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We narrowed to 83,772 results for: Mycs

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Showing: 6841 - 6860 of 83772 results

  1. pCfB8709

    Plasmid
    #175224
    Purpose
    Xenopus oocyte expression vector containing YOL092W. Contains T7 RNA polymerase binding site, and adds polyA tail to YOL092W. Used for generation of mRNA, for injection into xenopus laevis oocytes.
    Depositor
    Irina Borodina
    Article
    Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.
    Insert
    YOL092W (CEN.PK strain)
    Use
    Mrna expression, injection into xenopus oocytes
    Tags
    PolyA
    Promoter
    T7
    Available Since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    175224-plasmid-map-sequence-id-347995

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  3. TFORF3447

    Plasmid
    #144923
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MYCBP (MYCBP Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144923-plasmid-map-sequence-id-404493

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  5. pDIV495

    Plasmid
    #177702
    Purpose
    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii
    Depositor
    Uffe Mortensen
    Article
    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0
    Inserts
    Cas9
    Nat
    sgRNA Targeting ADE2 locus in D.hansenii: AGCTAaGCAGATTAAtGCAT
    Use
    CRISPR
    Tags
    SV40
    Expression
    Bacterial and Yeast
    Promoter
    RNR2p (Debaryomyces hansenii ), SRC1p (Debaryomyc…
    Available Since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    177702-plasmid-map-sequence-id-353319

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  6. pPodA11

    Plasmid
    #162843
    Purpose
    Expression protein designed PodA10 from a TEV cleavable IPTG inducible plasmid with ampicillin resistance
    Depositor
    Dianne Newman
    Article
    VanDrisse et al Proc Natl Acad Sci U S A. 2021 Mar 23;11
    Insert
    Protein Designed Pyocyanin demethylase
    Tags
    TEV-cleavable 6x-His tag
    Expression
    Bacterial
    Mutation
    A53N, I73T, A87V, M99V, A129T from WT PodA
    Promoter
    T7
    Available Since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    162843-plasmid-map-sequence-id-343491

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  11. pML107

    Plasmid
    #67639
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation
    Depositor
    John Wyrick
    Article
    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1
    Inserts
    Cas9
    single guide RNA expression cassette
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    pSNR52 and pTDH3 (aka GAP promoter)
    Available Since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67639-plasmid-map-sequence-id-220324

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  13. pLV-dCas9-KRAB-PGK-HygR

    Plasmid
    #83890
    Purpose
    Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.
    Depositor
    Charles Gersbach
    Article
    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038
    Inserts
    humanized dead Cas9 KRAB
    aminoglycoside phosphotransferase from E. coli
    Use
    CRISPR and Lentiviral
    Tags
    Flag
    Mutation
    D10A and H840A
    Promoter
    Human Ubiquitin C Promoter and mouse phosphoglyce…
    Available Since
    April 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    83890-plasmid-map-sequence-id-240902

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  14. hZMPSTE24-3FLAG_pQCXIP

    Plasmid
    #89759
    Purpose
    Human ZMPSTE24 mammalian expression vector. Retroviral bicistronic puromycin vector.
    Depositor
    Martin Dorf
    Article
    Fu et al J Exp Med. 2017 Feb 28. pii: jem.2016127
    Insert
    Homo sapiens zinc metallopeptidase STE24 (ZMPSTE24) (ZMPSTE24 Human)
    Use
    Retroviral; Bicistronic expression: puromycin re…
    Tags
    9bp spacer (NotI site) - 3xFLAG epitopes - Stop c…
    Expression
    Mammalian
    Mutation
    Y253H in ZMPSTE24 (see depositor comments below)
    Promoter
    CMV
    Available Since
    June 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    89759-plasmid-map-sequence-id-180605

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  15. pHSN6I01

    Plasmid
    #50587
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    dCas9-KRAB
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Mutation
    dCas9 that is defective in DNA cleavage; the maiz…
    Promoter
    2×35Sp and AtU6-26p
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50587-plasmid-map-sequence-id-75659

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  16. UbC-blasticidine-P2A-TETon-3G transactivator

    Plasmid
    #200191
    Purpose
    A TETon activator, which combined with the DiLiCre 2.0 plasmid, drives the expression of DiLiCre 2.0 upon doxycycline. DiLiCre 2.0 is a cre recombinase that is activated by violet light.
    Depositor
    Jacco van Rheenen
    Article
    Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:
    Insert
    blasticidine - P2A - TETon-3G transactivator
    Use
    Lentiviral
    Expression
    Bacterial and Mammalian
    Promoter
    UbC
    Available Since
    Aug. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    200191-plasmid-map-sequence-id-405986

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  17. p426_Cas9_gRNA-HIS3b

    Plasmid
    #87402
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting HIS3b sequence AATATAGAGTGTACTAGAGG in yeast chromosome 15.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting HIS3b
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available Since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87402-plasmid-map-sequence-id-508301

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  18. p426_Cas9_gRNA-ARS1309a

    Plasmid
    #87399
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1309a sequence CCTGTGGTGACTACGTATCC in yeast chromosome 13.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1309a
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available Since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87399-plasmid-map-sequence-id-508300

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  19. p426_Cas9_gRNA-YOLCd1b

    Plasmid
    #87401
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting YOLCd1b sequence GACTAGTTAAGCGAGCATGT in yeast chromosome 15.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting YOLCd1b
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available Since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87401-plasmid-map-sequence-id-508308

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  20. p426_Cas9_gRNA-ARS1414a

    Plasmid
    #87400
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1414a sequence GCGCCACAGTTTCAAGGGTC in yeast chromosome 14.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1414a
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available Since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87400-plasmid-map-sequence-id-508307

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Showing: 6841 - 6860 of 83772 results