We narrowed to 11,392 results for: ARIA

1. AT-U1

   Plasmid

   #112059

   Purpose

   U1 snRNA tagged with 1 copy of Riboglow (truncated variant A)

   Depositor

   Amy Palmer

   Article

   Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

   Insert

   U1 (RNU1-1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   1 copy of Riboglow tag (truncated variant A) near…

   Promoter

   Available since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDNR-SpCas9-Gem

   Plasmid

   #80424

   Purpose

   Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining

   Depositor

   Sara Howden, Melissa Little

   Article

   Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

   Insert

   SpCas9-Gem
   

   Use

   CRISPR

   Tags

   Geminin

   Expression

   Mutation

   codon optimized for human

   Promoter

   T7

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-GFP-EphrinB3-Flag

   Plasmid

   #155012

   Purpose

   Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 from pcDNA3.1

   Depositor

   Sinisa Urban

   Article

   Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

   Insert

   EphrinB3 (EPHB3 Human)
   

   Use

   Tags

   Flag, GFP, and signal peptide (residues 1 to 32) …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 SARS-CoV-2 S D614

   Plasmid

   #158074

   Purpose

   Expresses SARS-CoV-2 spike protein

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   SARS-CoV-2 spike (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPGK-T7/2-CD44v6 (fl)

   Plasmid

   #137816

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44v6 (fl) (CD44 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   R296K in the V6 region [Please see depositor comm…

   Promoter

   PGK

   Available since

   June 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPGK-T7/2-CD44v6-10 (fl)

   Plasmid

   #137820

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44v6-10 (fl) (CD44 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   full length

   Promoter

   PGK

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.hSynapsin.SF-iGluSnFR.A184V

   Plasmid

   #106175

   Purpose

   Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-iGluSnFR.A184V
   

   Use

   AAV

   Tags

   Expression

   Mutation

   GltI: A184V

   Promoter

   hSynapsin

   Available since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXs-hM₃O-IP

   Plasmid

   #46645

   Depositor

   Nobuaki Kikyo

   Article

   Hirai et al Stem Cells. 2011 Sep;29(9):1349-61. doi:

   Insert

   hM₃O (POU5F1 Human)
   

   Use

   Retroviral

   Tags

   IRES Puro and human MyoD (aa 1-62)

   Expression

   Mammalian

   Mutation

   contains a fragment of human MyoD (aa1-62) fused …

   Promoter

   Available since

   July 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB-cT3G-cERP2-PPARG

   Plasmid

   #192912

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of PPARG

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   PPARG (PPARG Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Luciferase

    Plasmid

    #213979

    Purpose

    Expresses Doxy Inducible Luciferase

    Depositor

    Coleman Lindsley

    Article

    Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

    Insert

    Luciferase
    

    Use

    Lentiviral

    Tags

    6xHis affinity tag

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Tet-responsive promoter PTight, consisting of sev…

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDF-Az

    Plasmid

    #197101

    Purpose

    To express the azidophenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into azidophenylalanine

    Depositor

    Kensaku Sakamoto

    Article

    Yamaguchi et al Bioconjug Chem. 2016 Jan 20;27(1):198-20

    Insert

    TyrRS variant, amber suppressor tRNA, kanamycin resistance gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Thr32, Asn107, Pro158, Leu159, Gln162, Arg286 (Ty…

    Promoter

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pαH-S-GSAS

    Plasmid

    #164565

    Purpose

    SARS-CoV-2 Spike protein (S-GSAS variant)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

    Insert

    Spike (S-GSAS variant)
    

    Use

    Tags

    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. evoFERNY-Cas9NG

    Plasmid

    #203329

    Purpose

    Plasmid for bacterial purification of codon optimized evoFERNY-Cas9NG

    Depositor

    Vijay Sankaran

    Article

    Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

    Insert

    evoFERNY-Cas9NG
    

    Use

    CRISPR

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-RR-ABE8e-V106W

    Plasmid

    #193656

    Purpose

    Expression of base editor RR-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-TadA*8e-V106W, EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dLbCas12a-G532R/K595R, TadA*8e-V106W

    Promoter

    CMV

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-T7-SpCas9-HF1-P2A-EGFP (RTW3505)

    Plasmid

    #139995

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-HF1(N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9-HF1 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    HF1=N497A/R661A/Q695A/Q926A

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTwist-SARS-CoV-2 Δ18 B.1.351v2

    Plasmid

    #169463

    Purpose

    Encodes SARS-CoV-2 B.1.351v2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 B.1.351v2 Spike truncated to remove 18 amino acids (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. HDM_Spikedelta21_sinobiological_B.1.617.2

    Plasmid

    #204149

    Purpose

    Expression plasmid for SARS-CoV-2 Delta variant spike

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Insert

    SARS-CoV-2 Delta variant spike (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    21 amino acid cytoplasmic tail deletion

    Promoter

    CMV

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTwist-SARS-CoV-2 Δ18 B.1.1.7

    Plasmid

    #169459

    Purpose

    Encodes SARS-CoV-2 B.1.1.7 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 B.1.1.7 Spike truncated to remove 18 amino acids (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-dCas12a-A3A-N57G

    Plasmid

    #193639

    Purpose

    Expression of base editor dCas12a-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-hA3A-N57G, EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hAPOBEC3A-N57G

    Promoter

    CMV

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-dCas12a-A3A-Y130F

    Plasmid

    #193640

    Purpose

    Expression of base editor dCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-hA3A-Y130F, EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hAPOBEC3A-Y130F

    Promoter

    CMV

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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