

#98140

Purpose

Gateway compatible construct for N' terminal fusion or promoter driven firefly luciferase reporter, hygromycin resistance cassette

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Tags

HA

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSB58 (Prp16)

Plasmid

#111282

Purpose

Prp16 in pSE358(Trp)

Depositor

Christine Guthrie

Article

Burgess et al Cell. 1993 Jul 2;73(7):1377-91.

Insert

PRP16

Expression

Yeast

Available Since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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pBsaG4SPdX

Plasmid

#108203

Purpose

G4S-PdX

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

Putidaredoxin

Expression

Bacterial

Mutation

changed Cysteine 73 to Serine and Cysteine 85 to …

Promoter

lac promoter

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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Ptrc::glgP_gap1

Plasmid

#101845

Purpose

Replaces native promoter of glgP_gap1 with Ptrc promoter

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

CmR casette followed by ptrc promoter

Use

Cloning vector

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCASCADE-fabI-gltA1-gltA2-udhA-gapAP1

Plasmid

#87154

Purpose

fabI-gltA1-gltA2-udhA-gapAP1 targeting guide RNA E. coli , Low Phosphate Induction

Depositor

Michael Lynch

Insert

fabI-gltA1-gltA2-udhA-GapAP1 guide array

Expression

Bacterial

Promoter

E . coli ugpB gene promoter, low phosphate induct…

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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pJPC12-ΔM13-mCherry-P/PM,5G6T-GFP

Plasmid

#80912

Purpose

Reporter plasmid (mCherry and GFP)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

mCherry
GFP

Expression

Bacterial

Promoter

P5G6T and PM,5G6T

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pNR163

Plasmid

#79544

Purpose

Output plasmid: contains 5-state register that regulates expression of GFP.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pWW2149

Plasmid

#80392

Purpose

Contains constitutive histidine kinase (pCon-Taz) with 4x SH3 scaffold domains.

Depositor

Richard Murray

Article

Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

Insert

Taz Histidine Kinase

Use

Synthetic Biology

Tags

Fused by GS linkers to 4 SH3 domains

Available Since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pJOG294

Plasmid

#80587

Purpose

dummy module (ccdB) for 2nd generation recipient assembly

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pNR165

Plasmid

#79646

Purpose

Output plasmid: contains 5-state register that regulates expression of GFP.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pNR187

Plasmid

#79553

Purpose

Output plasmid: contains 5-state register that regulates expression of GFP.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pNR164

Plasmid

#79545

Purpose

Output plasmid: contains 5-state register that regulates expression of GFP.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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