We narrowed to 11,947 results for: emb
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Plasmid#193369PurposeExpression of C9ORF78 F8A variant in E.coli with N-terminal His6-GST-tag which is cleavable with TEV proteaseDepositorInsertC9ORF78_F8A (C9orf78 Human)
TagsHis6-GST-TEVExpressionBacterialMutationF8A, the mutation is located in the binding inter…PromoterT7Available sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT9_i1-14-GFP10
Plasmid#180343Purposemammalian expression of human SEPT9_i1 fused to GFP10DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSEPTIN9_v1 (SEPTIN9 Human)
TagsGFP10ExpressionMammalianPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-Ensconsin-3XGFP-stop-L2
Plasmid#186361PurposeEntry clone with ORF encoding the MT-binding domain of Ensconsin fused to 3 copies of GFP in C-terminal, flanked by Gateway recombination sequences.DepositorPublicationInsertHuman Ensconsin (MAP7 Synthetic, Human)
UseExpression of a fluorescent microtubule markerTags3xEGFPAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTub-alpha1-iLifeact-NeonGreen
Plasmid#175258PurposeF-actin structures labellingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertiLifeact
TagsmNeonGreenExpressionMammalianMutationNone (wt)PromoterpTub-alpha1Available sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Ki-67(RASA)-mCherry_IRESpuro2
Plasmid#182921PurposeExpresses EGFP-Ki-67(RASA)-mCherry. Ki67 contains RASA mutation.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMKI67 (MKI67 Human)
TagsEGFP and mCherryExpressionBacterial and MammalianMutationRASA mutationPromoterCMVAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPyCAG-NE-EGFP-IRES-Pac
Plasmid#183617PurposeMammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered EGFP (NLS-EGFP-EmerinTMD). Confers puromycin resistance.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP
TagsHuman EMD transmembrane domain and NLSExpressionMammalianPromoterCAGAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cry2PHR - IBAR
Plasmid#176602PurposeCry2PHR with C-terminal IBAR domainDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCry2PHR-mCherry-IBAR
ExpressionMammalianAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Halo-WIPI2B L69E
Plasmid#175026PurposeExpresses WIPI2B (L69E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertWIPI2B (L69E) (WIPI2 Human)
TagsHaloTagExpressionMammalianMutationLeucine 69 to Glutamate (CTA to GAA)PromoterCMVAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Halo-WIPI2B H85E
Plasmid#175027PurposeExpresses WIPI2B (H85E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertWIPI2B (H85E) (WIPI2 Human)
TagsHaloTagExpressionMammalianMutationHistadine 85 to Glutamate (CAC to GAG)PromoterCMVAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T Doc2beta C2AB(125) G361C
Plasmid#134694PurposeEncodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labelingDepositorInsertDouble C2 protein beta (Doc2b Rat)
TagsGSTExpressionBacterialMutationG361C, C145A, C217A, C249A, C290A, C338A, C387AAvailable sinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-WIPI2d10-364delta263-295-TEV-STREP
Plasmid#171830PurposeMammalian expression of human WIPI2d 10-364 delta263-295 with C-terminal StrepDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertWIPI2d (WIPI2 Human)
TagsStrepExpressionMammalianMutation10-364delta263-295Available sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH_CMV_dimer_mKate2
Plasmid#167333PurposeExpresses mKate2 fused to TFAM no-dimerization mutant in mammalian cellsDepositorInsertTFAM (TFAM Human)
UseLentiviralTagsmKate2ExpressionMammalianMutationChanged K95A, Y99F, E106A, E112A and R116APromoterCMVAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH_CMV_HMGA_mKate2
Plasmid#167334PurposeExpresses mKate2 fused to TFAM HMGA mutant in mammalian cellsDepositorInsertTFAM (TFAM Human)
UseLentiviralTagsmKate2ExpressionMammalianMutationTruncation mutant containing MTS (1-42) and HMGA …PromoterCMVAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 AtU6-26:LbCas12a_DR (GB1443)
Plasmid#160575PurposeLbCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAtU6-26:LbCas12a_DR
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removedPromoterU6-26Available sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWPXL-p150-MIRΔ
Plasmid#160995PurposeExpress mouse p150 protein with MIR deletion mutationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCHAF1A (Chaf1a Mouse)
TagsGFPExpressionMammalianMutationMIR (HP1-interacting domain) deletionAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgDnmt1_1
Plasmid#138347PurposeExpress guide RNA 1 for Dnmt1DepositorInsertmouse Dnmt1 sgRNA (Dnmt1 Synthetic)
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgDnmt1_2
Plasmid#138348PurposeExpress guide RNA 2 for mouse Dnmt1DepositorInsertmouse Dnmt1 sgRNA (Dnmt1 Synthetic)
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFLinkC-AI(GPG)-FKBP12(P9)-FRB(GPG)-TVMV
Plasmid#137118PurposeProtease Switch Clone ID: 2-B3DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAI(GPG)-FKBP12(P9)-FRB(GPG)-TVMV
UseSynthetic BiologyAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-cGASΔN C396/7A-HA
Plasmid#130918PurposeExpresses human cGASΔN (aa160-522)-HA with C396/397A point mutation; Puromycin selection markerDepositorInsertcGASΔN C396/7A
TagsHAExpressionMammalianMutationmutated residues 396 and 397 to alaninesPromoterCMVAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZIP-hCMV-ZsGreen-P2A-Puro-NT4
Plasmid#123328PurposeLentiviral vector for expression of non-targeting control shRNA #4 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insertDepositorInsertnon-targeting control shRNA embedded in miR30 backbone
UseLentiviralExpressionMammalianPromoterCMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only