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pFRTpsiCHECK_SEAP

Plasmid

#154461

Purpose

Expression of SEAP and firefly luciferase

Depositor

Markus Landthaler

Article

Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

Insert

SEAP

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pCWXPGR-pTF-Wnt3a

Plasmid

#114287

Purpose

TET-inducible expression of Wnt3a

Depositor

Patrick Salmon

Article

Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

Insert

Wnt3a (Wnt3a Mouse)

Use

Lentiviral

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 KBTBD4 R313PRR

Plasmid

#184629

Purpose

Mammalian expression vector with N-terminal Flag for KBTBD4 R313PRR expression in mammalian cells

Depositor

Vincenzo D'Angiolella

Article

Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

Insert

N-terminal 3xFlag tagged KBTBD4 R313PRR (KBTBD4 Human)

Expression

Mammalian

Mutation

R313PRR

Promoter

CMV

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAAVLINKv1-pCALM1-Cre-3'-SYNGAP1-FLAG

Plasmid

#244265

Purpose

3' AAVLINK plasmid for SYNGAP1 expression

Depositor

Zhonghua Lu

Article

Lin et al Cell. 2026 Feb 5;189(3):969-986.e17. doi

Insert

SYNGAP1 (SYNGAP1 Human)

Use

AAV

Tags

FLAG

Promoter

pCALM1

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pHL-sec

Plasmid

#99845

Purpose

Mammalian expression with secretion signal sequence

Depositor

Edith Yvonne Jones

Article

Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

Insert

ephrin b2 (EFNB2 Human)

Tags

His

Expression

Mammalian

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pFV2-HA

Plasmid

#187082

Purpose

Expression of ATP synthase, with all Cys changed to Ala, and subunit a-HA tagged, subunit beta-His tagged)

Depositor

Steven Vik

Article

Bae et al Biochim Biophys Acta. 2009 Sep;1787(9):1

Insert

atpBEFHAGDC

Tags

6-His beta subunit (atpD) and HA tag subunit a (a…

Expression

Bacterial

Mutation

All Cys codons changed to Ala.

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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gRNA_Cloning Vector

Plasmid

#41824

Purpose

An empty gRNA expression vector, used to create a gRNA to a specific sequence.

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Tags

AflII site for Gibson Assembly

Expression

Mammalian

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-EGFP (AAV Retrograde)

Viral Prep

#50469-AAVrg

Purpose

Ready-to-use AAV Retrograde particles produced from pAAV-CaMKIIa-EGFP (#50469). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-EGFP plasmid DNA. CamKIIa-driven EGFP expression control. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

Depositor

Bryan Roth

Promoter

CaMKII

Tags

EGFP

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only
pYJ21-lenti-EF1a-ASAP2f-puro

Plasmid

#131065

Purpose

Forced expression of ASAP2f

Depositor

Xiaojun Lian

Article

Jiang et al Stem Cell Res Ther. 2021 May 29;12(1):30

Insert

ASAP2f

Use

Lentiviral

Expression

Mammalian

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti_MCP-p300_Hygro

Plasmid

#138459

Purpose

lenti vector encoding the MS2-p300 with a 2A Hygro resistance marker

Depositor

Jian Xu

Article

Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

Insert

p300 core

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1A

Available Since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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mCh-KIF5A*-strep

Plasmid

#120163

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF5A-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 5A (Kif5a Mouse)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF5A: 1-559 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag PLAU

Plasmid

#20588

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PLAU (PLAU Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pLentiCRISPRv2-Stuffer-HepmCherry

Plasmid

#192825

Purpose

Contains stuffer sequence into which sgRNAs can be cloned and expresses mCherry from hepatocyte-specific promoter

Depositor

Kristin Knouse

Article

Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

Insert

mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Hepatocyte-specific promoter (HS-CRM8-TTRmin modu…

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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BS-Ura3-GFP-Atg8

Plasmid

#69194

Purpose

Single copy integration of GFP-Atg8, use auxotrophic marker URA3(Kluyveromyces lactis)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

GFP-Atg8

Tags

EGFP

Expression

Yeast

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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A*11:01:01

Plasmid

#203281

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*11:01:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pME-ERT2-Cre-ERT2

Plasmid

#82587

Purpose

Middle entry vector containing tamoxifen-inducible estrogen receptor (ERT2) fused to both ends of CRE recombinase for tamoxifen-inducible Cre recombination

Depositor

Kryn Stankunas

Article

Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

Insert

ERT2-CRE-ERT2

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Puro DEST PKAKTRClover

Plasmid

#59153

Purpose

Lentiviral vector to express PKA KTR mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

PKA (PRKACA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

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DT480

Plasmid

#80413

Purpose

Expresses 480 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

Depositor

Thomas Cooper

Article

Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

Insert

human DMPK exons 11-15 with 480 interrupted CTG repeats (DMPK Human)

Expression

Mammalian

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-jGCaMP8m

Plasmid

#213010

Purpose

To study calcium signaling in Astrocytes

Depositor

Baljit Khakh

Article

Ollivier et al Nature. 2024 Mar;627(8003):358-366. doi:

Insert

GfaABC1D-jGCaMP8m

Use

AAV

Promoter

GfaABC1D

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pILGFP3AG4

Plasmid

#185874

Purpose

A plasmid for in vivo gene amplification (HapAmp) at RPL25 locus (~12 copy)

Depositor

Claudia Vickers

Article

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Type

Empty backbone

Expression

Yeast

Mutation

None

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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