179,981 results
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Plasmid#136570PurposeLAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLAMP1-emiRFP670
TagsemiRFP670ExpressionMammalianPromoterCMVAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomato
Plasmid#113865PurposeTriple pathway reporter, 3P-Fluor; wnt-iRFP, hedgehog-mTurquoise2, notch-tdTomato; plus CMV-eGFP. Gateway expression vector for lentivirus generation.DepositorInsertsCMV-eGFP
TOP-iRFP
PTCH1-mTurquoise2
CBF-tdTomato
UseLentiviralExpressionMammalianAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lenti-Cas9-gRNA-TagBFP2
Plasmid#124774Purpose3rd generation lentiviral plasmid encoding Cas9, TagBFP2, and a gRNA backboneDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSH-EFIRES-P-AtAFB2-mCherry
Plasmid#129716PurposeAAVS1 safe harbor targeting vector expressing AtAFB2-mCherryDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAFB2 (AFB2 Mustard Weed)
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsmCherryExpressionMammalianMutationcodon optimizedPromoterEF1aAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-FAH-rtTA3G
Plasmid#120309PurposeAn AAV vector that expresses rtTA3G under the c-fos promoterDepositorInsertrtTA3G
UseAAVPromoterc-fosAvailable sinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Green Glifon50
Plasmid#126206Purposegreen fluorescent glucose sensor for mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGreen Glifon50
ExpressionMammalianAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-GRAB_NE1h
Plasmid#123309PurposeExpresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h in neuronsDepositorHas serviceAAV9 and AAV RetrogradeInsertGPCR activation based NE sensor GRAB_NE1h
UseAAVPromoterhSynAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSP2-96-merTetO-EFS-BLaR
Plasmid#118713PurposeContains an optimized 96-mer TetO repeat for imaging of desired lociDepositorInsertsBlasticidin resistance gene
EFS-NS promoter
ExpressionMammalianPromoterEFS-NSAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPUR-hU6-sgRNA-Sirius-8XMS2
Plasmid#121942PurposeCRISPR-Sirius plasmidDepositorInsertsgRNA-Sirius-8XMS2
UseCRISPR and LentiviralTagsnoExpressionMammalianPromoterhuman U6 promoterAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUAS-V5-TurboID-NES
Plasmid#116904PurposeExpresses TurboID under Gal4/UAS control. TurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTurboID
UseDrosophila expressionAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HA-14-3-3 zeta
Plasmid#116888PurposeExpresses HA-tagged human 14-3-3 zeta in mammalian cellsDepositorAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDB302
Plasmid#113673Purposeexpresses Cth SUMO protease in Escherichia coliDepositorInsertCth SUMO protease
Tags10-HisExpressionBacterialMutationdeleted amino acids 1-934PromoterT7Available sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-TIWB-myrmScarlet-I-P2A-post-eGRASP
Plasmid#111584PurposeAn AAV vector that expresses myristoylated mScarlet-I and post-eGRASP linked by self-cleaving P2A peptide under the tetO promoter.DepositorInsertmyrmScarlet-I-P2A-post-eGRASP
UseAAVPromotertetOAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))
Plasmid#105842PurposeDonor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichmentDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsUseCRISPR and TALENPromoterCAG, EF-1alpha, and TRE3GAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.CAG.LSL.EGFP
Plasmid#100047PurposeAAV mediated Cre-dependent expression of EGFP (Lox-Stop-Lox)DepositorInsertEGFP
UseAAV and Cre/LoxExpressionMammalianPromoterCAGAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC0050-CMV-dPspCas13b-longlinker-ADAR2DD(wt)
Plasmid#103866PurposedPspCas13b-ADAR2DD(wt) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsUseCRISPRTagsGSGGGGS and HIV NESExpressionMammalianMutationChanged histidne 133 to alanine and histidine 105…Available sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP SNAP23
Plasmid#101914PurposeExpresses SNAP23 in mammalian cellsDepositorAvailable sinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
htrpC6-YFP
Plasmid#21084DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTrpC6 (TRPC6 Human)
TagsYFPExpressionMammalianAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPapi
Plasmid#96921Purposeexpression of S aureus SaCas9 (SauCas9) and two pol III promoters for an Sa gRNA and an Sp gRNA. Intended to be used in SpCas9-expressing cell lines for dual Cas9 "Big Papi" screens. aka pXPR207.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
6His-MBP-TEV-huAsCpf1
Plasmid#90095PurposeBacterial expression plasmid for protein purificationDepositorInserthuAsCpf1
Tags3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…ExpressionBacterialAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by