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pMGF157

Plasmid

#96972

Purpose

ish1-mCherry insertion cassette with Hygro resistance gene

Depositor

Adam Frost, Wesley Sundquist

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

ish1 (ish1 Fission Yeast)

Tags

GST and mCherry

Expression

Bacterial

Mutation

ish1 C-terminal domain fused with mCherry + hygro…

Promoter

N/A

Available Since

Aug. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pTH744-CEN-RLuc/slowmaxCFLuc

Plasmid

#38224

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

All codons of the original FLuc gene were exchang…

Promoter

ADH1 and TDH3 (=GPD)

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pET32b-MED19

Plasmid

#15438

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED19 (Med19 Mouse)

Tags

His and Trx

Expression

Bacterial

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pET41a-MED30

Plasmid

#15431

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED30 (Med30 Mouse)

Tags

GST and His

Expression

Bacterial

Mutation

N/A

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pTH742-CEN-RLuc/slowminCFLuc

Plasmid

#38222

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

All codons of the original FLuc gene were exchang…

Promoter

ADH1 and TDH3 (=GPD)

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pMGF170

Plasmid

#96997

Purpose

ish1-mCherry insertion cassette with NAT resistance gene

Depositor

Adam Frost, Wesley Sundquist

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

ish1 (ish1 Fission Yeast)

Tags

GST and mCherry

Expression

Bacterial

Mutation

ish1 C-terminal domain fused with mCherry + NAT r…

Promoter

N/A

Available Since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pET41a-MED31

Plasmid

#15437

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED31 (Med31 Mouse)

Tags

GST and His

Expression

Bacterial

Mutation

N/A

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
His-FLAG-Med18 pBacPAK8

Plasmid

#15371

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

Med18 (MED18 Human)

Use

Baculovirus construction

Tags

FLAG and His

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
His-FLAG-Med20 pBacPAK8

Plasmid

#15372

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

Med20 (Med20 Mouse)

Use

Baculovirus construction

Tags

FLAG and His

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
FLAG-Med11 pBacPAK8

Plasmid

#15373

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

Med11 (Med11 Mouse)

Use

Baculovirus construction

Tags

FLAG

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
myc-Med22 pBacPAK8

Plasmid

#15374

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

Med22 (MED22 Human)

Use

Baculovirus construction

Tags

myc

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
myc-Med31 pBacPAK8

Plasmid

#15375

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

Med31 (Med31 Mouse)

Use

Baculovirus construction

Tags

myc

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
myc-Med19 pBacPAK8

Plasmid

#15376

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

Med19 (Med19 Mouse)

Use

Baculovirus construction

Tags

myc

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pAvA139

Plasmid

#248145

Purpose

mutated LuxR transcriptional activator for expression in yeast: fused with Gal4 activation domain with 'gen1' mutations

Depositor

Emil Jensen

Article

van Aalst et al Commun Biol. 2025 Nov 27. doi: 10.1038/s

Insert

luxR (gen1 mutations)

Use

Integration vector

Tags

Gal4 activation domain and nuclear localization s…

Expression

Yeast

Mutation

Gal4_AD: N24K, P41 (CCA→CCG), N46D, T92S, V98 (GT…

Promoter

pPGK1

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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Aga2p-TEVcs-LacAnc100-myc_pCTCON2

Plasmid

#245298

Purpose

expresses LaccID on the yeast surface

Depositor

Alice Ting

Article

Lee et al Nat Chem Biol. 2025 Aug 1. doi: 10.1038/

Insert

Aga2p-LacAnc100

Expression

Yeast

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pJRoC30-LacAnc100

Plasmid

#234653

Purpose

Expresses LacAnc100 variant in yeast

Depositor

Miguel Alcalde Galeote, Alice Ting

Article

Lee et al Nat Chem Biol. 2025 Aug 1. doi: 10.1038/

Insert

LacAnc100

Tags

HRV 3C protease site-GSG linker-8xHis tag

Expression

Yeast

Promoter

Gal1p

Available Since

Aug. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-KAE1

Plasmid

#232891

Purpose

Plasmid expressing Cas9 and gRNA GATGACAACTGAATGCAGAG which targets the KAE1 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

KAE1 gRNA (KAE1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-SAM50

Plasmid

#232895

Purpose

Plasmid expressing Cas9 and gRNA AATAGTTTATGTAAGAACAG which targets the SAM50 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

SAM50 gRNA (SAM50 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3

Plasmid

#221112

Purpose

FAP tagged STE3 (N-terminally tagged, optimized for yeast expression) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

MFA-IgKappa-FAPoptim-STE3

Expression

Yeast

Promoter

STE3

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-ARB1

Plasmid

#232888

Purpose

Plasmid expressing Cas9 and gRNA CAAAAACTAACTGCTTACGG which targets the ARB1 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

ARB1 gRNA (ARB1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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